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Adding nutrients to the biocontrol strain JK-SH007 promotes biofilm formation and improves resistance to stress
Burkholderia pyrrocinia JK-SH007 is an important biocontrol strain for the prevention and treatment of poplar canker disease. Its powerful biocontrol function is inseparable from its successful colonization of poplar trees. Bacterial biofilms can ensure the long-term colonization of a host. To explo...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013030/ https://www.ncbi.nlm.nih.gov/pubmed/32048076 http://dx.doi.org/10.1186/s13568-019-0929-8 |
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author | Fu, Huanhuan Chen, Feifei Liu, Wanhui Kong, Weiliang Wang, Chaoen Fang, Xueqi Ye, Jianren |
author_facet | Fu, Huanhuan Chen, Feifei Liu, Wanhui Kong, Weiliang Wang, Chaoen Fang, Xueqi Ye, Jianren |
author_sort | Fu, Huanhuan |
collection | PubMed |
description | Burkholderia pyrrocinia JK-SH007 is an important biocontrol strain for the prevention and treatment of poplar canker disease. Its powerful biocontrol function is inseparable from its successful colonization of poplar trees. Bacterial biofilms can ensure the long-term colonization of a host. To explore the mechanism of action of biofilms in the biocontrol process, we manipulated various exogenous factors to explore the morphology of the JK-SH007 biofilm in vitro. The addition of glycerol and MgSO(4) to TSB medium stimulated biofilm production, increased the resistance of JK-SH007 to disease, enhanced the survival of JK-SH007 in nutrient-poor environments and maintained the antagonistic ability of JK-SH007 against the poplar canker pathogen. Therefore, we constructed and optimized a biofilm-forming system to produce a large number of stable JK-SH007 biofilms. The optimized system showed that the optimal incubation time for JK-SH007 biofilm formation was 14 h, the optimal temperature of the static culture was 25 °C, and the optimal pH was 5. The optimal medium for biofilm formation was TSB medium, 1% glycerol and 50 mM MgSO(4). RT-qPCR experiments showed that an increase in the expression of the suhB gene promoted JK-SH007 biofilm formation, while an increase in the expression level of the ropN gene inhibited JK-SH007 biofilm formation. The possible mechanism by which JK-SH007 was inhibited by biofilm formation under natural culture was revealed. These results indicate the importance of adding nutrients to JK-SH007 biocides produced on a commercial scale. This is the first report of JK-SH007 producing a long-lasting biofilm that guarantees antagonism. |
format | Online Article Text |
id | pubmed-7013030 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-70130302020-02-25 Adding nutrients to the biocontrol strain JK-SH007 promotes biofilm formation and improves resistance to stress Fu, Huanhuan Chen, Feifei Liu, Wanhui Kong, Weiliang Wang, Chaoen Fang, Xueqi Ye, Jianren AMB Express Original Article Burkholderia pyrrocinia JK-SH007 is an important biocontrol strain for the prevention and treatment of poplar canker disease. Its powerful biocontrol function is inseparable from its successful colonization of poplar trees. Bacterial biofilms can ensure the long-term colonization of a host. To explore the mechanism of action of biofilms in the biocontrol process, we manipulated various exogenous factors to explore the morphology of the JK-SH007 biofilm in vitro. The addition of glycerol and MgSO(4) to TSB medium stimulated biofilm production, increased the resistance of JK-SH007 to disease, enhanced the survival of JK-SH007 in nutrient-poor environments and maintained the antagonistic ability of JK-SH007 against the poplar canker pathogen. Therefore, we constructed and optimized a biofilm-forming system to produce a large number of stable JK-SH007 biofilms. The optimized system showed that the optimal incubation time for JK-SH007 biofilm formation was 14 h, the optimal temperature of the static culture was 25 °C, and the optimal pH was 5. The optimal medium for biofilm formation was TSB medium, 1% glycerol and 50 mM MgSO(4). RT-qPCR experiments showed that an increase in the expression of the suhB gene promoted JK-SH007 biofilm formation, while an increase in the expression level of the ropN gene inhibited JK-SH007 biofilm formation. The possible mechanism by which JK-SH007 was inhibited by biofilm formation under natural culture was revealed. These results indicate the importance of adding nutrients to JK-SH007 biocides produced on a commercial scale. This is the first report of JK-SH007 producing a long-lasting biofilm that guarantees antagonism. Springer Berlin Heidelberg 2020-02-11 /pmc/articles/PMC7013030/ /pubmed/32048076 http://dx.doi.org/10.1186/s13568-019-0929-8 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Fu, Huanhuan Chen, Feifei Liu, Wanhui Kong, Weiliang Wang, Chaoen Fang, Xueqi Ye, Jianren Adding nutrients to the biocontrol strain JK-SH007 promotes biofilm formation and improves resistance to stress |
title | Adding nutrients to the biocontrol strain JK-SH007 promotes biofilm formation and improves resistance to stress |
title_full | Adding nutrients to the biocontrol strain JK-SH007 promotes biofilm formation and improves resistance to stress |
title_fullStr | Adding nutrients to the biocontrol strain JK-SH007 promotes biofilm formation and improves resistance to stress |
title_full_unstemmed | Adding nutrients to the biocontrol strain JK-SH007 promotes biofilm formation and improves resistance to stress |
title_short | Adding nutrients to the biocontrol strain JK-SH007 promotes biofilm formation and improves resistance to stress |
title_sort | adding nutrients to the biocontrol strain jk-sh007 promotes biofilm formation and improves resistance to stress |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013030/ https://www.ncbi.nlm.nih.gov/pubmed/32048076 http://dx.doi.org/10.1186/s13568-019-0929-8 |
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