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Dataset for sequencing and de novo assembly of the European endangered white-clawed crayfish (Austropotamobius pallipes) abdominal muscle transcriptome
The white-clawed crayfish (Austropotamobius pallipes) is an endangered species in Europe with limited genomic information. Despite its conservation status there is no transcriptomic data available for A. pallipes in public databases. The data here represents the first transcriptome profile of the wh...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013156/ https://www.ncbi.nlm.nih.gov/pubmed/32071963 http://dx.doi.org/10.1016/j.dib.2020.105166 |
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author | Grandjean, Frederic Gan, Han Ming Moumen, Bouziane Giraud, Isabelle Hatira, Skander Cordaux, Richard Austin, Christopher M. |
author_facet | Grandjean, Frederic Gan, Han Ming Moumen, Bouziane Giraud, Isabelle Hatira, Skander Cordaux, Richard Austin, Christopher M. |
author_sort | Grandjean, Frederic |
collection | PubMed |
description | The white-clawed crayfish (Austropotamobius pallipes) is an endangered species in Europe with limited genomic information. Despite its conservation status there is no transcriptomic data available for A. pallipes in public databases. The data here represents the first transcriptome profile of the white-clawed crayfish generated using Illumina stranded RNA sequencing. Pair-end reads were assembled de novo with three separate transcriptome assemblers (Trinity, RNABloom, and RNASpades) followed by transcript assembly reduction and gene reconstruction using the EvidentialGene pipeline. The transcriptome was functionally annotated using InterProScan and genes coding for carbohydrate-active enzymes were identified through the dbCAN2 server. Raw fastq reads and the final version of the transcriptome assembly have been deposited in the NCBI-SRA (SRR10549898) and NCBI-TSA (GICG01) databases. |
format | Online Article Text |
id | pubmed-7013156 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-70131562020-02-18 Dataset for sequencing and de novo assembly of the European endangered white-clawed crayfish (Austropotamobius pallipes) abdominal muscle transcriptome Grandjean, Frederic Gan, Han Ming Moumen, Bouziane Giraud, Isabelle Hatira, Skander Cordaux, Richard Austin, Christopher M. Data Brief Genetics, Genomics and Molecular Biology The white-clawed crayfish (Austropotamobius pallipes) is an endangered species in Europe with limited genomic information. Despite its conservation status there is no transcriptomic data available for A. pallipes in public databases. The data here represents the first transcriptome profile of the white-clawed crayfish generated using Illumina stranded RNA sequencing. Pair-end reads were assembled de novo with three separate transcriptome assemblers (Trinity, RNABloom, and RNASpades) followed by transcript assembly reduction and gene reconstruction using the EvidentialGene pipeline. The transcriptome was functionally annotated using InterProScan and genes coding for carbohydrate-active enzymes were identified through the dbCAN2 server. Raw fastq reads and the final version of the transcriptome assembly have been deposited in the NCBI-SRA (SRR10549898) and NCBI-TSA (GICG01) databases. Elsevier 2020-01-27 /pmc/articles/PMC7013156/ /pubmed/32071963 http://dx.doi.org/10.1016/j.dib.2020.105166 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Genetics, Genomics and Molecular Biology Grandjean, Frederic Gan, Han Ming Moumen, Bouziane Giraud, Isabelle Hatira, Skander Cordaux, Richard Austin, Christopher M. Dataset for sequencing and de novo assembly of the European endangered white-clawed crayfish (Austropotamobius pallipes) abdominal muscle transcriptome |
title | Dataset for sequencing and de novo assembly of the European endangered white-clawed crayfish (Austropotamobius pallipes) abdominal muscle transcriptome |
title_full | Dataset for sequencing and de novo assembly of the European endangered white-clawed crayfish (Austropotamobius pallipes) abdominal muscle transcriptome |
title_fullStr | Dataset for sequencing and de novo assembly of the European endangered white-clawed crayfish (Austropotamobius pallipes) abdominal muscle transcriptome |
title_full_unstemmed | Dataset for sequencing and de novo assembly of the European endangered white-clawed crayfish (Austropotamobius pallipes) abdominal muscle transcriptome |
title_short | Dataset for sequencing and de novo assembly of the European endangered white-clawed crayfish (Austropotamobius pallipes) abdominal muscle transcriptome |
title_sort | dataset for sequencing and de novo assembly of the european endangered white-clawed crayfish (austropotamobius pallipes) abdominal muscle transcriptome |
topic | Genetics, Genomics and Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013156/ https://www.ncbi.nlm.nih.gov/pubmed/32071963 http://dx.doi.org/10.1016/j.dib.2020.105166 |
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