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Mechanical Model for Catch-Bond-Mediated Cell Adhesion in Shear Flow

Catch bond, whose lifetime increases with applied tensile force, can often mediate rolling adhesion of cells in a hydrodynamic environment. However, the mechanical mechanism governing the kinetics of rolling adhesion of cells through catch-bond under shear flow is not yet clear. In this study, a mec...

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Detalles Bibliográficos
Autores principales: Li, Long, Kang, Wei, Wang, Jizeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013535/
https://www.ncbi.nlm.nih.gov/pubmed/31963253
http://dx.doi.org/10.3390/ijms21020584
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author Li, Long
Kang, Wei
Wang, Jizeng
author_facet Li, Long
Kang, Wei
Wang, Jizeng
author_sort Li, Long
collection PubMed
description Catch bond, whose lifetime increases with applied tensile force, can often mediate rolling adhesion of cells in a hydrodynamic environment. However, the mechanical mechanism governing the kinetics of rolling adhesion of cells through catch-bond under shear flow is not yet clear. In this study, a mechanical model is proposed for catch-bond-mediated cell adhesion in shear flow. The stochastic reaction of bond formation and dissociation is described as a Markovian process, whereas the dynamic motion of cells follows classical analytical mechanics. The steady state of cells significantly depends on the shear rate of flow. The upper and lower critical shear rates required for cell detachment and attachment are extracted, respectively. When the shear rate increases from the lower threshold to the upper threshold, cell rolling became slower and more regular, implying the flow-enhanced adhesion phenomenon. Our results suggest that this flow-enhanced stability of rolling adhesion is attributed to the competition between stochastic reactions of bonds and dynamics of cell rolling, instead of force lengthening the lifetime of catch bonds, thereby challenging the current view in understanding the mechanism behind this flow-enhanced adhesion phenomenon. Moreover, the loading history of flow defining bistability of cell adhesion in shear flow is predicted. These theoretical predictions are verified by Monte Carlo simulations and are related to the experimental observations reported in literature.
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spelling pubmed-70135352020-03-09 Mechanical Model for Catch-Bond-Mediated Cell Adhesion in Shear Flow Li, Long Kang, Wei Wang, Jizeng Int J Mol Sci Article Catch bond, whose lifetime increases with applied tensile force, can often mediate rolling adhesion of cells in a hydrodynamic environment. However, the mechanical mechanism governing the kinetics of rolling adhesion of cells through catch-bond under shear flow is not yet clear. In this study, a mechanical model is proposed for catch-bond-mediated cell adhesion in shear flow. The stochastic reaction of bond formation and dissociation is described as a Markovian process, whereas the dynamic motion of cells follows classical analytical mechanics. The steady state of cells significantly depends on the shear rate of flow. The upper and lower critical shear rates required for cell detachment and attachment are extracted, respectively. When the shear rate increases from the lower threshold to the upper threshold, cell rolling became slower and more regular, implying the flow-enhanced adhesion phenomenon. Our results suggest that this flow-enhanced stability of rolling adhesion is attributed to the competition between stochastic reactions of bonds and dynamics of cell rolling, instead of force lengthening the lifetime of catch bonds, thereby challenging the current view in understanding the mechanism behind this flow-enhanced adhesion phenomenon. Moreover, the loading history of flow defining bistability of cell adhesion in shear flow is predicted. These theoretical predictions are verified by Monte Carlo simulations and are related to the experimental observations reported in literature. MDPI 2020-01-16 /pmc/articles/PMC7013535/ /pubmed/31963253 http://dx.doi.org/10.3390/ijms21020584 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Long
Kang, Wei
Wang, Jizeng
Mechanical Model for Catch-Bond-Mediated Cell Adhesion in Shear Flow
title Mechanical Model for Catch-Bond-Mediated Cell Adhesion in Shear Flow
title_full Mechanical Model for Catch-Bond-Mediated Cell Adhesion in Shear Flow
title_fullStr Mechanical Model for Catch-Bond-Mediated Cell Adhesion in Shear Flow
title_full_unstemmed Mechanical Model for Catch-Bond-Mediated Cell Adhesion in Shear Flow
title_short Mechanical Model for Catch-Bond-Mediated Cell Adhesion in Shear Flow
title_sort mechanical model for catch-bond-mediated cell adhesion in shear flow
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013535/
https://www.ncbi.nlm.nih.gov/pubmed/31963253
http://dx.doi.org/10.3390/ijms21020584
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