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Effect of the Application of a Dehydrothermal Treatment on the Structure and the Mechanical Properties of Collagen Film

Dehydrothermal (DHT) treatment was used to improve the properties of collagen casings because of its non-cytotoxicity. Understanding the effects of DHT treatment on the structure and mechanical properties of collagen films is beneficial to developing satisfying collagen casings. Herein, DHT treatmen...

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Autores principales: Chen, Xuefei, Zhou, Lingling, Xu, Huaizhong, Yamamoto, Masaki, Shinoda, Masaya, Kishimoto, Masanori, Tanaka, Tomonari, Yamane, Hideki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013574/
https://www.ncbi.nlm.nih.gov/pubmed/31947582
http://dx.doi.org/10.3390/ma13020377
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author Chen, Xuefei
Zhou, Lingling
Xu, Huaizhong
Yamamoto, Masaki
Shinoda, Masaya
Kishimoto, Masanori
Tanaka, Tomonari
Yamane, Hideki
author_facet Chen, Xuefei
Zhou, Lingling
Xu, Huaizhong
Yamamoto, Masaki
Shinoda, Masaya
Kishimoto, Masanori
Tanaka, Tomonari
Yamane, Hideki
author_sort Chen, Xuefei
collection PubMed
description Dehydrothermal (DHT) treatment was used to improve the properties of collagen casings because of its non-cytotoxicity. Understanding the effects of DHT treatment on the structure and mechanical properties of collagen films is beneficial to developing satisfying collagen casings. Herein, DHT treatment with various temperatures (85–145 °C) and timescales (1–7 days) were investigated. It was clarified that the chemical crosslinking covalent bond between collagen molecules was formed after the DHT treatment. Crosslinking density increased with increasing DHT treatment temperatures, contributing to the increase of tensile strength up to over three times of that of the untreated collagen film. The increased crosslinking density was also found when increasing the DHT treatment time, and the maximum was obtained in 3 days. Further DHT treatment time did not change the crosslinking density. The damage in the triple helix structure and the self-assembly of collagen molecules were observed from IR and SAXS. The extent of denaturation increased with increasing DHT treatment temperature and time, although the effect of the DHT treatment time on the denaturation was more moderate. When the DHT treatment temperature was as high as 145 °C or the DHT treatment time exceeded 5 days, serious denaturation occurs, leading to the deterioration of mechanical properties.
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spelling pubmed-70135742020-03-09 Effect of the Application of a Dehydrothermal Treatment on the Structure and the Mechanical Properties of Collagen Film Chen, Xuefei Zhou, Lingling Xu, Huaizhong Yamamoto, Masaki Shinoda, Masaya Kishimoto, Masanori Tanaka, Tomonari Yamane, Hideki Materials (Basel) Article Dehydrothermal (DHT) treatment was used to improve the properties of collagen casings because of its non-cytotoxicity. Understanding the effects of DHT treatment on the structure and mechanical properties of collagen films is beneficial to developing satisfying collagen casings. Herein, DHT treatment with various temperatures (85–145 °C) and timescales (1–7 days) were investigated. It was clarified that the chemical crosslinking covalent bond between collagen molecules was formed after the DHT treatment. Crosslinking density increased with increasing DHT treatment temperatures, contributing to the increase of tensile strength up to over three times of that of the untreated collagen film. The increased crosslinking density was also found when increasing the DHT treatment time, and the maximum was obtained in 3 days. Further DHT treatment time did not change the crosslinking density. The damage in the triple helix structure and the self-assembly of collagen molecules were observed from IR and SAXS. The extent of denaturation increased with increasing DHT treatment temperature and time, although the effect of the DHT treatment time on the denaturation was more moderate. When the DHT treatment temperature was as high as 145 °C or the DHT treatment time exceeded 5 days, serious denaturation occurs, leading to the deterioration of mechanical properties. MDPI 2020-01-14 /pmc/articles/PMC7013574/ /pubmed/31947582 http://dx.doi.org/10.3390/ma13020377 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Xuefei
Zhou, Lingling
Xu, Huaizhong
Yamamoto, Masaki
Shinoda, Masaya
Kishimoto, Masanori
Tanaka, Tomonari
Yamane, Hideki
Effect of the Application of a Dehydrothermal Treatment on the Structure and the Mechanical Properties of Collagen Film
title Effect of the Application of a Dehydrothermal Treatment on the Structure and the Mechanical Properties of Collagen Film
title_full Effect of the Application of a Dehydrothermal Treatment on the Structure and the Mechanical Properties of Collagen Film
title_fullStr Effect of the Application of a Dehydrothermal Treatment on the Structure and the Mechanical Properties of Collagen Film
title_full_unstemmed Effect of the Application of a Dehydrothermal Treatment on the Structure and the Mechanical Properties of Collagen Film
title_short Effect of the Application of a Dehydrothermal Treatment on the Structure and the Mechanical Properties of Collagen Film
title_sort effect of the application of a dehydrothermal treatment on the structure and the mechanical properties of collagen film
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013574/
https://www.ncbi.nlm.nih.gov/pubmed/31947582
http://dx.doi.org/10.3390/ma13020377
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