Cargando…
Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study
Six dentin adhesives were tested in vitro regarding their cytotoxicity on human fibroblasts. The adhesives Hybrid Bond, One-up Bond F Plus, AdheSE, Clearfil SE Bond, Optibond Solo Plus and Syntac were eluted with culture medium as single or sequentially applied adhesive part for 24 h. 75 Petri dishe...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013582/ https://www.ncbi.nlm.nih.gov/pubmed/31963535 http://dx.doi.org/10.3390/ma13020452 |
_version_ | 1783496436426473472 |
---|---|
author | Fröb, Luisa Rüttermann, Stefan Romanos, Georgios E. Herrmann, Eva Gerhardt-Szép, Susanne |
author_facet | Fröb, Luisa Rüttermann, Stefan Romanos, Georgios E. Herrmann, Eva Gerhardt-Szép, Susanne |
author_sort | Fröb, Luisa |
collection | PubMed |
description | Six dentin adhesives were tested in vitro regarding their cytotoxicity on human fibroblasts. The adhesives Hybrid Bond, One-up Bond F Plus, AdheSE, Clearfil SE Bond, Optibond Solo Plus and Syntac were eluted with culture medium as single or sequentially applied adhesive part for 24 h. 75 Petri dishes were produced per group. They were evaluated triangulated, comprising the quantitative evaluation (105 ones) to determine “viable”, “dead” and “debris” cells with the use of a cell-counter and the reactivity index was also identified based on the qualitative assessment (420 ones). One-up Bond F Plus, AdheSE and Clearfil SE Bond showed a statistical difference of viable cells to the cell control. For One-up Bond F Plus, statistically, differences compared to hybrid bond and Syntac were also found. All the adhesives except One-up Bond F Plus showed significant differences between single and sequentially applied adhesive part regarding the quantitative evaluation. The test material showed a moderate grade of cytotoxicity. As a result, a statistically significant difference of the cytotoxicity between the self-etch and etch-and-rinse adhesives cannot be demonstrated regarding the qualitative evaluation and the reactivity index, but the differences between sequentially applied and single applied components can be proved. |
format | Online Article Text |
id | pubmed-7013582 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70135822020-03-09 Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study Fröb, Luisa Rüttermann, Stefan Romanos, Georgios E. Herrmann, Eva Gerhardt-Szép, Susanne Materials (Basel) Article Six dentin adhesives were tested in vitro regarding their cytotoxicity on human fibroblasts. The adhesives Hybrid Bond, One-up Bond F Plus, AdheSE, Clearfil SE Bond, Optibond Solo Plus and Syntac were eluted with culture medium as single or sequentially applied adhesive part for 24 h. 75 Petri dishes were produced per group. They were evaluated triangulated, comprising the quantitative evaluation (105 ones) to determine “viable”, “dead” and “debris” cells with the use of a cell-counter and the reactivity index was also identified based on the qualitative assessment (420 ones). One-up Bond F Plus, AdheSE and Clearfil SE Bond showed a statistical difference of viable cells to the cell control. For One-up Bond F Plus, statistically, differences compared to hybrid bond and Syntac were also found. All the adhesives except One-up Bond F Plus showed significant differences between single and sequentially applied adhesive part regarding the quantitative evaluation. The test material showed a moderate grade of cytotoxicity. As a result, a statistically significant difference of the cytotoxicity between the self-etch and etch-and-rinse adhesives cannot be demonstrated regarding the qualitative evaluation and the reactivity index, but the differences between sequentially applied and single applied components can be proved. MDPI 2020-01-17 /pmc/articles/PMC7013582/ /pubmed/31963535 http://dx.doi.org/10.3390/ma13020452 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fröb, Luisa Rüttermann, Stefan Romanos, Georgios E. Herrmann, Eva Gerhardt-Szép, Susanne Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study |
title | Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study |
title_full | Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study |
title_fullStr | Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study |
title_full_unstemmed | Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study |
title_short | Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study |
title_sort | cytotoxicity of self-etch versus etch-and-rinse dentin adhesives: a screening study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013582/ https://www.ncbi.nlm.nih.gov/pubmed/31963535 http://dx.doi.org/10.3390/ma13020452 |
work_keys_str_mv | AT frobluisa cytotoxicityofselfetchversusetchandrinsedentinadhesivesascreeningstudy AT ruttermannstefan cytotoxicityofselfetchversusetchandrinsedentinadhesivesascreeningstudy AT romanosgeorgiose cytotoxicityofselfetchversusetchandrinsedentinadhesivesascreeningstudy AT herrmanneva cytotoxicityofselfetchversusetchandrinsedentinadhesivesascreeningstudy AT gerhardtszepsusanne cytotoxicityofselfetchversusetchandrinsedentinadhesivesascreeningstudy |