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Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study

Six dentin adhesives were tested in vitro regarding their cytotoxicity on human fibroblasts. The adhesives Hybrid Bond, One-up Bond F Plus, AdheSE, Clearfil SE Bond, Optibond Solo Plus and Syntac were eluted with culture medium as single or sequentially applied adhesive part for 24 h. 75 Petri dishe...

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Autores principales: Fröb, Luisa, Rüttermann, Stefan, Romanos, Georgios E., Herrmann, Eva, Gerhardt-Szép, Susanne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013582/
https://www.ncbi.nlm.nih.gov/pubmed/31963535
http://dx.doi.org/10.3390/ma13020452
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author Fröb, Luisa
Rüttermann, Stefan
Romanos, Georgios E.
Herrmann, Eva
Gerhardt-Szép, Susanne
author_facet Fröb, Luisa
Rüttermann, Stefan
Romanos, Georgios E.
Herrmann, Eva
Gerhardt-Szép, Susanne
author_sort Fröb, Luisa
collection PubMed
description Six dentin adhesives were tested in vitro regarding their cytotoxicity on human fibroblasts. The adhesives Hybrid Bond, One-up Bond F Plus, AdheSE, Clearfil SE Bond, Optibond Solo Plus and Syntac were eluted with culture medium as single or sequentially applied adhesive part for 24 h. 75 Petri dishes were produced per group. They were evaluated triangulated, comprising the quantitative evaluation (105 ones) to determine “viable”, “dead” and “debris” cells with the use of a cell-counter and the reactivity index was also identified based on the qualitative assessment (420 ones). One-up Bond F Plus, AdheSE and Clearfil SE Bond showed a statistical difference of viable cells to the cell control. For One-up Bond F Plus, statistically, differences compared to hybrid bond and Syntac were also found. All the adhesives except One-up Bond F Plus showed significant differences between single and sequentially applied adhesive part regarding the quantitative evaluation. The test material showed a moderate grade of cytotoxicity. As a result, a statistically significant difference of the cytotoxicity between the self-etch and etch-and-rinse adhesives cannot be demonstrated regarding the qualitative evaluation and the reactivity index, but the differences between sequentially applied and single applied components can be proved.
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spelling pubmed-70135822020-03-09 Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study Fröb, Luisa Rüttermann, Stefan Romanos, Georgios E. Herrmann, Eva Gerhardt-Szép, Susanne Materials (Basel) Article Six dentin adhesives were tested in vitro regarding their cytotoxicity on human fibroblasts. The adhesives Hybrid Bond, One-up Bond F Plus, AdheSE, Clearfil SE Bond, Optibond Solo Plus and Syntac were eluted with culture medium as single or sequentially applied adhesive part for 24 h. 75 Petri dishes were produced per group. They were evaluated triangulated, comprising the quantitative evaluation (105 ones) to determine “viable”, “dead” and “debris” cells with the use of a cell-counter and the reactivity index was also identified based on the qualitative assessment (420 ones). One-up Bond F Plus, AdheSE and Clearfil SE Bond showed a statistical difference of viable cells to the cell control. For One-up Bond F Plus, statistically, differences compared to hybrid bond and Syntac were also found. All the adhesives except One-up Bond F Plus showed significant differences between single and sequentially applied adhesive part regarding the quantitative evaluation. The test material showed a moderate grade of cytotoxicity. As a result, a statistically significant difference of the cytotoxicity between the self-etch and etch-and-rinse adhesives cannot be demonstrated regarding the qualitative evaluation and the reactivity index, but the differences between sequentially applied and single applied components can be proved. MDPI 2020-01-17 /pmc/articles/PMC7013582/ /pubmed/31963535 http://dx.doi.org/10.3390/ma13020452 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fröb, Luisa
Rüttermann, Stefan
Romanos, Georgios E.
Herrmann, Eva
Gerhardt-Szép, Susanne
Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study
title Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study
title_full Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study
title_fullStr Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study
title_full_unstemmed Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study
title_short Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study
title_sort cytotoxicity of self-etch versus etch-and-rinse dentin adhesives: a screening study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013582/
https://www.ncbi.nlm.nih.gov/pubmed/31963535
http://dx.doi.org/10.3390/ma13020452
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