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The Effects of TiO(2) Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines

There have been many studies on improving the efficacy of cisplatin and on identifying safe compounds that can overcome multi-drug resistance (MDR) acquired by cancer cells. Our previous research showed that polyethylene glycol-modified titanium dioxide nanoparticles (TiO(2) PEG NPs) affect cell mem...

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Autores principales: Salama, Basma, El-Sherbini, El-Said, El-Sayed, Gehad, El-Adl, Mohamed, Kanehira, Koki, Taniguchi, Akiyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013663/
https://www.ncbi.nlm.nih.gov/pubmed/31963452
http://dx.doi.org/10.3390/ijms21020605
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author Salama, Basma
El-Sherbini, El-Said
El-Sayed, Gehad
El-Adl, Mohamed
Kanehira, Koki
Taniguchi, Akiyoshi
author_facet Salama, Basma
El-Sherbini, El-Said
El-Sayed, Gehad
El-Adl, Mohamed
Kanehira, Koki
Taniguchi, Akiyoshi
author_sort Salama, Basma
collection PubMed
description There have been many studies on improving the efficacy of cisplatin and on identifying safe compounds that can overcome multi-drug resistance (MDR) acquired by cancer cells. Our previous research showed that polyethylene glycol-modified titanium dioxide nanoparticles (TiO(2) PEG NPs) affect cell membrane receptors, resulting in their aggregation, altered localization and downregulation. TiO(2) PEG NPs may affect P-glycoprotein (P-gp), a membrane efflux channel involved in MDR. In this study, we investigated the effect of TiO(2) PEG NPs on cisplatin cytotoxicity. We used HepG2 cells, which highly express P-gp and A431 cells, which show low expression of P-gp. The results showed that 10 µg/mL 100 nm TiO(2) PEG NPs increased intracellular cisplatin levels and cytotoxicity in HepG2 cells but not in A431 cells. TiO(2) PEG NPs treatment decreased the expression level of P-gp in HepG2 cells. Our findings indicate that TiO(2) PEG NPs enhance cisplatin cytotoxicity by down regulating P-gp and that TiO(2) PEG NPs are promising candidates for inhibiting P-gp and reversing drug resistance acquired by cancer cells.
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spelling pubmed-70136632020-03-09 The Effects of TiO(2) Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines Salama, Basma El-Sherbini, El-Said El-Sayed, Gehad El-Adl, Mohamed Kanehira, Koki Taniguchi, Akiyoshi Int J Mol Sci Article There have been many studies on improving the efficacy of cisplatin and on identifying safe compounds that can overcome multi-drug resistance (MDR) acquired by cancer cells. Our previous research showed that polyethylene glycol-modified titanium dioxide nanoparticles (TiO(2) PEG NPs) affect cell membrane receptors, resulting in their aggregation, altered localization and downregulation. TiO(2) PEG NPs may affect P-glycoprotein (P-gp), a membrane efflux channel involved in MDR. In this study, we investigated the effect of TiO(2) PEG NPs on cisplatin cytotoxicity. We used HepG2 cells, which highly express P-gp and A431 cells, which show low expression of P-gp. The results showed that 10 µg/mL 100 nm TiO(2) PEG NPs increased intracellular cisplatin levels and cytotoxicity in HepG2 cells but not in A431 cells. TiO(2) PEG NPs treatment decreased the expression level of P-gp in HepG2 cells. Our findings indicate that TiO(2) PEG NPs enhance cisplatin cytotoxicity by down regulating P-gp and that TiO(2) PEG NPs are promising candidates for inhibiting P-gp and reversing drug resistance acquired by cancer cells. MDPI 2020-01-17 /pmc/articles/PMC7013663/ /pubmed/31963452 http://dx.doi.org/10.3390/ijms21020605 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Salama, Basma
El-Sherbini, El-Said
El-Sayed, Gehad
El-Adl, Mohamed
Kanehira, Koki
Taniguchi, Akiyoshi
The Effects of TiO(2) Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines
title The Effects of TiO(2) Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines
title_full The Effects of TiO(2) Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines
title_fullStr The Effects of TiO(2) Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines
title_full_unstemmed The Effects of TiO(2) Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines
title_short The Effects of TiO(2) Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines
title_sort effects of tio(2) nanoparticles on cisplatin cytotoxicity in cancer cell lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013663/
https://www.ncbi.nlm.nih.gov/pubmed/31963452
http://dx.doi.org/10.3390/ijms21020605
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