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The Effects of TiO(2) Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines
There have been many studies on improving the efficacy of cisplatin and on identifying safe compounds that can overcome multi-drug resistance (MDR) acquired by cancer cells. Our previous research showed that polyethylene glycol-modified titanium dioxide nanoparticles (TiO(2) PEG NPs) affect cell mem...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013663/ https://www.ncbi.nlm.nih.gov/pubmed/31963452 http://dx.doi.org/10.3390/ijms21020605 |
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author | Salama, Basma El-Sherbini, El-Said El-Sayed, Gehad El-Adl, Mohamed Kanehira, Koki Taniguchi, Akiyoshi |
author_facet | Salama, Basma El-Sherbini, El-Said El-Sayed, Gehad El-Adl, Mohamed Kanehira, Koki Taniguchi, Akiyoshi |
author_sort | Salama, Basma |
collection | PubMed |
description | There have been many studies on improving the efficacy of cisplatin and on identifying safe compounds that can overcome multi-drug resistance (MDR) acquired by cancer cells. Our previous research showed that polyethylene glycol-modified titanium dioxide nanoparticles (TiO(2) PEG NPs) affect cell membrane receptors, resulting in their aggregation, altered localization and downregulation. TiO(2) PEG NPs may affect P-glycoprotein (P-gp), a membrane efflux channel involved in MDR. In this study, we investigated the effect of TiO(2) PEG NPs on cisplatin cytotoxicity. We used HepG2 cells, which highly express P-gp and A431 cells, which show low expression of P-gp. The results showed that 10 µg/mL 100 nm TiO(2) PEG NPs increased intracellular cisplatin levels and cytotoxicity in HepG2 cells but not in A431 cells. TiO(2) PEG NPs treatment decreased the expression level of P-gp in HepG2 cells. Our findings indicate that TiO(2) PEG NPs enhance cisplatin cytotoxicity by down regulating P-gp and that TiO(2) PEG NPs are promising candidates for inhibiting P-gp and reversing drug resistance acquired by cancer cells. |
format | Online Article Text |
id | pubmed-7013663 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70136632020-03-09 The Effects of TiO(2) Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines Salama, Basma El-Sherbini, El-Said El-Sayed, Gehad El-Adl, Mohamed Kanehira, Koki Taniguchi, Akiyoshi Int J Mol Sci Article There have been many studies on improving the efficacy of cisplatin and on identifying safe compounds that can overcome multi-drug resistance (MDR) acquired by cancer cells. Our previous research showed that polyethylene glycol-modified titanium dioxide nanoparticles (TiO(2) PEG NPs) affect cell membrane receptors, resulting in their aggregation, altered localization and downregulation. TiO(2) PEG NPs may affect P-glycoprotein (P-gp), a membrane efflux channel involved in MDR. In this study, we investigated the effect of TiO(2) PEG NPs on cisplatin cytotoxicity. We used HepG2 cells, which highly express P-gp and A431 cells, which show low expression of P-gp. The results showed that 10 µg/mL 100 nm TiO(2) PEG NPs increased intracellular cisplatin levels and cytotoxicity in HepG2 cells but not in A431 cells. TiO(2) PEG NPs treatment decreased the expression level of P-gp in HepG2 cells. Our findings indicate that TiO(2) PEG NPs enhance cisplatin cytotoxicity by down regulating P-gp and that TiO(2) PEG NPs are promising candidates for inhibiting P-gp and reversing drug resistance acquired by cancer cells. MDPI 2020-01-17 /pmc/articles/PMC7013663/ /pubmed/31963452 http://dx.doi.org/10.3390/ijms21020605 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Salama, Basma El-Sherbini, El-Said El-Sayed, Gehad El-Adl, Mohamed Kanehira, Koki Taniguchi, Akiyoshi The Effects of TiO(2) Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines |
title | The Effects of TiO(2) Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines |
title_full | The Effects of TiO(2) Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines |
title_fullStr | The Effects of TiO(2) Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines |
title_full_unstemmed | The Effects of TiO(2) Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines |
title_short | The Effects of TiO(2) Nanoparticles on Cisplatin Cytotoxicity in Cancer Cell Lines |
title_sort | effects of tio(2) nanoparticles on cisplatin cytotoxicity in cancer cell lines |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013663/ https://www.ncbi.nlm.nih.gov/pubmed/31963452 http://dx.doi.org/10.3390/ijms21020605 |
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