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Proteomic Analysis of Irradiation with Millimeter Waves on Soybean Growth under Flooding Conditions

Improving soybean growth and tolerance under environmental stress is crucial for sustainable development. Millimeter waves are a radio-frequency band with a wavelength range of 1–10 mm that has dynamic effects on organisms. To investigate the potential effects of millimeter-waves irradiation on soyb...

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Autores principales: Zhong, Zhuoheng, Furuya, Takashi, Ueno, Kimitaka, Yamaguchi, Hisateru, Hitachi, Keisuke, Tsuchida, Kunihiro, Tani, Masahiko, Tian, Jingkui, Komatsu, Setsuko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013696/
https://www.ncbi.nlm.nih.gov/pubmed/31940953
http://dx.doi.org/10.3390/ijms21020486
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author Zhong, Zhuoheng
Furuya, Takashi
Ueno, Kimitaka
Yamaguchi, Hisateru
Hitachi, Keisuke
Tsuchida, Kunihiro
Tani, Masahiko
Tian, Jingkui
Komatsu, Setsuko
author_facet Zhong, Zhuoheng
Furuya, Takashi
Ueno, Kimitaka
Yamaguchi, Hisateru
Hitachi, Keisuke
Tsuchida, Kunihiro
Tani, Masahiko
Tian, Jingkui
Komatsu, Setsuko
author_sort Zhong, Zhuoheng
collection PubMed
description Improving soybean growth and tolerance under environmental stress is crucial for sustainable development. Millimeter waves are a radio-frequency band with a wavelength range of 1–10 mm that has dynamic effects on organisms. To investigate the potential effects of millimeter-waves irradiation on soybean seedlings, morphological and proteomic analyses were performed. Millimeter-waves irradiation improved the growth of roots/hypocotyl and the tolerance of soybean to flooding stress. Proteomic analysis indicated that the irradiated soybean seedlings recovered under oxidative stress during growth, whereas proteins related to glycolysis and ascorbate/glutathione metabolism were not affected. Immunoblot analysis confirmed the promotive effect of millimeter waves to glycolysis- and redox-related pathways under flooding conditions. Sugar metabolism was suppressed under flooding in unirradiated soybean seedlings, whereas it was activated in the irradiated ones, especially trehalose synthesis. These results suggest that millimeter-waves irradiation on soybean seeds promotes the recovery of soybean seedlings under oxidative stress, which positively regulates soybean growth through the regulation of glycolysis and redox related pathways.
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spelling pubmed-70136962020-03-09 Proteomic Analysis of Irradiation with Millimeter Waves on Soybean Growth under Flooding Conditions Zhong, Zhuoheng Furuya, Takashi Ueno, Kimitaka Yamaguchi, Hisateru Hitachi, Keisuke Tsuchida, Kunihiro Tani, Masahiko Tian, Jingkui Komatsu, Setsuko Int J Mol Sci Article Improving soybean growth and tolerance under environmental stress is crucial for sustainable development. Millimeter waves are a radio-frequency band with a wavelength range of 1–10 mm that has dynamic effects on organisms. To investigate the potential effects of millimeter-waves irradiation on soybean seedlings, morphological and proteomic analyses were performed. Millimeter-waves irradiation improved the growth of roots/hypocotyl and the tolerance of soybean to flooding stress. Proteomic analysis indicated that the irradiated soybean seedlings recovered under oxidative stress during growth, whereas proteins related to glycolysis and ascorbate/glutathione metabolism were not affected. Immunoblot analysis confirmed the promotive effect of millimeter waves to glycolysis- and redox-related pathways under flooding conditions. Sugar metabolism was suppressed under flooding in unirradiated soybean seedlings, whereas it was activated in the irradiated ones, especially trehalose synthesis. These results suggest that millimeter-waves irradiation on soybean seeds promotes the recovery of soybean seedlings under oxidative stress, which positively regulates soybean growth through the regulation of glycolysis and redox related pathways. MDPI 2020-01-12 /pmc/articles/PMC7013696/ /pubmed/31940953 http://dx.doi.org/10.3390/ijms21020486 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhong, Zhuoheng
Furuya, Takashi
Ueno, Kimitaka
Yamaguchi, Hisateru
Hitachi, Keisuke
Tsuchida, Kunihiro
Tani, Masahiko
Tian, Jingkui
Komatsu, Setsuko
Proteomic Analysis of Irradiation with Millimeter Waves on Soybean Growth under Flooding Conditions
title Proteomic Analysis of Irradiation with Millimeter Waves on Soybean Growth under Flooding Conditions
title_full Proteomic Analysis of Irradiation with Millimeter Waves on Soybean Growth under Flooding Conditions
title_fullStr Proteomic Analysis of Irradiation with Millimeter Waves on Soybean Growth under Flooding Conditions
title_full_unstemmed Proteomic Analysis of Irradiation with Millimeter Waves on Soybean Growth under Flooding Conditions
title_short Proteomic Analysis of Irradiation with Millimeter Waves on Soybean Growth under Flooding Conditions
title_sort proteomic analysis of irradiation with millimeter waves on soybean growth under flooding conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013696/
https://www.ncbi.nlm.nih.gov/pubmed/31940953
http://dx.doi.org/10.3390/ijms21020486
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