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Rapid Cellular Perception of Gravitational Forces in Human Jurkat T Cells and Transduction into Gene Expression Regulation

Cellular processes are influenced in many ways by changes in gravitational force. In previous studies, we were able to demonstrate, in various cellular systems and research platforms that reactions and adaptation processes occur very rapidly after the onset of altered gravity. In this study we syste...

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Autores principales: Thiel, Cora Sandra, Christoffel, Swantje, Tauber, Svantje, Vahlensieck, Christian, de Zélicourt, Diane, Layer, Liliana E., Lauber, Beatrice, Polzer, Jennifer, Ullrich, Oliver
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013750/
https://www.ncbi.nlm.nih.gov/pubmed/31947583
http://dx.doi.org/10.3390/ijms21020514
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author Thiel, Cora Sandra
Christoffel, Swantje
Tauber, Svantje
Vahlensieck, Christian
de Zélicourt, Diane
Layer, Liliana E.
Lauber, Beatrice
Polzer, Jennifer
Ullrich, Oliver
author_facet Thiel, Cora Sandra
Christoffel, Swantje
Tauber, Svantje
Vahlensieck, Christian
de Zélicourt, Diane
Layer, Liliana E.
Lauber, Beatrice
Polzer, Jennifer
Ullrich, Oliver
author_sort Thiel, Cora Sandra
collection PubMed
description Cellular processes are influenced in many ways by changes in gravitational force. In previous studies, we were able to demonstrate, in various cellular systems and research platforms that reactions and adaptation processes occur very rapidly after the onset of altered gravity. In this study we systematically compared differentially expressed gene transcript clusters (TCs) in human Jurkat T cells in microgravity provided by a suborbital ballistic rocket with vector-averaged gravity (vag) provided by a 2D clinostat. Additionally, we included 9× g centrifuge experiments and rigorous controls for excluding other factors of influence than gravity. We found that 11 TCs were significantly altered in 5 min of flight-induced and vector-averaged gravity. Among the annotated clusters were G3BP1, KPNB1, NUDT3, SFT2D2, and POMK. Our results revealed that less than 1% of all examined TCs show the same response in vag and flight-induced microgravity, while 38% of differentially regulated TCs identified during the hypergravity phase of the suborbital ballistic rocket flight could be verified with a 9× g ground centrifuge. In the 2D clinostat system, doing one full rotation per second, vector effects of the gravitational force are only nullified if the sensing mechanism requires 1 s or longer. Due to the fact that vag with an integration period of 1 s was not able to reproduce the results obtained in flight-induced microgravity, we conclude that the initial trigger of gene expression response to microgravity requires less than 1 s reaction time. Additionally, we discovered extensive gene expression differences caused by simple handling of the cell suspension in control experiments, which underlines the need for rigorous standardization regarding mechanical forces during cell culture experiments in general.
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spelling pubmed-70137502020-03-09 Rapid Cellular Perception of Gravitational Forces in Human Jurkat T Cells and Transduction into Gene Expression Regulation Thiel, Cora Sandra Christoffel, Swantje Tauber, Svantje Vahlensieck, Christian de Zélicourt, Diane Layer, Liliana E. Lauber, Beatrice Polzer, Jennifer Ullrich, Oliver Int J Mol Sci Article Cellular processes are influenced in many ways by changes in gravitational force. In previous studies, we were able to demonstrate, in various cellular systems and research platforms that reactions and adaptation processes occur very rapidly after the onset of altered gravity. In this study we systematically compared differentially expressed gene transcript clusters (TCs) in human Jurkat T cells in microgravity provided by a suborbital ballistic rocket with vector-averaged gravity (vag) provided by a 2D clinostat. Additionally, we included 9× g centrifuge experiments and rigorous controls for excluding other factors of influence than gravity. We found that 11 TCs were significantly altered in 5 min of flight-induced and vector-averaged gravity. Among the annotated clusters were G3BP1, KPNB1, NUDT3, SFT2D2, and POMK. Our results revealed that less than 1% of all examined TCs show the same response in vag and flight-induced microgravity, while 38% of differentially regulated TCs identified during the hypergravity phase of the suborbital ballistic rocket flight could be verified with a 9× g ground centrifuge. In the 2D clinostat system, doing one full rotation per second, vector effects of the gravitational force are only nullified if the sensing mechanism requires 1 s or longer. Due to the fact that vag with an integration period of 1 s was not able to reproduce the results obtained in flight-induced microgravity, we conclude that the initial trigger of gene expression response to microgravity requires less than 1 s reaction time. Additionally, we discovered extensive gene expression differences caused by simple handling of the cell suspension in control experiments, which underlines the need for rigorous standardization regarding mechanical forces during cell culture experiments in general. MDPI 2020-01-14 /pmc/articles/PMC7013750/ /pubmed/31947583 http://dx.doi.org/10.3390/ijms21020514 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Thiel, Cora Sandra
Christoffel, Swantje
Tauber, Svantje
Vahlensieck, Christian
de Zélicourt, Diane
Layer, Liliana E.
Lauber, Beatrice
Polzer, Jennifer
Ullrich, Oliver
Rapid Cellular Perception of Gravitational Forces in Human Jurkat T Cells and Transduction into Gene Expression Regulation
title Rapid Cellular Perception of Gravitational Forces in Human Jurkat T Cells and Transduction into Gene Expression Regulation
title_full Rapid Cellular Perception of Gravitational Forces in Human Jurkat T Cells and Transduction into Gene Expression Regulation
title_fullStr Rapid Cellular Perception of Gravitational Forces in Human Jurkat T Cells and Transduction into Gene Expression Regulation
title_full_unstemmed Rapid Cellular Perception of Gravitational Forces in Human Jurkat T Cells and Transduction into Gene Expression Regulation
title_short Rapid Cellular Perception of Gravitational Forces in Human Jurkat T Cells and Transduction into Gene Expression Regulation
title_sort rapid cellular perception of gravitational forces in human jurkat t cells and transduction into gene expression regulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013750/
https://www.ncbi.nlm.nih.gov/pubmed/31947583
http://dx.doi.org/10.3390/ijms21020514
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