Cargando…

The Impact of IPTG Induction on Plasmid Stability and Heterologous Protein Expression by Escherichia coli Biofilms

This work assesses the effect of chemical induction with isopropyl β-D-1-thiogalactopyranoside (IPTG) on the expression of enhanced green fluorescent protein (eGFP) by planktonic and biofilm cells of Escherichia coli JM109(DE3) transformed with a plasmid containing a T7 promoter. It was shown that i...

Descripción completa

Detalles Bibliográficos
Autores principales:	Gomes, Luciana, Monteiro, Gabriel, Mergulhão, Filipe
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013871/ https://www.ncbi.nlm.nih.gov/pubmed/31963160 http://dx.doi.org/10.3390/ijms21020576

Ejemplares similares

Using Lactobacilli to Fight Escherichia coli and Staphylococcus aureus Biofilms on Urinary Tract Devices
por: Carvalho, Fábio M., et al.
Publicado: (2021)

Effect of Lactobacillus plantarum Biofilms on the Adhesion of Escherichia coli to Urinary Tract Devices
por: Carvalho, Fábio M., et al.
Publicado: (2021)

Efficacy of A Poly(MeOEGMA) Brush on the Prevention of Escherichia coli Biofilm Formation and Susceptibility
por: Alves, Patrícia, et al.
Publicado: (2020)

Hydrodynamic Effects on Biofilm Development and Recombinant Protein Expression
por: Soares, Alexandra, et al.
Publicado: (2022)

Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid
por: Naorem, Santa S., et al.
Publicado: (2018)

Surface conditioning with Escherichia coli cell wall components can reduce biofilm formation by decreasing initial adhesion
por: Gomes, Luciana C., et al.
Publicado: (2017)

SEM Analysis of Surface Impact on Biofilm Antibiotic Treatment
por: Gomes, Luciana Calheiros, et al.
Publicado: (2017)

Development of inducer-free expression plasmids based on IPTG-inducible promoters for Bacillus subtilis
por: Tran, Dinh Thi Minh, et al.
Publicado: (2017)

Evaluation of pre-induction temperature, cell growth at induction and IPTG concentration on the expression of a leptospiral protein in E. coli using shaking flasks and microbioreactor
por: Larentis, Ariane Leites, et al.
Publicado: (2014)

Direct measurements of IPTG enable analysis of the induction behavior of E. coli in high cell density cultures
por: Fernández-Castané, Alfred, et al.
Publicado: (2012)

Mechanistic aspects of IPTG (isopropylthio-β-galactoside) transport across the cytoplasmic membrane of Escherichia coli—a rate limiting step in the induction of recombinant protein expression
por: Simas, Rodrigo G, et al.
Publicado: (2023)

Recombinant protein expression in biofilms
por: Soares, Alexandra, et al.
Publicado: (2019)

An IPTG Inducible Conditional Expression System for Mycobacteria
por: Ravishankar, Sudha, et al.
Publicado: (2015)

Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway
por: Dvorak, Pavel, et al.
Publicado: (2015)

Comparing the Recombinant Protein Production Potential of Planktonic and Biofilm Cells
por: Soares, Alexandra, et al.
Publicado: (2018)

Improvement of Pseudoalteromonas haloplanktis TAC125 as a Cell Factory: IPTG-Inducible Plasmid Construction and Strain Engineering
por: Colarusso, Andrea, et al.
Publicado: (2020)

A Selection of Platforms to Evaluate Surface Adhesion and Biofilm Formation in Controlled Hydrodynamic Conditions
por: Gomes, Luciana C., et al.
Publicado: (2021)

Increased Intraspecies Diversity in Escherichia coli Biofilms Promotes Cellular Growth at the Expense of Matrix Production
por: Azevedo, Andreia S., et al.
Publicado: (2020)

Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system
por: Wandrey, Georg, et al.
Publicado: (2016)

Challenges in the Heterologous Production of Furanocoumarins in Escherichia coli
por: Rodrigues, Joana L., et al.
Publicado: (2022)

Effect of Temperature, pH and Plasmids on In Vitro Biofilm Formation in Escherichia coli
por: Mathlouthi, A., et al.
Publicado: (2018)

Optimization of expression JTAT protein with emphasis on transformation efficiency and IPTG concentration
por: Margawati, Endang Tri, et al.
Publicado: (2017)

Development of the Mammalian Expression Vector System that can be Induced by IPTG and/or Lactose
por: Myung, Seung-Hyun, et al.
Publicado: (2020)

High-level production of membrane proteins in E. coli BL21(DE3) by omitting the inducer IPTG
por: Zhang, Zhe, et al.
Publicado: (2015)

Delineating Bacteriostatic and Bactericidal Targets in Mycobacteria Using IPTG Inducible Antisense Expression
por: Kaur, Parvinder, et al.
Publicado: (2009)

Colicin E2 expression in Escherichia coli biofilms: Induction and regulation revisited
por: Bayramoglu-Güven, Bihter, et al.
Publicado: (2022)

The Use of Probiotics to Fight Biofilms in Medical Devices: A Systematic Review and Meta-Analysis
por: Carvalho, Fábio M., et al.
Publicado: (2020)

Generation of Small Colony Variants in Biofilms by Escherichia coli Harboring a Conjugative F Plasmid
por: Tashiro, Yosuke, et al.
Publicado: (2017)

Biofilm Localization in the Vertical Wall of Shaking 96-Well Plates
por: Gomes, Luciana C., et al.
Publicado: (2014)

Advances on Bacterial and Fungal Biofilms for the Production of Added-Value Compounds
por: Carvalho, Fábio M., et al.
Publicado: (2022)

Generation and Evaluation of an IPTG-Regulated Version of Vav-Gene Promoter for Mouse Transgenesis
por: Grespi, Francesca, et al.
Publicado: (2011)

Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3
por: Al-Allaf, Faisal A., et al.
Publicado: (2012)

Use of Probiotics to Control Biofilm Formation in Food Industries
por: Tomé, Andreia R., et al.
Publicado: (2023)

Heterologous expression of a thermophilic diacylglycerol acyltransferase triggers triglyceride accumulation in Escherichia coli
por: Lázaro, Beatriz, et al.
Publicado: (2017)

Enhanced Biofilm Formation and Membrane Vesicle Release by Escherichia coli Expressing a Commonly Occurring Plasmid Gene, kil
por: Nakao, Ryoma, et al.
Publicado: (2018)

High-Yield Expression of Heterologous [FeFe] Hydrogenases in Escherichia coli
por: Kuchenreuther, Jon M., et al.
Publicado: (2010)

A self-inducible heterologous protein expression system in Escherichia coli
por: Briand, L., et al.
Publicado: (2016)

Heterologous Expression of Mersacidin in Escherichia coli Elucidates the Mode of Leader Processing
por: Viel, Jakob H., et al.
Publicado: (2021)

Role of Chromosome- and/or Plasmid-Located bla(NDM) on the Carbapenem Resistance and the Gene Stability in Escherichia coli
por: Sakamoto, Noriko, et al.
Publicado: (2022)

Performance of Graphene/Polydimethylsiloxane Surfaces against S. aureus and P. aeruginosa Single- and Dual-Species Biofilms
por: Oliveira, Isabel M., et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_c0mgq61mqhpd0updulkrdeif68