Cargando…

Small RNA and mRNA Sequencing Reveal the Roles of microRNAs Involved in Pomegranate Female Sterility

Female sterility is a key factor restricting plant reproduction. Our previous studies have revealed that pomegranate female sterility mainly arose from the abnormality of ovule development. MicroRNAs (miRNAs) play important roles in ovule development. However, little is known about the roles of miRN...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Lina, Luo, Xiang, Yang, Xuanwen, Jing, Dan, Xia, Xiaocong, Li, Haoxian, Poudel, Krishna, Cao, Shangyin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013964/
https://www.ncbi.nlm.nih.gov/pubmed/31952315
http://dx.doi.org/10.3390/ijms21020558
_version_ 1783496519896268800
author Chen, Lina
Luo, Xiang
Yang, Xuanwen
Jing, Dan
Xia, Xiaocong
Li, Haoxian
Poudel, Krishna
Cao, Shangyin
author_facet Chen, Lina
Luo, Xiang
Yang, Xuanwen
Jing, Dan
Xia, Xiaocong
Li, Haoxian
Poudel, Krishna
Cao, Shangyin
author_sort Chen, Lina
collection PubMed
description Female sterility is a key factor restricting plant reproduction. Our previous studies have revealed that pomegranate female sterility mainly arose from the abnormality of ovule development. MicroRNAs (miRNAs) play important roles in ovule development. However, little is known about the roles of miRNAs in female sterility. In this study, a combined high-throughput sequencing approach was used to investigate the miRNAs and their targeted transcripts involved in female development. A total of 103 conserved and 58 novel miRNAs were identified. Comparative profiling indicated that the expression of 43 known miRNAs and 14 novel miRNAs were differentially expressed between functional male flowers (FMFs) and bisexual flowers (BFs), 30 known miRNAs and nine novel miRNAs showed significant differences among different stages of BFs, and 20 known miRNAs and 18 novel miRNAs exhibited remarkable expression differences among different stages of FMFs. Gene ontology (GO) analyses of 144 predicted targets of differentially expressed miRNAs indicated that the “reproduction process” and “floral whorl development” processes were significantly enriched. The miRNA–mRNA interaction analyses revealed six pairs of candidate miRNAs and their targets associated with female sterility. Interestingly, pg-miR166a-3p was accumulated, whereas its predicted targets (Gglean012177.1 and Gglean013966.1) were repressed in functional male flowers (FMFs), and the interaction between pg-miR166a-3p and its targets (Gglean012177.1 and Gglean013966.1) were confirmed by transient assay. A. thaliana transformed with 35S-pre-pg-miR166a-3p verified the role of pg-miR166a-3p in ovule development, which indicated pg-miR166a-3p’s potential role in pomegranate female sterility. The results provide new insights into molecular mechanisms underlying the female sterility at the miRNA level.
format Online
Article
Text
id pubmed-7013964
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70139642020-03-09 Small RNA and mRNA Sequencing Reveal the Roles of microRNAs Involved in Pomegranate Female Sterility Chen, Lina Luo, Xiang Yang, Xuanwen Jing, Dan Xia, Xiaocong Li, Haoxian Poudel, Krishna Cao, Shangyin Int J Mol Sci Article Female sterility is a key factor restricting plant reproduction. Our previous studies have revealed that pomegranate female sterility mainly arose from the abnormality of ovule development. MicroRNAs (miRNAs) play important roles in ovule development. However, little is known about the roles of miRNAs in female sterility. In this study, a combined high-throughput sequencing approach was used to investigate the miRNAs and their targeted transcripts involved in female development. A total of 103 conserved and 58 novel miRNAs were identified. Comparative profiling indicated that the expression of 43 known miRNAs and 14 novel miRNAs were differentially expressed between functional male flowers (FMFs) and bisexual flowers (BFs), 30 known miRNAs and nine novel miRNAs showed significant differences among different stages of BFs, and 20 known miRNAs and 18 novel miRNAs exhibited remarkable expression differences among different stages of FMFs. Gene ontology (GO) analyses of 144 predicted targets of differentially expressed miRNAs indicated that the “reproduction process” and “floral whorl development” processes were significantly enriched. The miRNA–mRNA interaction analyses revealed six pairs of candidate miRNAs and their targets associated with female sterility. Interestingly, pg-miR166a-3p was accumulated, whereas its predicted targets (Gglean012177.1 and Gglean013966.1) were repressed in functional male flowers (FMFs), and the interaction between pg-miR166a-3p and its targets (Gglean012177.1 and Gglean013966.1) were confirmed by transient assay. A. thaliana transformed with 35S-pre-pg-miR166a-3p verified the role of pg-miR166a-3p in ovule development, which indicated pg-miR166a-3p’s potential role in pomegranate female sterility. The results provide new insights into molecular mechanisms underlying the female sterility at the miRNA level. MDPI 2020-01-15 /pmc/articles/PMC7013964/ /pubmed/31952315 http://dx.doi.org/10.3390/ijms21020558 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Lina
Luo, Xiang
Yang, Xuanwen
Jing, Dan
Xia, Xiaocong
Li, Haoxian
Poudel, Krishna
Cao, Shangyin
Small RNA and mRNA Sequencing Reveal the Roles of microRNAs Involved in Pomegranate Female Sterility
title Small RNA and mRNA Sequencing Reveal the Roles of microRNAs Involved in Pomegranate Female Sterility
title_full Small RNA and mRNA Sequencing Reveal the Roles of microRNAs Involved in Pomegranate Female Sterility
title_fullStr Small RNA and mRNA Sequencing Reveal the Roles of microRNAs Involved in Pomegranate Female Sterility
title_full_unstemmed Small RNA and mRNA Sequencing Reveal the Roles of microRNAs Involved in Pomegranate Female Sterility
title_short Small RNA and mRNA Sequencing Reveal the Roles of microRNAs Involved in Pomegranate Female Sterility
title_sort small rna and mrna sequencing reveal the roles of micrornas involved in pomegranate female sterility
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013964/
https://www.ncbi.nlm.nih.gov/pubmed/31952315
http://dx.doi.org/10.3390/ijms21020558
work_keys_str_mv AT chenlina smallrnaandmrnasequencingrevealtherolesofmicrornasinvolvedinpomegranatefemalesterility
AT luoxiang smallrnaandmrnasequencingrevealtherolesofmicrornasinvolvedinpomegranatefemalesterility
AT yangxuanwen smallrnaandmrnasequencingrevealtherolesofmicrornasinvolvedinpomegranatefemalesterility
AT jingdan smallrnaandmrnasequencingrevealtherolesofmicrornasinvolvedinpomegranatefemalesterility
AT xiaxiaocong smallrnaandmrnasequencingrevealtherolesofmicrornasinvolvedinpomegranatefemalesterility
AT lihaoxian smallrnaandmrnasequencingrevealtherolesofmicrornasinvolvedinpomegranatefemalesterility
AT poudelkrishna smallrnaandmrnasequencingrevealtherolesofmicrornasinvolvedinpomegranatefemalesterility
AT caoshangyin smallrnaandmrnasequencingrevealtherolesofmicrornasinvolvedinpomegranatefemalesterility