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Diffusion Reflection Measurements of Antibodies Conjugated to Gold Nanoparticles as a Method to Identify Cutaneous Squamous Cell Carcinoma Borders

Diffusion reflectance spectroscopy measurements targeted with gold nanoparticles (GNPs) can identify residual cutaneous squamous cell carcinoma (SCC) in excision borders. Human SCC specimens were stained with hematoxylin and eosin to identify tumor borders, and reflected onto an unstained deparaffin...

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Autores principales: Olshinka, Asaf, Ad-El, Dean, Didkovski, Elena, Weiss, Shirel, Ankri, Rinat, Goldenberg-Cohen, Nitza, Fixler, Dror
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7014005/
https://www.ncbi.nlm.nih.gov/pubmed/31963462
http://dx.doi.org/10.3390/ma13020447
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author Olshinka, Asaf
Ad-El, Dean
Didkovski, Elena
Weiss, Shirel
Ankri, Rinat
Goldenberg-Cohen, Nitza
Fixler, Dror
author_facet Olshinka, Asaf
Ad-El, Dean
Didkovski, Elena
Weiss, Shirel
Ankri, Rinat
Goldenberg-Cohen, Nitza
Fixler, Dror
author_sort Olshinka, Asaf
collection PubMed
description Diffusion reflectance spectroscopy measurements targeted with gold nanoparticles (GNPs) can identify residual cutaneous squamous cell carcinoma (SCC) in excision borders. Human SCC specimens were stained with hematoxylin and eosin to identify tumor borders, and reflected onto an unstained deparaffinized section. Diffusion reflection of three sites (normal and SCC) were measured before and after GNPs targeting. Hyperspectral imaging showed a mean of 2.5 sites with tumor per specimen and 1.2 tumor-free (p < 0.05, t-test). GNPs were detected in 25/30 tumor sites (sensitivity 83.3%, false-negative rate 16.6%) and 12/30 non-tumor sites (specificity 60%, false-positive rate 40%). This study verifies the use of nanotechnology in identifying SCC tumor margins. Diffusion reflection scanning has high sensitivity for detecting the residual tumor.
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spelling pubmed-70140052020-03-09 Diffusion Reflection Measurements of Antibodies Conjugated to Gold Nanoparticles as a Method to Identify Cutaneous Squamous Cell Carcinoma Borders Olshinka, Asaf Ad-El, Dean Didkovski, Elena Weiss, Shirel Ankri, Rinat Goldenberg-Cohen, Nitza Fixler, Dror Materials (Basel) Article Diffusion reflectance spectroscopy measurements targeted with gold nanoparticles (GNPs) can identify residual cutaneous squamous cell carcinoma (SCC) in excision borders. Human SCC specimens were stained with hematoxylin and eosin to identify tumor borders, and reflected onto an unstained deparaffinized section. Diffusion reflection of three sites (normal and SCC) were measured before and after GNPs targeting. Hyperspectral imaging showed a mean of 2.5 sites with tumor per specimen and 1.2 tumor-free (p < 0.05, t-test). GNPs were detected in 25/30 tumor sites (sensitivity 83.3%, false-negative rate 16.6%) and 12/30 non-tumor sites (specificity 60%, false-positive rate 40%). This study verifies the use of nanotechnology in identifying SCC tumor margins. Diffusion reflection scanning has high sensitivity for detecting the residual tumor. MDPI 2020-01-17 /pmc/articles/PMC7014005/ /pubmed/31963462 http://dx.doi.org/10.3390/ma13020447 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Olshinka, Asaf
Ad-El, Dean
Didkovski, Elena
Weiss, Shirel
Ankri, Rinat
Goldenberg-Cohen, Nitza
Fixler, Dror
Diffusion Reflection Measurements of Antibodies Conjugated to Gold Nanoparticles as a Method to Identify Cutaneous Squamous Cell Carcinoma Borders
title Diffusion Reflection Measurements of Antibodies Conjugated to Gold Nanoparticles as a Method to Identify Cutaneous Squamous Cell Carcinoma Borders
title_full Diffusion Reflection Measurements of Antibodies Conjugated to Gold Nanoparticles as a Method to Identify Cutaneous Squamous Cell Carcinoma Borders
title_fullStr Diffusion Reflection Measurements of Antibodies Conjugated to Gold Nanoparticles as a Method to Identify Cutaneous Squamous Cell Carcinoma Borders
title_full_unstemmed Diffusion Reflection Measurements of Antibodies Conjugated to Gold Nanoparticles as a Method to Identify Cutaneous Squamous Cell Carcinoma Borders
title_short Diffusion Reflection Measurements of Antibodies Conjugated to Gold Nanoparticles as a Method to Identify Cutaneous Squamous Cell Carcinoma Borders
title_sort diffusion reflection measurements of antibodies conjugated to gold nanoparticles as a method to identify cutaneous squamous cell carcinoma borders
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7014005/
https://www.ncbi.nlm.nih.gov/pubmed/31963462
http://dx.doi.org/10.3390/ma13020447
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