Cargando…

Evidence of hybridization, mitochondrial introgression and biparental inheritance of the kDNA minicircles in Trypanosoma cruzi I

BACKGROUND: Genetic exchange in Trypanosoma cruzi is controversial not only in relation to its frequency, but also to its mechanism. Parasexual genetic exchange has been proposed based on laboratory hybrids, but population genomics strongly suggests meiosis in T. cruzi. In addition, mitochondrial in...

Descripción completa

Detalles Bibliográficos
Autores principales: Rusman, Fanny, Floridia-Yapur, Noelia, Ragone, Paula G., Diosque, Patricio, Tomasini, Nicolás
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7015434/
https://www.ncbi.nlm.nih.gov/pubmed/32004318
http://dx.doi.org/10.1371/journal.pntd.0007770
_version_ 1783496797589602304
author Rusman, Fanny
Floridia-Yapur, Noelia
Ragone, Paula G.
Diosque, Patricio
Tomasini, Nicolás
author_facet Rusman, Fanny
Floridia-Yapur, Noelia
Ragone, Paula G.
Diosque, Patricio
Tomasini, Nicolás
author_sort Rusman, Fanny
collection PubMed
description BACKGROUND: Genetic exchange in Trypanosoma cruzi is controversial not only in relation to its frequency, but also to its mechanism. Parasexual genetic exchange has been proposed based on laboratory hybrids, but population genomics strongly suggests meiosis in T. cruzi. In addition, mitochondrial introgression has been reported several times in natural isolates although its mechanism is not fully understood yet. Moreover, hybrid T. cruzi DTUs (TcV and TcVI) have inherited at least part of the kinetoplastic DNA (kDNA = mitochondrial DNA) from both parents. METHODOLOGY/PRINCIPAL FINDINGS: In order to address such topics, we sequenced and analyzed fourteen nuclear DNA fragments and three kDNA maxicircle genes in three TcI stocks which are natural clones potentially involved in events of genetic exchange. We also deep-sequenced (a total of 6,146,686 paired-end reads) the minicircle hypervariable region (mHVR) of the kDNA in such three strains. In addition, we analyzed the DNA content by flow cytometry to address cell ploidy. We observed that most polymorphic sites in nuclear loci showed a hybrid pattern in one cloned strain and the other two cloned strains were compatible as parental strains (or nearly related to the true parents). The three clones had almost the same ploidy and the DNA content was similar to the reference strain Sylvio (a nearly diploid strain). Despite maxicircle genes evolve faster than nuclear housekeeping ones, we detected no polymorphisms in the sequence of three maxicircle genes showing mito-nuclear discordance. Lastly, the hybrid stock shared 66% of its mHVR clusters with one putative parent and 47% with the other one; in contrast, the putative parental stocks shared less than 30% of the mHVR clusters between them. CONCLUSIONS/SIGNIFICANCE: The results suggest a reductive division, a natural hybridization, biparental inheritance of the minicircles in the hybrid and maxicircle introgression. The models including such phenomena and explaining the relationships between these three clones are discussed.
format Online
Article
Text
id pubmed-7015434
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-70154342020-02-26 Evidence of hybridization, mitochondrial introgression and biparental inheritance of the kDNA minicircles in Trypanosoma cruzi I Rusman, Fanny Floridia-Yapur, Noelia Ragone, Paula G. Diosque, Patricio Tomasini, Nicolás PLoS Negl Trop Dis Research Article BACKGROUND: Genetic exchange in Trypanosoma cruzi is controversial not only in relation to its frequency, but also to its mechanism. Parasexual genetic exchange has been proposed based on laboratory hybrids, but population genomics strongly suggests meiosis in T. cruzi. In addition, mitochondrial introgression has been reported several times in natural isolates although its mechanism is not fully understood yet. Moreover, hybrid T. cruzi DTUs (TcV and TcVI) have inherited at least part of the kinetoplastic DNA (kDNA = mitochondrial DNA) from both parents. METHODOLOGY/PRINCIPAL FINDINGS: In order to address such topics, we sequenced and analyzed fourteen nuclear DNA fragments and three kDNA maxicircle genes in three TcI stocks which are natural clones potentially involved in events of genetic exchange. We also deep-sequenced (a total of 6,146,686 paired-end reads) the minicircle hypervariable region (mHVR) of the kDNA in such three strains. In addition, we analyzed the DNA content by flow cytometry to address cell ploidy. We observed that most polymorphic sites in nuclear loci showed a hybrid pattern in one cloned strain and the other two cloned strains were compatible as parental strains (or nearly related to the true parents). The three clones had almost the same ploidy and the DNA content was similar to the reference strain Sylvio (a nearly diploid strain). Despite maxicircle genes evolve faster than nuclear housekeeping ones, we detected no polymorphisms in the sequence of three maxicircle genes showing mito-nuclear discordance. Lastly, the hybrid stock shared 66% of its mHVR clusters with one putative parent and 47% with the other one; in contrast, the putative parental stocks shared less than 30% of the mHVR clusters between them. CONCLUSIONS/SIGNIFICANCE: The results suggest a reductive division, a natural hybridization, biparental inheritance of the minicircles in the hybrid and maxicircle introgression. The models including such phenomena and explaining the relationships between these three clones are discussed. Public Library of Science 2020-01-31 /pmc/articles/PMC7015434/ /pubmed/32004318 http://dx.doi.org/10.1371/journal.pntd.0007770 Text en © 2020 Rusman et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Rusman, Fanny
Floridia-Yapur, Noelia
Ragone, Paula G.
Diosque, Patricio
Tomasini, Nicolás
Evidence of hybridization, mitochondrial introgression and biparental inheritance of the kDNA minicircles in Trypanosoma cruzi I
title Evidence of hybridization, mitochondrial introgression and biparental inheritance of the kDNA minicircles in Trypanosoma cruzi I
title_full Evidence of hybridization, mitochondrial introgression and biparental inheritance of the kDNA minicircles in Trypanosoma cruzi I
title_fullStr Evidence of hybridization, mitochondrial introgression and biparental inheritance of the kDNA minicircles in Trypanosoma cruzi I
title_full_unstemmed Evidence of hybridization, mitochondrial introgression and biparental inheritance of the kDNA minicircles in Trypanosoma cruzi I
title_short Evidence of hybridization, mitochondrial introgression and biparental inheritance of the kDNA minicircles in Trypanosoma cruzi I
title_sort evidence of hybridization, mitochondrial introgression and biparental inheritance of the kdna minicircles in trypanosoma cruzi i
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7015434/
https://www.ncbi.nlm.nih.gov/pubmed/32004318
http://dx.doi.org/10.1371/journal.pntd.0007770
work_keys_str_mv AT rusmanfanny evidenceofhybridizationmitochondrialintrogressionandbiparentalinheritanceofthekdnaminicirclesintrypanosomacruzii
AT floridiayapurnoelia evidenceofhybridizationmitochondrialintrogressionandbiparentalinheritanceofthekdnaminicirclesintrypanosomacruzii
AT ragonepaulag evidenceofhybridizationmitochondrialintrogressionandbiparentalinheritanceofthekdnaminicirclesintrypanosomacruzii
AT diosquepatricio evidenceofhybridizationmitochondrialintrogressionandbiparentalinheritanceofthekdnaminicirclesintrypanosomacruzii
AT tomasininicolas evidenceofhybridizationmitochondrialintrogressionandbiparentalinheritanceofthekdnaminicirclesintrypanosomacruzii