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In-cell NMR as a sensitive tool to monitor physiological condition of Escherichia coli

The in-cell NMR technique offers significant insights into the structure and function of heterologous proteins in the physiological intracellular environment at an atomic resolution. Escherichia coli (E. coli) is one of the most widely used host cells for heterologous protein expression in structura...

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Detalles Bibliográficos
Autores principales: Sugiki, Toshihiko, Yamaguchi, Yoshihiro, Fujiwara, Toshimichi, Inouye, Masayori, Ito, Yutaka, Kojima, Chojiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7015911/
https://www.ncbi.nlm.nih.gov/pubmed/32051433
http://dx.doi.org/10.1038/s41598-020-59076-2