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In-cell NMR as a sensitive tool to monitor physiological condition of Escherichia coli
The in-cell NMR technique offers significant insights into the structure and function of heterologous proteins in the physiological intracellular environment at an atomic resolution. Escherichia coli (E. coli) is one of the most widely used host cells for heterologous protein expression in structura...
Autores principales: | Sugiki, Toshihiko, Yamaguchi, Yoshihiro, Fujiwara, Toshimichi, Inouye, Masayori, Ito, Yutaka, Kojima, Chojiro |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7015911/ https://www.ncbi.nlm.nih.gov/pubmed/32051433 http://dx.doi.org/10.1038/s41598-020-59076-2 |
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