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Microbial Signatures and Innate Immune Gene Expression in Lamina Propria Phagocytes of Inflammatory Bowel Disease Patients

BACKGROUND & AIMS: The interaction between intestinal microbiota and the immune system plays a vital role in inflammatory bowel disease (IBD). Although numerous deep-sequencing studies have suggested dysbiosis in IBD, identifying specific bacteria from the stool or mucosa that are responsible fo...

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Autores principales: Dheer, Rishu, Davies, Julie M., Quintero, Maria A., Damas, Oriana M., Deshpande, Amar R., Kerman, David H., Sawyer, William Peter, Pignac-Kobinger, Judith, Ban, Yuguang, Fernandez, Irina, Burgueno, Juan F., Phillips, Matthew C., Abreu, Maria T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7015995/
https://www.ncbi.nlm.nih.gov/pubmed/31740421
http://dx.doi.org/10.1016/j.jcmgh.2019.10.013
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author Dheer, Rishu
Davies, Julie M.
Quintero, Maria A.
Damas, Oriana M.
Deshpande, Amar R.
Kerman, David H.
Sawyer, William Peter
Pignac-Kobinger, Judith
Ban, Yuguang
Fernandez, Irina
Burgueno, Juan F.
Phillips, Matthew C.
Abreu, Maria T.
author_facet Dheer, Rishu
Davies, Julie M.
Quintero, Maria A.
Damas, Oriana M.
Deshpande, Amar R.
Kerman, David H.
Sawyer, William Peter
Pignac-Kobinger, Judith
Ban, Yuguang
Fernandez, Irina
Burgueno, Juan F.
Phillips, Matthew C.
Abreu, Maria T.
author_sort Dheer, Rishu
collection PubMed
description BACKGROUND & AIMS: The interaction between intestinal microbiota and the immune system plays a vital role in inflammatory bowel disease (IBD). Although numerous deep-sequencing studies have suggested dysbiosis in IBD, identifying specific bacteria from the stool or mucosa that are responsible for disease susceptibility or severity has remained a challenge. Lamina propria phagocytes ideally are localized to interact with bacteria that are in close proximity to, or have invaded, the tissue. Thus, we examined the microbial populations associated with the lamina propria phagocytes in 20 Crohn’s disease and 12 ulcerative colitis patients. Specifically, we aimed to address whether the phagocyte-associated microbiota differed from the mucosa-associated microbiota and whether this varied based on IBD type or the state of inflammation. METHODS: 16S ribosomal RNA gene sequencing and innate immune gene expression profiling was done on CD11b+ lamina propria phagocytes isolated from the biopsies obtained from IBD patients. RESULTS: Phagocyte-associated microbiota was enriched in bacterial species belonging to phylum Proteobacteria, whereas species belonging to phylum Bacteroidetes were enriched in the mucosal microbiota of IBD patients. Disease type was the most influential factor in driving differences in the microbiota of both the mucosa and the lamina propria phagocytes, irrespective of inflammation state o`r anatomic location. Crohn’s disease and ulcerative colitis specimens showed similar patterns of increased inflammatory gene expression in phagocytes isolated from inflamed areas compared with those isolated from uninflamed regions. CONCLUSIONS: This pilot study shows the feasibility of using lamina propria phagocytes to characterize the microbiota in IBD patients. The approach used in this study can narrow the spectrum of potentially dysbiotic bacterial populations and clinically relevant gene expression signatures in IBD patients.
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spelling pubmed-70159952020-02-18 Microbial Signatures and Innate Immune Gene Expression in Lamina Propria Phagocytes of Inflammatory Bowel Disease Patients Dheer, Rishu Davies, Julie M. Quintero, Maria A. Damas, Oriana M. Deshpande, Amar R. Kerman, David H. Sawyer, William Peter Pignac-Kobinger, Judith Ban, Yuguang Fernandez, Irina Burgueno, Juan F. Phillips, Matthew C. Abreu, Maria T. Cell Mol Gastroenterol Hepatol Original Research BACKGROUND & AIMS: The interaction between intestinal microbiota and the immune system plays a vital role in inflammatory bowel disease (IBD). Although numerous deep-sequencing studies have suggested dysbiosis in IBD, identifying specific bacteria from the stool or mucosa that are responsible for disease susceptibility or severity has remained a challenge. Lamina propria phagocytes ideally are localized to interact with bacteria that are in close proximity to, or have invaded, the tissue. Thus, we examined the microbial populations associated with the lamina propria phagocytes in 20 Crohn’s disease and 12 ulcerative colitis patients. Specifically, we aimed to address whether the phagocyte-associated microbiota differed from the mucosa-associated microbiota and whether this varied based on IBD type or the state of inflammation. METHODS: 16S ribosomal RNA gene sequencing and innate immune gene expression profiling was done on CD11b+ lamina propria phagocytes isolated from the biopsies obtained from IBD patients. RESULTS: Phagocyte-associated microbiota was enriched in bacterial species belonging to phylum Proteobacteria, whereas species belonging to phylum Bacteroidetes were enriched in the mucosal microbiota of IBD patients. Disease type was the most influential factor in driving differences in the microbiota of both the mucosa and the lamina propria phagocytes, irrespective of inflammation state o`r anatomic location. Crohn’s disease and ulcerative colitis specimens showed similar patterns of increased inflammatory gene expression in phagocytes isolated from inflamed areas compared with those isolated from uninflamed regions. CONCLUSIONS: This pilot study shows the feasibility of using lamina propria phagocytes to characterize the microbiota in IBD patients. The approach used in this study can narrow the spectrum of potentially dysbiotic bacterial populations and clinically relevant gene expression signatures in IBD patients. Elsevier 2019-11-15 /pmc/articles/PMC7015995/ /pubmed/31740421 http://dx.doi.org/10.1016/j.jcmgh.2019.10.013 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Research
Dheer, Rishu
Davies, Julie M.
Quintero, Maria A.
Damas, Oriana M.
Deshpande, Amar R.
Kerman, David H.
Sawyer, William Peter
Pignac-Kobinger, Judith
Ban, Yuguang
Fernandez, Irina
Burgueno, Juan F.
Phillips, Matthew C.
Abreu, Maria T.
Microbial Signatures and Innate Immune Gene Expression in Lamina Propria Phagocytes of Inflammatory Bowel Disease Patients
title Microbial Signatures and Innate Immune Gene Expression in Lamina Propria Phagocytes of Inflammatory Bowel Disease Patients
title_full Microbial Signatures and Innate Immune Gene Expression in Lamina Propria Phagocytes of Inflammatory Bowel Disease Patients
title_fullStr Microbial Signatures and Innate Immune Gene Expression in Lamina Propria Phagocytes of Inflammatory Bowel Disease Patients
title_full_unstemmed Microbial Signatures and Innate Immune Gene Expression in Lamina Propria Phagocytes of Inflammatory Bowel Disease Patients
title_short Microbial Signatures and Innate Immune Gene Expression in Lamina Propria Phagocytes of Inflammatory Bowel Disease Patients
title_sort microbial signatures and innate immune gene expression in lamina propria phagocytes of inflammatory bowel disease patients
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7015995/
https://www.ncbi.nlm.nih.gov/pubmed/31740421
http://dx.doi.org/10.1016/j.jcmgh.2019.10.013
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