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Mechanical Regulation Underlies Effects of Exercise on Serotonin-Induced Signaling in the Prefrontal Cortex Neurons
Mechanical forces are known to be involved in various biological processes. However, it remains unclear whether brain functions are mechanically regulated under physiological conditions. Here, we demonstrate that treadmill running and passive head motion (PHM), both of which produce mechanical impac...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016263/ https://www.ncbi.nlm.nih.gov/pubmed/32062453 http://dx.doi.org/10.1016/j.isci.2020.100874 |
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author | Ryu, Youngjae Maekawa, Takahiro Yoshino, Daisuke Sakitani, Naoyoshi Takashima, Atsushi Inoue, Takenobu Suzurikawa, Jun Toyohara, Jun Tago, Tetsuro Makuuchi, Michiru Fujita, Naoki Sawada, Keisuke Murase, Shuhei Watanave, Masashi Hirai, Hirokazu Sakai, Takamasa Yoshikawa, Yuki Ogata, Toru Shinohara, Masahiro Nagao, Motoshi Sawada, Yasuhiro |
author_facet | Ryu, Youngjae Maekawa, Takahiro Yoshino, Daisuke Sakitani, Naoyoshi Takashima, Atsushi Inoue, Takenobu Suzurikawa, Jun Toyohara, Jun Tago, Tetsuro Makuuchi, Michiru Fujita, Naoki Sawada, Keisuke Murase, Shuhei Watanave, Masashi Hirai, Hirokazu Sakai, Takamasa Yoshikawa, Yuki Ogata, Toru Shinohara, Masahiro Nagao, Motoshi Sawada, Yasuhiro |
author_sort | Ryu, Youngjae |
collection | PubMed |
description | Mechanical forces are known to be involved in various biological processes. However, it remains unclear whether brain functions are mechanically regulated under physiological conditions. Here, we demonstrate that treadmill running and passive head motion (PHM), both of which produce mechanical impact on the head, have similar effects on the hallucinogenic 5-hydroxytryptamine (5-HT) receptor subtype 2A (5-HT(2A)) signaling in the prefrontal cortex (PFC) of rodents. PHM generates interstitial fluid movement that is estimated to exert shear stress of a few pascals on cells in the PFC. Fluid shear stress of a relevant magnitude on cultured neuronal cells induces ligand-independent internalization of 5-HT(2A) receptor, which is observed in mouse PFC neurons after treadmill running or PHM. Furthermore, inhibition of interstitial fluid movement by introducing polyethylene glycol hydrogel eliminates the effect of PHM on 5-HT(2A) receptor signaling in the PFC. Our findings indicate that neuronal cell function can be physiologically regulated by mechanical forces in the brain. |
format | Online Article Text |
id | pubmed-7016263 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-70162632020-02-18 Mechanical Regulation Underlies Effects of Exercise on Serotonin-Induced Signaling in the Prefrontal Cortex Neurons Ryu, Youngjae Maekawa, Takahiro Yoshino, Daisuke Sakitani, Naoyoshi Takashima, Atsushi Inoue, Takenobu Suzurikawa, Jun Toyohara, Jun Tago, Tetsuro Makuuchi, Michiru Fujita, Naoki Sawada, Keisuke Murase, Shuhei Watanave, Masashi Hirai, Hirokazu Sakai, Takamasa Yoshikawa, Yuki Ogata, Toru Shinohara, Masahiro Nagao, Motoshi Sawada, Yasuhiro iScience Article Mechanical forces are known to be involved in various biological processes. However, it remains unclear whether brain functions are mechanically regulated under physiological conditions. Here, we demonstrate that treadmill running and passive head motion (PHM), both of which produce mechanical impact on the head, have similar effects on the hallucinogenic 5-hydroxytryptamine (5-HT) receptor subtype 2A (5-HT(2A)) signaling in the prefrontal cortex (PFC) of rodents. PHM generates interstitial fluid movement that is estimated to exert shear stress of a few pascals on cells in the PFC. Fluid shear stress of a relevant magnitude on cultured neuronal cells induces ligand-independent internalization of 5-HT(2A) receptor, which is observed in mouse PFC neurons after treadmill running or PHM. Furthermore, inhibition of interstitial fluid movement by introducing polyethylene glycol hydrogel eliminates the effect of PHM on 5-HT(2A) receptor signaling in the PFC. Our findings indicate that neuronal cell function can be physiologically regulated by mechanical forces in the brain. Elsevier 2020-01-31 /pmc/articles/PMC7016263/ /pubmed/32062453 http://dx.doi.org/10.1016/j.isci.2020.100874 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ryu, Youngjae Maekawa, Takahiro Yoshino, Daisuke Sakitani, Naoyoshi Takashima, Atsushi Inoue, Takenobu Suzurikawa, Jun Toyohara, Jun Tago, Tetsuro Makuuchi, Michiru Fujita, Naoki Sawada, Keisuke Murase, Shuhei Watanave, Masashi Hirai, Hirokazu Sakai, Takamasa Yoshikawa, Yuki Ogata, Toru Shinohara, Masahiro Nagao, Motoshi Sawada, Yasuhiro Mechanical Regulation Underlies Effects of Exercise on Serotonin-Induced Signaling in the Prefrontal Cortex Neurons |
title | Mechanical Regulation Underlies Effects of Exercise on Serotonin-Induced Signaling in the Prefrontal Cortex Neurons |
title_full | Mechanical Regulation Underlies Effects of Exercise on Serotonin-Induced Signaling in the Prefrontal Cortex Neurons |
title_fullStr | Mechanical Regulation Underlies Effects of Exercise on Serotonin-Induced Signaling in the Prefrontal Cortex Neurons |
title_full_unstemmed | Mechanical Regulation Underlies Effects of Exercise on Serotonin-Induced Signaling in the Prefrontal Cortex Neurons |
title_short | Mechanical Regulation Underlies Effects of Exercise on Serotonin-Induced Signaling in the Prefrontal Cortex Neurons |
title_sort | mechanical regulation underlies effects of exercise on serotonin-induced signaling in the prefrontal cortex neurons |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016263/ https://www.ncbi.nlm.nih.gov/pubmed/32062453 http://dx.doi.org/10.1016/j.isci.2020.100874 |
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