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Missing Information from the Estrogen Receptor Puzzle: Where Are They Localized in Bull Reproductive Tissues and Spermatozoa?

Estrogens are steroid hormones that affect a wide range of physiological functions. The effect of estrogens on male reproductive tissues and sperm cells through specific receptors is essential for sperm development, maturation, and function. Although estrogen receptors (ERs) have been studied in sev...

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Autores principales: Antalikova, Jana, Secova, Petra, Horovska, Lubica, Krejcirova, Romana, Simonik, Ondrej, Jankovicova, Jana, Bartokova, Michaela, Tumova, Lucie, Manaskova-Postlerova, Pavla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016540/
https://www.ncbi.nlm.nih.gov/pubmed/31936899
http://dx.doi.org/10.3390/cells9010183
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author Antalikova, Jana
Secova, Petra
Horovska, Lubica
Krejcirova, Romana
Simonik, Ondrej
Jankovicova, Jana
Bartokova, Michaela
Tumova, Lucie
Manaskova-Postlerova, Pavla
author_facet Antalikova, Jana
Secova, Petra
Horovska, Lubica
Krejcirova, Romana
Simonik, Ondrej
Jankovicova, Jana
Bartokova, Michaela
Tumova, Lucie
Manaskova-Postlerova, Pavla
author_sort Antalikova, Jana
collection PubMed
description Estrogens are steroid hormones that affect a wide range of physiological functions. The effect of estrogens on male reproductive tissues and sperm cells through specific receptors is essential for sperm development, maturation, and function. Although estrogen receptors (ERs) have been studied in several mammalian species, including humans, they have not yet been described in bull spermatozoa and reproductive tissues. In this study, we analyzed the presence of all types of ERs (ESR1, ESR2, and GPER1) in bull testicular and epididymal tissues and epididymal and ejaculated spermatozoa, and we characterize them here for the first time. We observed different localizations of each type of ER in the sperm head by immunofluorescent microscopy. Additionally, using a selected polyclonal antibody, we found that each type of ER in bull sperm extracts had two isoforms with different molecular masses. The detailed detection of ERs is a prerequisite not only for understanding the effect of estrogen on all reproductive events but also for further studying the negative effect of environmental estrogens (endocrine disruptors) on processes that lead to fertilization.
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spelling pubmed-70165402020-03-04 Missing Information from the Estrogen Receptor Puzzle: Where Are They Localized in Bull Reproductive Tissues and Spermatozoa? Antalikova, Jana Secova, Petra Horovska, Lubica Krejcirova, Romana Simonik, Ondrej Jankovicova, Jana Bartokova, Michaela Tumova, Lucie Manaskova-Postlerova, Pavla Cells Article Estrogens are steroid hormones that affect a wide range of physiological functions. The effect of estrogens on male reproductive tissues and sperm cells through specific receptors is essential for sperm development, maturation, and function. Although estrogen receptors (ERs) have been studied in several mammalian species, including humans, they have not yet been described in bull spermatozoa and reproductive tissues. In this study, we analyzed the presence of all types of ERs (ESR1, ESR2, and GPER1) in bull testicular and epididymal tissues and epididymal and ejaculated spermatozoa, and we characterize them here for the first time. We observed different localizations of each type of ER in the sperm head by immunofluorescent microscopy. Additionally, using a selected polyclonal antibody, we found that each type of ER in bull sperm extracts had two isoforms with different molecular masses. The detailed detection of ERs is a prerequisite not only for understanding the effect of estrogen on all reproductive events but also for further studying the negative effect of environmental estrogens (endocrine disruptors) on processes that lead to fertilization. MDPI 2020-01-10 /pmc/articles/PMC7016540/ /pubmed/31936899 http://dx.doi.org/10.3390/cells9010183 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Antalikova, Jana
Secova, Petra
Horovska, Lubica
Krejcirova, Romana
Simonik, Ondrej
Jankovicova, Jana
Bartokova, Michaela
Tumova, Lucie
Manaskova-Postlerova, Pavla
Missing Information from the Estrogen Receptor Puzzle: Where Are They Localized in Bull Reproductive Tissues and Spermatozoa?
title Missing Information from the Estrogen Receptor Puzzle: Where Are They Localized in Bull Reproductive Tissues and Spermatozoa?
title_full Missing Information from the Estrogen Receptor Puzzle: Where Are They Localized in Bull Reproductive Tissues and Spermatozoa?
title_fullStr Missing Information from the Estrogen Receptor Puzzle: Where Are They Localized in Bull Reproductive Tissues and Spermatozoa?
title_full_unstemmed Missing Information from the Estrogen Receptor Puzzle: Where Are They Localized in Bull Reproductive Tissues and Spermatozoa?
title_short Missing Information from the Estrogen Receptor Puzzle: Where Are They Localized in Bull Reproductive Tissues and Spermatozoa?
title_sort missing information from the estrogen receptor puzzle: where are they localized in bull reproductive tissues and spermatozoa?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016540/
https://www.ncbi.nlm.nih.gov/pubmed/31936899
http://dx.doi.org/10.3390/cells9010183
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