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The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans
The velvet regulator VosA plays a pivotal role in asexual sporulation in the model filamentous fungus Aspergillus nidulans. In the present study, we characterize the roles of VosA in sexual spores (ascospores) in A. nidulans. During ascospore maturation, the deletion of vosA causes a rapid decrease...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016683/ https://www.ncbi.nlm.nih.gov/pubmed/31963266 http://dx.doi.org/10.3390/genes11010103 |
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author | Kim, Min-Ju Lee, Mi-Kyung Pham, Huy Quang Gu, Myeong Ju Zhu, Bohan Son, Sung-Hun Hahn, Dongyup Shin, Jae-Ho Yu, Jae-Hyuk Park, Hee-Soo Han, Kap-Hoon |
author_facet | Kim, Min-Ju Lee, Mi-Kyung Pham, Huy Quang Gu, Myeong Ju Zhu, Bohan Son, Sung-Hun Hahn, Dongyup Shin, Jae-Ho Yu, Jae-Hyuk Park, Hee-Soo Han, Kap-Hoon |
author_sort | Kim, Min-Ju |
collection | PubMed |
description | The velvet regulator VosA plays a pivotal role in asexual sporulation in the model filamentous fungus Aspergillus nidulans. In the present study, we characterize the roles of VosA in sexual spores (ascospores) in A. nidulans. During ascospore maturation, the deletion of vosA causes a rapid decrease in spore viability. The absence of vosA also results in a lack of trehalose biogenesis and decreased tolerance of ascospores to thermal and oxidative stresses. RNA-seq-based genome-wide expression analysis demonstrated that the loss of vosA leads to elevated expression of sterigmatocystin (ST) biosynthetic genes and a slight increase in ST production in ascospores. Moreover, the deletion of vosA causes upregulation of additional gene clusters associated with the biosynthesis of other secondary metabolites, including asperthecin, microperfuranone, and monodictyphenone. On the other hand, the lack of vosA results in the downregulation of various genes involved in primary metabolism. In addition, vosA deletion alters mRNA levels of genes associated with the cell wall integrity and trehalose biosynthesis. Overall, these results demonstrate that the velvet regulator VosA plays a key role in the maturation and the cellular and metabolic integrity of sexual spores in A. nidulans. |
format | Online Article Text |
id | pubmed-7016683 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70166832020-02-28 The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans Kim, Min-Ju Lee, Mi-Kyung Pham, Huy Quang Gu, Myeong Ju Zhu, Bohan Son, Sung-Hun Hahn, Dongyup Shin, Jae-Ho Yu, Jae-Hyuk Park, Hee-Soo Han, Kap-Hoon Genes (Basel) Article The velvet regulator VosA plays a pivotal role in asexual sporulation in the model filamentous fungus Aspergillus nidulans. In the present study, we characterize the roles of VosA in sexual spores (ascospores) in A. nidulans. During ascospore maturation, the deletion of vosA causes a rapid decrease in spore viability. The absence of vosA also results in a lack of trehalose biogenesis and decreased tolerance of ascospores to thermal and oxidative stresses. RNA-seq-based genome-wide expression analysis demonstrated that the loss of vosA leads to elevated expression of sterigmatocystin (ST) biosynthetic genes and a slight increase in ST production in ascospores. Moreover, the deletion of vosA causes upregulation of additional gene clusters associated with the biosynthesis of other secondary metabolites, including asperthecin, microperfuranone, and monodictyphenone. On the other hand, the lack of vosA results in the downregulation of various genes involved in primary metabolism. In addition, vosA deletion alters mRNA levels of genes associated with the cell wall integrity and trehalose biosynthesis. Overall, these results demonstrate that the velvet regulator VosA plays a key role in the maturation and the cellular and metabolic integrity of sexual spores in A. nidulans. MDPI 2020-01-16 /pmc/articles/PMC7016683/ /pubmed/31963266 http://dx.doi.org/10.3390/genes11010103 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Min-Ju Lee, Mi-Kyung Pham, Huy Quang Gu, Myeong Ju Zhu, Bohan Son, Sung-Hun Hahn, Dongyup Shin, Jae-Ho Yu, Jae-Hyuk Park, Hee-Soo Han, Kap-Hoon The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans |
title | The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans |
title_full | The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans |
title_fullStr | The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans |
title_full_unstemmed | The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans |
title_short | The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans |
title_sort | velvet regulator vosa governs survival and secondary metabolism of sexual spores in aspergillus nidulans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016683/ https://www.ncbi.nlm.nih.gov/pubmed/31963266 http://dx.doi.org/10.3390/genes11010103 |
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