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The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans

The velvet regulator VosA plays a pivotal role in asexual sporulation in the model filamentous fungus Aspergillus nidulans. In the present study, we characterize the roles of VosA in sexual spores (ascospores) in A. nidulans. During ascospore maturation, the deletion of vosA causes a rapid decrease...

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Autores principales: Kim, Min-Ju, Lee, Mi-Kyung, Pham, Huy Quang, Gu, Myeong Ju, Zhu, Bohan, Son, Sung-Hun, Hahn, Dongyup, Shin, Jae-Ho, Yu, Jae-Hyuk, Park, Hee-Soo, Han, Kap-Hoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016683/
https://www.ncbi.nlm.nih.gov/pubmed/31963266
http://dx.doi.org/10.3390/genes11010103
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author Kim, Min-Ju
Lee, Mi-Kyung
Pham, Huy Quang
Gu, Myeong Ju
Zhu, Bohan
Son, Sung-Hun
Hahn, Dongyup
Shin, Jae-Ho
Yu, Jae-Hyuk
Park, Hee-Soo
Han, Kap-Hoon
author_facet Kim, Min-Ju
Lee, Mi-Kyung
Pham, Huy Quang
Gu, Myeong Ju
Zhu, Bohan
Son, Sung-Hun
Hahn, Dongyup
Shin, Jae-Ho
Yu, Jae-Hyuk
Park, Hee-Soo
Han, Kap-Hoon
author_sort Kim, Min-Ju
collection PubMed
description The velvet regulator VosA plays a pivotal role in asexual sporulation in the model filamentous fungus Aspergillus nidulans. In the present study, we characterize the roles of VosA in sexual spores (ascospores) in A. nidulans. During ascospore maturation, the deletion of vosA causes a rapid decrease in spore viability. The absence of vosA also results in a lack of trehalose biogenesis and decreased tolerance of ascospores to thermal and oxidative stresses. RNA-seq-based genome-wide expression analysis demonstrated that the loss of vosA leads to elevated expression of sterigmatocystin (ST) biosynthetic genes and a slight increase in ST production in ascospores. Moreover, the deletion of vosA causes upregulation of additional gene clusters associated with the biosynthesis of other secondary metabolites, including asperthecin, microperfuranone, and monodictyphenone. On the other hand, the lack of vosA results in the downregulation of various genes involved in primary metabolism. In addition, vosA deletion alters mRNA levels of genes associated with the cell wall integrity and trehalose biosynthesis. Overall, these results demonstrate that the velvet regulator VosA plays a key role in the maturation and the cellular and metabolic integrity of sexual spores in A. nidulans.
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spelling pubmed-70166832020-02-28 The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans Kim, Min-Ju Lee, Mi-Kyung Pham, Huy Quang Gu, Myeong Ju Zhu, Bohan Son, Sung-Hun Hahn, Dongyup Shin, Jae-Ho Yu, Jae-Hyuk Park, Hee-Soo Han, Kap-Hoon Genes (Basel) Article The velvet regulator VosA plays a pivotal role in asexual sporulation in the model filamentous fungus Aspergillus nidulans. In the present study, we characterize the roles of VosA in sexual spores (ascospores) in A. nidulans. During ascospore maturation, the deletion of vosA causes a rapid decrease in spore viability. The absence of vosA also results in a lack of trehalose biogenesis and decreased tolerance of ascospores to thermal and oxidative stresses. RNA-seq-based genome-wide expression analysis demonstrated that the loss of vosA leads to elevated expression of sterigmatocystin (ST) biosynthetic genes and a slight increase in ST production in ascospores. Moreover, the deletion of vosA causes upregulation of additional gene clusters associated with the biosynthesis of other secondary metabolites, including asperthecin, microperfuranone, and monodictyphenone. On the other hand, the lack of vosA results in the downregulation of various genes involved in primary metabolism. In addition, vosA deletion alters mRNA levels of genes associated with the cell wall integrity and trehalose biosynthesis. Overall, these results demonstrate that the velvet regulator VosA plays a key role in the maturation and the cellular and metabolic integrity of sexual spores in A. nidulans. MDPI 2020-01-16 /pmc/articles/PMC7016683/ /pubmed/31963266 http://dx.doi.org/10.3390/genes11010103 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Min-Ju
Lee, Mi-Kyung
Pham, Huy Quang
Gu, Myeong Ju
Zhu, Bohan
Son, Sung-Hun
Hahn, Dongyup
Shin, Jae-Ho
Yu, Jae-Hyuk
Park, Hee-Soo
Han, Kap-Hoon
The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans
title The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans
title_full The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans
title_fullStr The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans
title_full_unstemmed The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans
title_short The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans
title_sort velvet regulator vosa governs survival and secondary metabolism of sexual spores in aspergillus nidulans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016683/
https://www.ncbi.nlm.nih.gov/pubmed/31963266
http://dx.doi.org/10.3390/genes11010103
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