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Schizosaccharomyces pombe Assays to Study Mitotic Recombination Outcomes
The fission yeast—Schizosaccharomyces pombe—has emerged as a powerful tractable system for studying DNA damage repair. Over the last few decades, several powerful in vivo genetic assays have been developed to study outcomes of mitotic recombination, the major repair mechanism of DNA double strand br...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016768/ https://www.ncbi.nlm.nih.gov/pubmed/31936815 http://dx.doi.org/10.3390/genes11010079 |
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author | Hylton, Hannah M. Lucas, Bailey E. Petreaca, Ruben C. |
author_facet | Hylton, Hannah M. Lucas, Bailey E. Petreaca, Ruben C. |
author_sort | Hylton, Hannah M. |
collection | PubMed |
description | The fission yeast—Schizosaccharomyces pombe—has emerged as a powerful tractable system for studying DNA damage repair. Over the last few decades, several powerful in vivo genetic assays have been developed to study outcomes of mitotic recombination, the major repair mechanism of DNA double strand breaks and stalled or collapsed DNA replication forks. These assays have significantly increased our understanding of the molecular mechanisms underlying the DNA damage response pathways. Here, we review the assays that have been developed in fission yeast to study mitotic recombination. |
format | Online Article Text |
id | pubmed-7016768 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70167682020-02-28 Schizosaccharomyces pombe Assays to Study Mitotic Recombination Outcomes Hylton, Hannah M. Lucas, Bailey E. Petreaca, Ruben C. Genes (Basel) Review The fission yeast—Schizosaccharomyces pombe—has emerged as a powerful tractable system for studying DNA damage repair. Over the last few decades, several powerful in vivo genetic assays have been developed to study outcomes of mitotic recombination, the major repair mechanism of DNA double strand breaks and stalled or collapsed DNA replication forks. These assays have significantly increased our understanding of the molecular mechanisms underlying the DNA damage response pathways. Here, we review the assays that have been developed in fission yeast to study mitotic recombination. MDPI 2020-01-10 /pmc/articles/PMC7016768/ /pubmed/31936815 http://dx.doi.org/10.3390/genes11010079 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Hylton, Hannah M. Lucas, Bailey E. Petreaca, Ruben C. Schizosaccharomyces pombe Assays to Study Mitotic Recombination Outcomes |
title | Schizosaccharomyces pombe Assays to Study Mitotic Recombination Outcomes |
title_full | Schizosaccharomyces pombe Assays to Study Mitotic Recombination Outcomes |
title_fullStr | Schizosaccharomyces pombe Assays to Study Mitotic Recombination Outcomes |
title_full_unstemmed | Schizosaccharomyces pombe Assays to Study Mitotic Recombination Outcomes |
title_short | Schizosaccharomyces pombe Assays to Study Mitotic Recombination Outcomes |
title_sort | schizosaccharomyces pombe assays to study mitotic recombination outcomes |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016768/ https://www.ncbi.nlm.nih.gov/pubmed/31936815 http://dx.doi.org/10.3390/genes11010079 |
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