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Small RNA Profiling of piRNAs in Colorectal Cancer Identifies Consistent Overexpression of piR-24000 That Correlates Clinically with an Aggressive Disease Phenotype

Piwi-interacting RNAs (piRNAs) represent a novel class of small non-coding RNAs (ncRNAs) that have been shown to have a deregulated expression in several cancers, although their clinical significance in colorectal cancer (CRC) remains unclear. With an aim of delineating the piRNA distribution in CRC...

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Autores principales: Iyer, Deepak Narayanan, Wan, Timothy Ming-Hun, Man, Johnny Hon-Wai, Sin, Ryan Wai-Yan, Li, Xue, Lo, Oswens Siu-Hung, Foo, Dominic Chi-Chung, Pang, Roberta Wen-Chi, Law, Wai-Lun, Ng, Lui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016796/
https://www.ncbi.nlm.nih.gov/pubmed/31940941
http://dx.doi.org/10.3390/cancers12010188
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author Iyer, Deepak Narayanan
Wan, Timothy Ming-Hun
Man, Johnny Hon-Wai
Sin, Ryan Wai-Yan
Li, Xue
Lo, Oswens Siu-Hung
Foo, Dominic Chi-Chung
Pang, Roberta Wen-Chi
Law, Wai-Lun
Ng, Lui
author_facet Iyer, Deepak Narayanan
Wan, Timothy Ming-Hun
Man, Johnny Hon-Wai
Sin, Ryan Wai-Yan
Li, Xue
Lo, Oswens Siu-Hung
Foo, Dominic Chi-Chung
Pang, Roberta Wen-Chi
Law, Wai-Lun
Ng, Lui
author_sort Iyer, Deepak Narayanan
collection PubMed
description Piwi-interacting RNAs (piRNAs) represent a novel class of small non-coding RNAs (ncRNAs) that have been shown to have a deregulated expression in several cancers, although their clinical significance in colorectal cancer (CRC) remains unclear. With an aim of delineating the piRNA distribution in CRC, we conducted a systematic discovery and validation of piRNAs within two clinical cohorts. In the discovery phase, we profiled tumor and adjacent normal tissues from 18 CRC patients by deep sequencing and identified a global piRNA downregulation in CRC. Moreover, we identified piR-24000 as an unexplored piRNA that was significantly overexpressed in CRC. Using qPCR, we validated the overexpression of piR-24000 in 87 CRC patients. Additionally, we identified a significant association between a high expression of piR-24000 and an aggressive CRC phenotype including poor differentiation, presence of distant metastases, and a higher stage. Lastly, ROC analysis demonstrated a strong diagnostic power of piR-24000 in discriminating CRC patients from normal subjects. Taken together, this study provides one of the earliest large-scale reports of the global distribution of piRNAs in CRC. In addition, piR-24000 was identified as a likely oncogene in CRC that can serve as a biomarker or a therapeutic target.
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spelling pubmed-70167962020-02-28 Small RNA Profiling of piRNAs in Colorectal Cancer Identifies Consistent Overexpression of piR-24000 That Correlates Clinically with an Aggressive Disease Phenotype Iyer, Deepak Narayanan Wan, Timothy Ming-Hun Man, Johnny Hon-Wai Sin, Ryan Wai-Yan Li, Xue Lo, Oswens Siu-Hung Foo, Dominic Chi-Chung Pang, Roberta Wen-Chi Law, Wai-Lun Ng, Lui Cancers (Basel) Article Piwi-interacting RNAs (piRNAs) represent a novel class of small non-coding RNAs (ncRNAs) that have been shown to have a deregulated expression in several cancers, although their clinical significance in colorectal cancer (CRC) remains unclear. With an aim of delineating the piRNA distribution in CRC, we conducted a systematic discovery and validation of piRNAs within two clinical cohorts. In the discovery phase, we profiled tumor and adjacent normal tissues from 18 CRC patients by deep sequencing and identified a global piRNA downregulation in CRC. Moreover, we identified piR-24000 as an unexplored piRNA that was significantly overexpressed in CRC. Using qPCR, we validated the overexpression of piR-24000 in 87 CRC patients. Additionally, we identified a significant association between a high expression of piR-24000 and an aggressive CRC phenotype including poor differentiation, presence of distant metastases, and a higher stage. Lastly, ROC analysis demonstrated a strong diagnostic power of piR-24000 in discriminating CRC patients from normal subjects. Taken together, this study provides one of the earliest large-scale reports of the global distribution of piRNAs in CRC. In addition, piR-24000 was identified as a likely oncogene in CRC that can serve as a biomarker or a therapeutic target. MDPI 2020-01-12 /pmc/articles/PMC7016796/ /pubmed/31940941 http://dx.doi.org/10.3390/cancers12010188 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Iyer, Deepak Narayanan
Wan, Timothy Ming-Hun
Man, Johnny Hon-Wai
Sin, Ryan Wai-Yan
Li, Xue
Lo, Oswens Siu-Hung
Foo, Dominic Chi-Chung
Pang, Roberta Wen-Chi
Law, Wai-Lun
Ng, Lui
Small RNA Profiling of piRNAs in Colorectal Cancer Identifies Consistent Overexpression of piR-24000 That Correlates Clinically with an Aggressive Disease Phenotype
title Small RNA Profiling of piRNAs in Colorectal Cancer Identifies Consistent Overexpression of piR-24000 That Correlates Clinically with an Aggressive Disease Phenotype
title_full Small RNA Profiling of piRNAs in Colorectal Cancer Identifies Consistent Overexpression of piR-24000 That Correlates Clinically with an Aggressive Disease Phenotype
title_fullStr Small RNA Profiling of piRNAs in Colorectal Cancer Identifies Consistent Overexpression of piR-24000 That Correlates Clinically with an Aggressive Disease Phenotype
title_full_unstemmed Small RNA Profiling of piRNAs in Colorectal Cancer Identifies Consistent Overexpression of piR-24000 That Correlates Clinically with an Aggressive Disease Phenotype
title_short Small RNA Profiling of piRNAs in Colorectal Cancer Identifies Consistent Overexpression of piR-24000 That Correlates Clinically with an Aggressive Disease Phenotype
title_sort small rna profiling of pirnas in colorectal cancer identifies consistent overexpression of pir-24000 that correlates clinically with an aggressive disease phenotype
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016796/
https://www.ncbi.nlm.nih.gov/pubmed/31940941
http://dx.doi.org/10.3390/cancers12010188
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