Cargando…

Hematopoietic Cells Derived from Cancer Stem Cells Generated from Mouse Induced Pluripotent Stem Cells

Cancer stem cells (CSCs) represent the subpopulation of cancer cells with the ability to differentiate into other cell phenotypes and initiated tumorigenesis. Previously, we reported generating CSCs from mouse induced pluripotent stem cells (miPSCs). Here, we investigated the ability of the CSCs to...

Descripción completa

Detalles Bibliográficos
Autores principales: Hassan, Ghmkin, Afify, Said M., Nair, Neha, Kumon, Kazuki, Osman, Amira, Du, Juan, Mansour, Hager, Abu Quora, Hagar A, Nawara, Hend M, Satoh, Ayano, Zahra, Maram H., Okada, Nobuhiro, Seno, Akimasa, Seno, Masaharu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016803/
https://www.ncbi.nlm.nih.gov/pubmed/31905766
http://dx.doi.org/10.3390/cancers12010082
_version_ 1783497059349823488
author Hassan, Ghmkin
Afify, Said M.
Nair, Neha
Kumon, Kazuki
Osman, Amira
Du, Juan
Mansour, Hager
Abu Quora, Hagar A
Nawara, Hend M
Satoh, Ayano
Zahra, Maram H.
Okada, Nobuhiro
Seno, Akimasa
Seno, Masaharu
author_facet Hassan, Ghmkin
Afify, Said M.
Nair, Neha
Kumon, Kazuki
Osman, Amira
Du, Juan
Mansour, Hager
Abu Quora, Hagar A
Nawara, Hend M
Satoh, Ayano
Zahra, Maram H.
Okada, Nobuhiro
Seno, Akimasa
Seno, Masaharu
author_sort Hassan, Ghmkin
collection PubMed
description Cancer stem cells (CSCs) represent the subpopulation of cancer cells with the ability to differentiate into other cell phenotypes and initiated tumorigenesis. Previously, we reported generating CSCs from mouse induced pluripotent stem cells (miPSCs). Here, we investigated the ability of the CSCs to differentiate into hematopoietic cells. First, the primary cells were isolated from malignant tumors that were formed by the CSCs. Non-adherent cells (NACs) that arose from adherent cells were collected and their viability, as well as the morphology and expression of hematopoietic cell markers, were analyzed. Moreover, NACs were injected into the tail vein of busulfan conditioned Balb/c nude mice. Finally, CSCs were induced to differentiate to macrophages while using IL3 and SCF. The round nucleated NACs were found to be viable, positive for hematopoietic lineage markers and CD34, and expressed hematopoietic markers, just like homing to the bone marrow. When NACs were injected into mice, Wright–Giemsa staining showed that the number of white blood cells got higher than those in the control mice after four weeks. CSCs also showed the ability to differentiate toward macrophages. CSCs were demonstrated to have the potential to provide progenies with hematopoietic markers, morphology, and homing ability to the bone marrow, which could give new insight into the tumor microenvironment according to the plasticity of CSCs.
format Online
Article
Text
id pubmed-7016803
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70168032020-02-28 Hematopoietic Cells Derived from Cancer Stem Cells Generated from Mouse Induced Pluripotent Stem Cells Hassan, Ghmkin Afify, Said M. Nair, Neha Kumon, Kazuki Osman, Amira Du, Juan Mansour, Hager Abu Quora, Hagar A Nawara, Hend M Satoh, Ayano Zahra, Maram H. Okada, Nobuhiro Seno, Akimasa Seno, Masaharu Cancers (Basel) Article Cancer stem cells (CSCs) represent the subpopulation of cancer cells with the ability to differentiate into other cell phenotypes and initiated tumorigenesis. Previously, we reported generating CSCs from mouse induced pluripotent stem cells (miPSCs). Here, we investigated the ability of the CSCs to differentiate into hematopoietic cells. First, the primary cells were isolated from malignant tumors that were formed by the CSCs. Non-adherent cells (NACs) that arose from adherent cells were collected and their viability, as well as the morphology and expression of hematopoietic cell markers, were analyzed. Moreover, NACs were injected into the tail vein of busulfan conditioned Balb/c nude mice. Finally, CSCs were induced to differentiate to macrophages while using IL3 and SCF. The round nucleated NACs were found to be viable, positive for hematopoietic lineage markers and CD34, and expressed hematopoietic markers, just like homing to the bone marrow. When NACs were injected into mice, Wright–Giemsa staining showed that the number of white blood cells got higher than those in the control mice after four weeks. CSCs also showed the ability to differentiate toward macrophages. CSCs were demonstrated to have the potential to provide progenies with hematopoietic markers, morphology, and homing ability to the bone marrow, which could give new insight into the tumor microenvironment according to the plasticity of CSCs. MDPI 2019-12-29 /pmc/articles/PMC7016803/ /pubmed/31905766 http://dx.doi.org/10.3390/cancers12010082 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hassan, Ghmkin
Afify, Said M.
Nair, Neha
Kumon, Kazuki
Osman, Amira
Du, Juan
Mansour, Hager
Abu Quora, Hagar A
Nawara, Hend M
Satoh, Ayano
Zahra, Maram H.
Okada, Nobuhiro
Seno, Akimasa
Seno, Masaharu
Hematopoietic Cells Derived from Cancer Stem Cells Generated from Mouse Induced Pluripotent Stem Cells
title Hematopoietic Cells Derived from Cancer Stem Cells Generated from Mouse Induced Pluripotent Stem Cells
title_full Hematopoietic Cells Derived from Cancer Stem Cells Generated from Mouse Induced Pluripotent Stem Cells
title_fullStr Hematopoietic Cells Derived from Cancer Stem Cells Generated from Mouse Induced Pluripotent Stem Cells
title_full_unstemmed Hematopoietic Cells Derived from Cancer Stem Cells Generated from Mouse Induced Pluripotent Stem Cells
title_short Hematopoietic Cells Derived from Cancer Stem Cells Generated from Mouse Induced Pluripotent Stem Cells
title_sort hematopoietic cells derived from cancer stem cells generated from mouse induced pluripotent stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016803/
https://www.ncbi.nlm.nih.gov/pubmed/31905766
http://dx.doi.org/10.3390/cancers12010082
work_keys_str_mv AT hassanghmkin hematopoieticcellsderivedfromcancerstemcellsgeneratedfrommouseinducedpluripotentstemcells
AT afifysaidm hematopoieticcellsderivedfromcancerstemcellsgeneratedfrommouseinducedpluripotentstemcells
AT nairneha hematopoieticcellsderivedfromcancerstemcellsgeneratedfrommouseinducedpluripotentstemcells
AT kumonkazuki hematopoieticcellsderivedfromcancerstemcellsgeneratedfrommouseinducedpluripotentstemcells
AT osmanamira hematopoieticcellsderivedfromcancerstemcellsgeneratedfrommouseinducedpluripotentstemcells
AT dujuan hematopoieticcellsderivedfromcancerstemcellsgeneratedfrommouseinducedpluripotentstemcells
AT mansourhager hematopoieticcellsderivedfromcancerstemcellsgeneratedfrommouseinducedpluripotentstemcells
AT abuquorahagara hematopoieticcellsderivedfromcancerstemcellsgeneratedfrommouseinducedpluripotentstemcells
AT nawarahendm hematopoieticcellsderivedfromcancerstemcellsgeneratedfrommouseinducedpluripotentstemcells
AT satohayano hematopoieticcellsderivedfromcancerstemcellsgeneratedfrommouseinducedpluripotentstemcells
AT zahramaramh hematopoieticcellsderivedfromcancerstemcellsgeneratedfrommouseinducedpluripotentstemcells
AT okadanobuhiro hematopoieticcellsderivedfromcancerstemcellsgeneratedfrommouseinducedpluripotentstemcells
AT senoakimasa hematopoieticcellsderivedfromcancerstemcellsgeneratedfrommouseinducedpluripotentstemcells
AT senomasaharu hematopoieticcellsderivedfromcancerstemcellsgeneratedfrommouseinducedpluripotentstemcells