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Hematopoietic Cells Derived from Cancer Stem Cells Generated from Mouse Induced Pluripotent Stem Cells
Cancer stem cells (CSCs) represent the subpopulation of cancer cells with the ability to differentiate into other cell phenotypes and initiated tumorigenesis. Previously, we reported generating CSCs from mouse induced pluripotent stem cells (miPSCs). Here, we investigated the ability of the CSCs to...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016803/ https://www.ncbi.nlm.nih.gov/pubmed/31905766 http://dx.doi.org/10.3390/cancers12010082 |
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author | Hassan, Ghmkin Afify, Said M. Nair, Neha Kumon, Kazuki Osman, Amira Du, Juan Mansour, Hager Abu Quora, Hagar A Nawara, Hend M Satoh, Ayano Zahra, Maram H. Okada, Nobuhiro Seno, Akimasa Seno, Masaharu |
author_facet | Hassan, Ghmkin Afify, Said M. Nair, Neha Kumon, Kazuki Osman, Amira Du, Juan Mansour, Hager Abu Quora, Hagar A Nawara, Hend M Satoh, Ayano Zahra, Maram H. Okada, Nobuhiro Seno, Akimasa Seno, Masaharu |
author_sort | Hassan, Ghmkin |
collection | PubMed |
description | Cancer stem cells (CSCs) represent the subpopulation of cancer cells with the ability to differentiate into other cell phenotypes and initiated tumorigenesis. Previously, we reported generating CSCs from mouse induced pluripotent stem cells (miPSCs). Here, we investigated the ability of the CSCs to differentiate into hematopoietic cells. First, the primary cells were isolated from malignant tumors that were formed by the CSCs. Non-adherent cells (NACs) that arose from adherent cells were collected and their viability, as well as the morphology and expression of hematopoietic cell markers, were analyzed. Moreover, NACs were injected into the tail vein of busulfan conditioned Balb/c nude mice. Finally, CSCs were induced to differentiate to macrophages while using IL3 and SCF. The round nucleated NACs were found to be viable, positive for hematopoietic lineage markers and CD34, and expressed hematopoietic markers, just like homing to the bone marrow. When NACs were injected into mice, Wright–Giemsa staining showed that the number of white blood cells got higher than those in the control mice after four weeks. CSCs also showed the ability to differentiate toward macrophages. CSCs were demonstrated to have the potential to provide progenies with hematopoietic markers, morphology, and homing ability to the bone marrow, which could give new insight into the tumor microenvironment according to the plasticity of CSCs. |
format | Online Article Text |
id | pubmed-7016803 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70168032020-02-28 Hematopoietic Cells Derived from Cancer Stem Cells Generated from Mouse Induced Pluripotent Stem Cells Hassan, Ghmkin Afify, Said M. Nair, Neha Kumon, Kazuki Osman, Amira Du, Juan Mansour, Hager Abu Quora, Hagar A Nawara, Hend M Satoh, Ayano Zahra, Maram H. Okada, Nobuhiro Seno, Akimasa Seno, Masaharu Cancers (Basel) Article Cancer stem cells (CSCs) represent the subpopulation of cancer cells with the ability to differentiate into other cell phenotypes and initiated tumorigenesis. Previously, we reported generating CSCs from mouse induced pluripotent stem cells (miPSCs). Here, we investigated the ability of the CSCs to differentiate into hematopoietic cells. First, the primary cells were isolated from malignant tumors that were formed by the CSCs. Non-adherent cells (NACs) that arose from adherent cells were collected and their viability, as well as the morphology and expression of hematopoietic cell markers, were analyzed. Moreover, NACs were injected into the tail vein of busulfan conditioned Balb/c nude mice. Finally, CSCs were induced to differentiate to macrophages while using IL3 and SCF. The round nucleated NACs were found to be viable, positive for hematopoietic lineage markers and CD34, and expressed hematopoietic markers, just like homing to the bone marrow. When NACs were injected into mice, Wright–Giemsa staining showed that the number of white blood cells got higher than those in the control mice after four weeks. CSCs also showed the ability to differentiate toward macrophages. CSCs were demonstrated to have the potential to provide progenies with hematopoietic markers, morphology, and homing ability to the bone marrow, which could give new insight into the tumor microenvironment according to the plasticity of CSCs. MDPI 2019-12-29 /pmc/articles/PMC7016803/ /pubmed/31905766 http://dx.doi.org/10.3390/cancers12010082 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hassan, Ghmkin Afify, Said M. Nair, Neha Kumon, Kazuki Osman, Amira Du, Juan Mansour, Hager Abu Quora, Hagar A Nawara, Hend M Satoh, Ayano Zahra, Maram H. Okada, Nobuhiro Seno, Akimasa Seno, Masaharu Hematopoietic Cells Derived from Cancer Stem Cells Generated from Mouse Induced Pluripotent Stem Cells |
title | Hematopoietic Cells Derived from Cancer Stem Cells Generated from Mouse Induced Pluripotent Stem Cells |
title_full | Hematopoietic Cells Derived from Cancer Stem Cells Generated from Mouse Induced Pluripotent Stem Cells |
title_fullStr | Hematopoietic Cells Derived from Cancer Stem Cells Generated from Mouse Induced Pluripotent Stem Cells |
title_full_unstemmed | Hematopoietic Cells Derived from Cancer Stem Cells Generated from Mouse Induced Pluripotent Stem Cells |
title_short | Hematopoietic Cells Derived from Cancer Stem Cells Generated from Mouse Induced Pluripotent Stem Cells |
title_sort | hematopoietic cells derived from cancer stem cells generated from mouse induced pluripotent stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016803/ https://www.ncbi.nlm.nih.gov/pubmed/31905766 http://dx.doi.org/10.3390/cancers12010082 |
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