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Rapid and Label-Free Isolation of Tumour Cells from the Urine of Patients with Localised Prostate Cancer Using Inertial Microfluidics

During the last decade, isolation of circulating tumour cells via blood liquid biopsy of prostate cancer (PCa) has attracted significant attention as an alternative, or substitute, to conventional diagnostic tests. However, it was previously determined that localised forms of PCa shed a small number...

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Autores principales: Rzhevskiy, Alexey S., Razavi Bazaz, Sajad, Ding, Lin, Kapitannikova, Alina, Sayyadi, Nima, Campbell, Douglas, Walsh, Bradley, Gillatt, David, Ebrahimi Warkiani, Majid, Zvyagin, Andrei V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016827/
https://www.ncbi.nlm.nih.gov/pubmed/31905736
http://dx.doi.org/10.3390/cancers12010081
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author Rzhevskiy, Alexey S.
Razavi Bazaz, Sajad
Ding, Lin
Kapitannikova, Alina
Sayyadi, Nima
Campbell, Douglas
Walsh, Bradley
Gillatt, David
Ebrahimi Warkiani, Majid
Zvyagin, Andrei V.
author_facet Rzhevskiy, Alexey S.
Razavi Bazaz, Sajad
Ding, Lin
Kapitannikova, Alina
Sayyadi, Nima
Campbell, Douglas
Walsh, Bradley
Gillatt, David
Ebrahimi Warkiani, Majid
Zvyagin, Andrei V.
author_sort Rzhevskiy, Alexey S.
collection PubMed
description During the last decade, isolation of circulating tumour cells via blood liquid biopsy of prostate cancer (PCa) has attracted significant attention as an alternative, or substitute, to conventional diagnostic tests. However, it was previously determined that localised forms of PCa shed a small number of cancer cells into the bloodstream, and a large volume of blood is required just for a single test, which is impractical. To address this issue, urine has been used as an alternative to blood for liquid biopsy as a truly non-invasive, patient-friendly test. To this end, we developed a spiral microfluidic chip capable of isolating PCa cells from the urine of PCa patients. Potential clinical utility of the chip was demonstrated using anti-Glypican-1 (GPC-1) antibody as a model of the primary antibody in immunofluorescent assay for identification and detection of the collected tumour cells. The microchannel device was first evaluated using DU-145 cells in a diluted Dulbecco’s phosphate-buffered saline sample, where it demonstrated >85 (±6) % efficiency. The microchannel proved to be functional in at least 79% of cases for capturing GPC1+ putative tumour cells from the urine of patients with localised PCa. More importantly, a correlation was found between the amount of the captured GPC1+ cells and crucial diagnostic and prognostic parameter of localised PCa—Gleason score. Thus, the technique demonstrated promise for further assessment of its diagnostic value in PCa detection, diagnosis, and prognosis.
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spelling pubmed-70168272020-02-28 Rapid and Label-Free Isolation of Tumour Cells from the Urine of Patients with Localised Prostate Cancer Using Inertial Microfluidics Rzhevskiy, Alexey S. Razavi Bazaz, Sajad Ding, Lin Kapitannikova, Alina Sayyadi, Nima Campbell, Douglas Walsh, Bradley Gillatt, David Ebrahimi Warkiani, Majid Zvyagin, Andrei V. Cancers (Basel) Article During the last decade, isolation of circulating tumour cells via blood liquid biopsy of prostate cancer (PCa) has attracted significant attention as an alternative, or substitute, to conventional diagnostic tests. However, it was previously determined that localised forms of PCa shed a small number of cancer cells into the bloodstream, and a large volume of blood is required just for a single test, which is impractical. To address this issue, urine has been used as an alternative to blood for liquid biopsy as a truly non-invasive, patient-friendly test. To this end, we developed a spiral microfluidic chip capable of isolating PCa cells from the urine of PCa patients. Potential clinical utility of the chip was demonstrated using anti-Glypican-1 (GPC-1) antibody as a model of the primary antibody in immunofluorescent assay for identification and detection of the collected tumour cells. The microchannel device was first evaluated using DU-145 cells in a diluted Dulbecco’s phosphate-buffered saline sample, where it demonstrated >85 (±6) % efficiency. The microchannel proved to be functional in at least 79% of cases for capturing GPC1+ putative tumour cells from the urine of patients with localised PCa. More importantly, a correlation was found between the amount of the captured GPC1+ cells and crucial diagnostic and prognostic parameter of localised PCa—Gleason score. Thus, the technique demonstrated promise for further assessment of its diagnostic value in PCa detection, diagnosis, and prognosis. MDPI 2019-12-29 /pmc/articles/PMC7016827/ /pubmed/31905736 http://dx.doi.org/10.3390/cancers12010081 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rzhevskiy, Alexey S.
Razavi Bazaz, Sajad
Ding, Lin
Kapitannikova, Alina
Sayyadi, Nima
Campbell, Douglas
Walsh, Bradley
Gillatt, David
Ebrahimi Warkiani, Majid
Zvyagin, Andrei V.
Rapid and Label-Free Isolation of Tumour Cells from the Urine of Patients with Localised Prostate Cancer Using Inertial Microfluidics
title Rapid and Label-Free Isolation of Tumour Cells from the Urine of Patients with Localised Prostate Cancer Using Inertial Microfluidics
title_full Rapid and Label-Free Isolation of Tumour Cells from the Urine of Patients with Localised Prostate Cancer Using Inertial Microfluidics
title_fullStr Rapid and Label-Free Isolation of Tumour Cells from the Urine of Patients with Localised Prostate Cancer Using Inertial Microfluidics
title_full_unstemmed Rapid and Label-Free Isolation of Tumour Cells from the Urine of Patients with Localised Prostate Cancer Using Inertial Microfluidics
title_short Rapid and Label-Free Isolation of Tumour Cells from the Urine of Patients with Localised Prostate Cancer Using Inertial Microfluidics
title_sort rapid and label-free isolation of tumour cells from the urine of patients with localised prostate cancer using inertial microfluidics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016827/
https://www.ncbi.nlm.nih.gov/pubmed/31905736
http://dx.doi.org/10.3390/cancers12010081
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