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Expression of Heat Shock Proteins in Thermally Challenged Pacific Abalone Haliotis discus hannai

Summer mortality, caused by thermal conditions, is the biggest threat to abalone aquaculture production industries. Various measures have been taken to mitigate this issue by adjusting the environment; however, the cellular processes of Pacific abalone (Haliotis discus hannai) have been overlooked d...

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Autores principales: Kyeong, Dongsoo, Kim, Juyeon, Shin, Younhee, Subramaniyam, Sathiyamoorthy, Kang, Byeong-Chul, Shin, Eun-Ha, Park, Eun Hee, Noh, Eun Soo, Kim, Young-Ok, Park, Jung Youn, Nam, Bo-Hye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016835/
https://www.ncbi.nlm.nih.gov/pubmed/31878084
http://dx.doi.org/10.3390/genes11010022
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author Kyeong, Dongsoo
Kim, Juyeon
Shin, Younhee
Subramaniyam, Sathiyamoorthy
Kang, Byeong-Chul
Shin, Eun-Ha
Park, Eun Hee
Noh, Eun Soo
Kim, Young-Ok
Park, Jung Youn
Nam, Bo-Hye
author_facet Kyeong, Dongsoo
Kim, Juyeon
Shin, Younhee
Subramaniyam, Sathiyamoorthy
Kang, Byeong-Chul
Shin, Eun-Ha
Park, Eun Hee
Noh, Eun Soo
Kim, Young-Ok
Park, Jung Youn
Nam, Bo-Hye
author_sort Kyeong, Dongsoo
collection PubMed
description Summer mortality, caused by thermal conditions, is the biggest threat to abalone aquaculture production industries. Various measures have been taken to mitigate this issue by adjusting the environment; however, the cellular processes of Pacific abalone (Haliotis discus hannai) have been overlooked due to the paucity of genetic information. The draft genome of H. discus hannai has recently been reported, prompting exploration of the genes responsible for thermal regulation in Pacific abalone. In this study, 413 proteins were systematically annotated as members of the heat shock protein (HSP) super families, and among them 26 HSP genes from four Pacific abalone tissues (hemocytes, gill, mantle, and muscle) were differentially expressed under cold and heat stress conditions. The co-expression network revealed that HSP expression patterns were tissue-specific and similar to those of other shellfish inhabiting intertidal zones. Finally, representative HSPs were selected at random and their expression patterns were identified by RNA sequencing and validated by qRT-PCR to assess expression significance. The HSPs expressed in hemocytes were highly similar in both analyses, suggesting that hemocytes could be more reliable samples for validating thermal condition markers compared to other tissues.
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spelling pubmed-70168352020-02-28 Expression of Heat Shock Proteins in Thermally Challenged Pacific Abalone Haliotis discus hannai Kyeong, Dongsoo Kim, Juyeon Shin, Younhee Subramaniyam, Sathiyamoorthy Kang, Byeong-Chul Shin, Eun-Ha Park, Eun Hee Noh, Eun Soo Kim, Young-Ok Park, Jung Youn Nam, Bo-Hye Genes (Basel) Article Summer mortality, caused by thermal conditions, is the biggest threat to abalone aquaculture production industries. Various measures have been taken to mitigate this issue by adjusting the environment; however, the cellular processes of Pacific abalone (Haliotis discus hannai) have been overlooked due to the paucity of genetic information. The draft genome of H. discus hannai has recently been reported, prompting exploration of the genes responsible for thermal regulation in Pacific abalone. In this study, 413 proteins were systematically annotated as members of the heat shock protein (HSP) super families, and among them 26 HSP genes from four Pacific abalone tissues (hemocytes, gill, mantle, and muscle) were differentially expressed under cold and heat stress conditions. The co-expression network revealed that HSP expression patterns were tissue-specific and similar to those of other shellfish inhabiting intertidal zones. Finally, representative HSPs were selected at random and their expression patterns were identified by RNA sequencing and validated by qRT-PCR to assess expression significance. The HSPs expressed in hemocytes were highly similar in both analyses, suggesting that hemocytes could be more reliable samples for validating thermal condition markers compared to other tissues. MDPI 2019-12-23 /pmc/articles/PMC7016835/ /pubmed/31878084 http://dx.doi.org/10.3390/genes11010022 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kyeong, Dongsoo
Kim, Juyeon
Shin, Younhee
Subramaniyam, Sathiyamoorthy
Kang, Byeong-Chul
Shin, Eun-Ha
Park, Eun Hee
Noh, Eun Soo
Kim, Young-Ok
Park, Jung Youn
Nam, Bo-Hye
Expression of Heat Shock Proteins in Thermally Challenged Pacific Abalone Haliotis discus hannai
title Expression of Heat Shock Proteins in Thermally Challenged Pacific Abalone Haliotis discus hannai
title_full Expression of Heat Shock Proteins in Thermally Challenged Pacific Abalone Haliotis discus hannai
title_fullStr Expression of Heat Shock Proteins in Thermally Challenged Pacific Abalone Haliotis discus hannai
title_full_unstemmed Expression of Heat Shock Proteins in Thermally Challenged Pacific Abalone Haliotis discus hannai
title_short Expression of Heat Shock Proteins in Thermally Challenged Pacific Abalone Haliotis discus hannai
title_sort expression of heat shock proteins in thermally challenged pacific abalone haliotis discus hannai
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016835/
https://www.ncbi.nlm.nih.gov/pubmed/31878084
http://dx.doi.org/10.3390/genes11010022
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