Cargando…

Circulating mucosal‐associated invariant T cells in subjects with recurrent urinary tract infections are functionally impaired

BACKGROUND: Urinary tract infection recurrence is common, particularly in women and immunocompromised patients, such as renal transplant recipients (RTRs). Mucosal‐associated invariant T (MAIT) cells play a role in the antibacterial response by recognizing bacterial riboflavin metabolites produced b...

Descripción completa

Detalles Bibliográficos
Autores principales: Terpstra, Matty L., Remmerswaal, Ester B. M., van Aalderen, Michiel C., Wever, Joyce J., Sinnige, Marjan J., van der Bom‐Baylon, Nelly D., Bemelman, Frederike J., Geerlings, Suzanne E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016840/
https://www.ncbi.nlm.nih.gov/pubmed/32032475
http://dx.doi.org/10.1002/iid3.287
_version_ 1783497067959681024
author Terpstra, Matty L.
Remmerswaal, Ester B. M.
van Aalderen, Michiel C.
Wever, Joyce J.
Sinnige, Marjan J.
van der Bom‐Baylon, Nelly D.
Bemelman, Frederike J.
Geerlings, Suzanne E.
author_facet Terpstra, Matty L.
Remmerswaal, Ester B. M.
van Aalderen, Michiel C.
Wever, Joyce J.
Sinnige, Marjan J.
van der Bom‐Baylon, Nelly D.
Bemelman, Frederike J.
Geerlings, Suzanne E.
author_sort Terpstra, Matty L.
collection PubMed
description BACKGROUND: Urinary tract infection recurrence is common, particularly in women and immunocompromised patients, such as renal transplant recipients (RTRs). Mucosal‐associated invariant T (MAIT) cells play a role in the antibacterial response by recognizing bacterial riboflavin metabolites produced by bacteria such as Escherichia coli. Here, we investigated whether MAIT cells are involved in the pathogenesis of recurrent urinary tract infections (RUTIs). METHODS: Using multichannel flow cytometry, we characterized the MAIT cell phenotype and function in blood from immunocompetent adults with (n = 13) and without RUTIs (n = 10) and in RTRs with (n = 9) and without RUTIs (n = 10). RESULTS: There were no differences in the numbers of MAIT cells between the study groups. MAIT cells in patients with RUTI expressed T‐bet more often than those in controls. MAIT cells from immunocompetent RUTI participants required more antigen‐presenting cells coincubated with E. coli to evoke a similar cytokine and degranulation response than those from controls. This effect was absent in the RTR with RUTI vs RTR control groups, where the overall percentage of MAIT cells that responded to stimulation was already reduced. CONCLUSION: Circulating MAIT cells in immunocompetent individuals with RUTIs respond to bacterial stimuli with reduced efficacy, which suggests that they are involved in the pathogenesis of RUTIs.
format Online
Article
Text
id pubmed-7016840
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-70168402020-03-06 Circulating mucosal‐associated invariant T cells in subjects with recurrent urinary tract infections are functionally impaired Terpstra, Matty L. Remmerswaal, Ester B. M. van Aalderen, Michiel C. Wever, Joyce J. Sinnige, Marjan J. van der Bom‐Baylon, Nelly D. Bemelman, Frederike J. Geerlings, Suzanne E. Immun Inflamm Dis Original Research BACKGROUND: Urinary tract infection recurrence is common, particularly in women and immunocompromised patients, such as renal transplant recipients (RTRs). Mucosal‐associated invariant T (MAIT) cells play a role in the antibacterial response by recognizing bacterial riboflavin metabolites produced by bacteria such as Escherichia coli. Here, we investigated whether MAIT cells are involved in the pathogenesis of recurrent urinary tract infections (RUTIs). METHODS: Using multichannel flow cytometry, we characterized the MAIT cell phenotype and function in blood from immunocompetent adults with (n = 13) and without RUTIs (n = 10) and in RTRs with (n = 9) and without RUTIs (n = 10). RESULTS: There were no differences in the numbers of MAIT cells between the study groups. MAIT cells in patients with RUTI expressed T‐bet more often than those in controls. MAIT cells from immunocompetent RUTI participants required more antigen‐presenting cells coincubated with E. coli to evoke a similar cytokine and degranulation response than those from controls. This effect was absent in the RTR with RUTI vs RTR control groups, where the overall percentage of MAIT cells that responded to stimulation was already reduced. CONCLUSION: Circulating MAIT cells in immunocompetent individuals with RUTIs respond to bacterial stimuli with reduced efficacy, which suggests that they are involved in the pathogenesis of RUTIs. John Wiley and Sons Inc. 2020-02-07 /pmc/articles/PMC7016840/ /pubmed/32032475 http://dx.doi.org/10.1002/iid3.287 Text en © 2020 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Terpstra, Matty L.
Remmerswaal, Ester B. M.
van Aalderen, Michiel C.
Wever, Joyce J.
Sinnige, Marjan J.
van der Bom‐Baylon, Nelly D.
Bemelman, Frederike J.
Geerlings, Suzanne E.
Circulating mucosal‐associated invariant T cells in subjects with recurrent urinary tract infections are functionally impaired
title Circulating mucosal‐associated invariant T cells in subjects with recurrent urinary tract infections are functionally impaired
title_full Circulating mucosal‐associated invariant T cells in subjects with recurrent urinary tract infections are functionally impaired
title_fullStr Circulating mucosal‐associated invariant T cells in subjects with recurrent urinary tract infections are functionally impaired
title_full_unstemmed Circulating mucosal‐associated invariant T cells in subjects with recurrent urinary tract infections are functionally impaired
title_short Circulating mucosal‐associated invariant T cells in subjects with recurrent urinary tract infections are functionally impaired
title_sort circulating mucosal‐associated invariant t cells in subjects with recurrent urinary tract infections are functionally impaired
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016840/
https://www.ncbi.nlm.nih.gov/pubmed/32032475
http://dx.doi.org/10.1002/iid3.287
work_keys_str_mv AT terpstramattyl circulatingmucosalassociatedinvarianttcellsinsubjectswithrecurrenturinarytractinfectionsarefunctionallyimpaired
AT remmerswaalesterbm circulatingmucosalassociatedinvarianttcellsinsubjectswithrecurrenturinarytractinfectionsarefunctionallyimpaired
AT vanaalderenmichielc circulatingmucosalassociatedinvarianttcellsinsubjectswithrecurrenturinarytractinfectionsarefunctionallyimpaired
AT weverjoycej circulatingmucosalassociatedinvarianttcellsinsubjectswithrecurrenturinarytractinfectionsarefunctionallyimpaired
AT sinnigemarjanj circulatingmucosalassociatedinvarianttcellsinsubjectswithrecurrenturinarytractinfectionsarefunctionallyimpaired
AT vanderbombaylonnellyd circulatingmucosalassociatedinvarianttcellsinsubjectswithrecurrenturinarytractinfectionsarefunctionallyimpaired
AT bemelmanfrederikej circulatingmucosalassociatedinvarianttcellsinsubjectswithrecurrenturinarytractinfectionsarefunctionallyimpaired
AT geerlingssuzannee circulatingmucosalassociatedinvarianttcellsinsubjectswithrecurrenturinarytractinfectionsarefunctionallyimpaired