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Toxicological Risks of Renqingchangjue in Rats Evaluated by (1)H NMR-Based Serum and Urine Metabolomics Analysis

[Image: see text] Renqingchangjue (RQCJ), a kind of Traditional Tibetan Medicine, has been widely utilized to treat various gastroenteritis diseases. However, the biosafety and toxicity of RQCJ was still indefinite because of toxic components in RQCJ, which included a variety of heavy metals. Thus,...

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Autores principales: Wang, Xia, Rezeng, Caidan, Wang, Yingfeng, Li, Jian, Zhang, Lan, Chen, Jianxin, Li, Zhongfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016918/
https://www.ncbi.nlm.nih.gov/pubmed/32064377
http://dx.doi.org/10.1021/acsomega.9b03084
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author Wang, Xia
Rezeng, Caidan
Wang, Yingfeng
Li, Jian
Zhang, Lan
Chen, Jianxin
Li, Zhongfeng
author_facet Wang, Xia
Rezeng, Caidan
Wang, Yingfeng
Li, Jian
Zhang, Lan
Chen, Jianxin
Li, Zhongfeng
author_sort Wang, Xia
collection PubMed
description [Image: see text] Renqingchangjue (RQCJ), a kind of Traditional Tibetan Medicine, has been widely utilized to treat various gastroenteritis diseases. However, the biosafety and toxicity of RQCJ was still indefinite because of toxic components in RQCJ, which included a variety of heavy metals. Thus, this study was aimed to evaluate the toxicity and expound the toxicological mechanism of RQCJ. In this study, rats were intragastrically administered with different doses of RQCJ for 15 days, and then, the restorative observation period lasted for 15 days. Liver and kidney tissues were collected for histopathological examination, and simultaneously serum and urine samples were collected for (1)H nuclear magnetic resonance ((1)H NMR) spectroscopy analysis and biochemical analysis combined with inductively coupled plasma mass spectrometry (ICP-MS) measurement. The (1)H NMR-based metabolomics analysis revealed that the administration of RQCJ significantly altered the concentrations of 14 serum metabolites and 14 urine metabolites, which implied disturbances in energy metabolism, amino acid metabolism, intestinal flora environment, and membrane damage. Besides, the biochemical analysis of serum samples was consistent with the histopathological results, which indicated slight hepatotoxicity and nephrotoxicity. The quantification of As and Hg in urine and serum samples by ICP-MS provided more evidence about the toxicity of RQCJ. This work provided an effective method to systematically and dynamically evaluate the toxicity of RQCJ and suggested that precautions should be taken in the clinic to monitor the potential toxicity of RQCJ.
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spelling pubmed-70169182020-02-14 Toxicological Risks of Renqingchangjue in Rats Evaluated by (1)H NMR-Based Serum and Urine Metabolomics Analysis Wang, Xia Rezeng, Caidan Wang, Yingfeng Li, Jian Zhang, Lan Chen, Jianxin Li, Zhongfeng ACS Omega [Image: see text] Renqingchangjue (RQCJ), a kind of Traditional Tibetan Medicine, has been widely utilized to treat various gastroenteritis diseases. However, the biosafety and toxicity of RQCJ was still indefinite because of toxic components in RQCJ, which included a variety of heavy metals. Thus, this study was aimed to evaluate the toxicity and expound the toxicological mechanism of RQCJ. In this study, rats were intragastrically administered with different doses of RQCJ for 15 days, and then, the restorative observation period lasted for 15 days. Liver and kidney tissues were collected for histopathological examination, and simultaneously serum and urine samples were collected for (1)H nuclear magnetic resonance ((1)H NMR) spectroscopy analysis and biochemical analysis combined with inductively coupled plasma mass spectrometry (ICP-MS) measurement. The (1)H NMR-based metabolomics analysis revealed that the administration of RQCJ significantly altered the concentrations of 14 serum metabolites and 14 urine metabolites, which implied disturbances in energy metabolism, amino acid metabolism, intestinal flora environment, and membrane damage. Besides, the biochemical analysis of serum samples was consistent with the histopathological results, which indicated slight hepatotoxicity and nephrotoxicity. The quantification of As and Hg in urine and serum samples by ICP-MS provided more evidence about the toxicity of RQCJ. This work provided an effective method to systematically and dynamically evaluate the toxicity of RQCJ and suggested that precautions should be taken in the clinic to monitor the potential toxicity of RQCJ. American Chemical Society 2020-01-28 /pmc/articles/PMC7016918/ /pubmed/32064377 http://dx.doi.org/10.1021/acsomega.9b03084 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Wang, Xia
Rezeng, Caidan
Wang, Yingfeng
Li, Jian
Zhang, Lan
Chen, Jianxin
Li, Zhongfeng
Toxicological Risks of Renqingchangjue in Rats Evaluated by (1)H NMR-Based Serum and Urine Metabolomics Analysis
title Toxicological Risks of Renqingchangjue in Rats Evaluated by (1)H NMR-Based Serum and Urine Metabolomics Analysis
title_full Toxicological Risks of Renqingchangjue in Rats Evaluated by (1)H NMR-Based Serum and Urine Metabolomics Analysis
title_fullStr Toxicological Risks of Renqingchangjue in Rats Evaluated by (1)H NMR-Based Serum and Urine Metabolomics Analysis
title_full_unstemmed Toxicological Risks of Renqingchangjue in Rats Evaluated by (1)H NMR-Based Serum and Urine Metabolomics Analysis
title_short Toxicological Risks of Renqingchangjue in Rats Evaluated by (1)H NMR-Based Serum and Urine Metabolomics Analysis
title_sort toxicological risks of renqingchangjue in rats evaluated by (1)h nmr-based serum and urine metabolomics analysis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016918/
https://www.ncbi.nlm.nih.gov/pubmed/32064377
http://dx.doi.org/10.1021/acsomega.9b03084
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