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Brain Invasion along Perivascular Spaces by Glioma Cells: Relationship with Blood–Brain Barrier

The question whether perivascular glioma cells invading the brain far from the tumor bulk may disrupt the blood–brain barrier (BBB) represents a crucial issue because under this condition tumor cells would be no more protected from the reach of chemotherapeutic drugs. A recent in vivo study that use...

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Autores principales: Pacioni, Simone, D’Alessandris, Quintino Giorgio, Buccarelli, Mariachiara, Boe, Alessandra, Martini, Maurizio, Larocca, Luigi Maria, Bolasco, Giulia, Ricci-Vitiani, Lucia, Falchetti, Maria Laura, Pallini, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7017006/
https://www.ncbi.nlm.nih.gov/pubmed/31861603
http://dx.doi.org/10.3390/cancers12010018
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author Pacioni, Simone
D’Alessandris, Quintino Giorgio
Buccarelli, Mariachiara
Boe, Alessandra
Martini, Maurizio
Larocca, Luigi Maria
Bolasco, Giulia
Ricci-Vitiani, Lucia
Falchetti, Maria Laura
Pallini, Roberto
author_facet Pacioni, Simone
D’Alessandris, Quintino Giorgio
Buccarelli, Mariachiara
Boe, Alessandra
Martini, Maurizio
Larocca, Luigi Maria
Bolasco, Giulia
Ricci-Vitiani, Lucia
Falchetti, Maria Laura
Pallini, Roberto
author_sort Pacioni, Simone
collection PubMed
description The question whether perivascular glioma cells invading the brain far from the tumor bulk may disrupt the blood–brain barrier (BBB) represents a crucial issue because under this condition tumor cells would be no more protected from the reach of chemotherapeutic drugs. A recent in vivo study that used human xenolines, demonstrated that single glioma cells migrating away from the tumor bulk are sufficient to breach the BBB. Here, we used brain xenografts of patient-derived glioma stem-like cells (GSCs) to show by immunostaining that in spite of massive perivascular invasion, BBB integrity was preserved in the majority of vessels located outside the tumor bulk. Interestingly, the tumor cells that invaded the brain for the longest distances traveled along vessels with retained BBB integrity. In surgical specimens of malignant glioma, the area of brain invasion showed several vessels with preserved BBB that were surrounded by tumor cells. On transmission electron microscopy, the cell inter-junctions and basal lamina of the brain endothelium were preserved even in conditions in which the tumor cells lay adjacently to blood vessels. In conclusion, BBB integrity associates with extensive perivascular invasion of glioma cells.
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spelling pubmed-70170062020-02-28 Brain Invasion along Perivascular Spaces by Glioma Cells: Relationship with Blood–Brain Barrier Pacioni, Simone D’Alessandris, Quintino Giorgio Buccarelli, Mariachiara Boe, Alessandra Martini, Maurizio Larocca, Luigi Maria Bolasco, Giulia Ricci-Vitiani, Lucia Falchetti, Maria Laura Pallini, Roberto Cancers (Basel) Article The question whether perivascular glioma cells invading the brain far from the tumor bulk may disrupt the blood–brain barrier (BBB) represents a crucial issue because under this condition tumor cells would be no more protected from the reach of chemotherapeutic drugs. A recent in vivo study that used human xenolines, demonstrated that single glioma cells migrating away from the tumor bulk are sufficient to breach the BBB. Here, we used brain xenografts of patient-derived glioma stem-like cells (GSCs) to show by immunostaining that in spite of massive perivascular invasion, BBB integrity was preserved in the majority of vessels located outside the tumor bulk. Interestingly, the tumor cells that invaded the brain for the longest distances traveled along vessels with retained BBB integrity. In surgical specimens of malignant glioma, the area of brain invasion showed several vessels with preserved BBB that were surrounded by tumor cells. On transmission electron microscopy, the cell inter-junctions and basal lamina of the brain endothelium were preserved even in conditions in which the tumor cells lay adjacently to blood vessels. In conclusion, BBB integrity associates with extensive perivascular invasion of glioma cells. MDPI 2019-12-19 /pmc/articles/PMC7017006/ /pubmed/31861603 http://dx.doi.org/10.3390/cancers12010018 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pacioni, Simone
D’Alessandris, Quintino Giorgio
Buccarelli, Mariachiara
Boe, Alessandra
Martini, Maurizio
Larocca, Luigi Maria
Bolasco, Giulia
Ricci-Vitiani, Lucia
Falchetti, Maria Laura
Pallini, Roberto
Brain Invasion along Perivascular Spaces by Glioma Cells: Relationship with Blood–Brain Barrier
title Brain Invasion along Perivascular Spaces by Glioma Cells: Relationship with Blood–Brain Barrier
title_full Brain Invasion along Perivascular Spaces by Glioma Cells: Relationship with Blood–Brain Barrier
title_fullStr Brain Invasion along Perivascular Spaces by Glioma Cells: Relationship with Blood–Brain Barrier
title_full_unstemmed Brain Invasion along Perivascular Spaces by Glioma Cells: Relationship with Blood–Brain Barrier
title_short Brain Invasion along Perivascular Spaces by Glioma Cells: Relationship with Blood–Brain Barrier
title_sort brain invasion along perivascular spaces by glioma cells: relationship with blood–brain barrier
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7017006/
https://www.ncbi.nlm.nih.gov/pubmed/31861603
http://dx.doi.org/10.3390/cancers12010018
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