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The secretome of Pichia pastoris in fed‐batch cultivations is largely independent of the carbon source but changes quantitatively over cultivation time
The quantitative changes of the secretome of recombinant Pichia pastoris (Komagataella phaffii) CBS7435 over the time‐course of methanol‐ or glucose‐limited fed‐batch cultures were investigated by LC‐ESI‐MS/MS to define the carbon source‐specific secretomes under controlled bioreactor conditions. In...
Autores principales: | Burgard, Jonas, Grünwald‐Gruber, Clemens, Altmann, Friedrich, Zanghellini, Jürgen, Valli, Minoska, Mattanovich, Diethard, Gasser, Brigitte |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7017826/ https://www.ncbi.nlm.nih.gov/pubmed/31692260 http://dx.doi.org/10.1111/1751-7915.13499 |
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