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An improved strategy for CRISPR/Cas9 gene knockout and subsequent wildtype and mutant gene rescue

A fluorescence marker mOrange was inserted to the popular pLentiCrispr-V2 to create pLentiCrispr-V2-mOrange (V2mO) that contained both a puromycin selection and a fluorescent marker, making viral production and target transduction visible. Lentiviruses packaged with this plasmid and appropriate guid...

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Detalles Bibliográficos
Autores principales:	Jin, Jiankang, Xu, Yan, Huo, Longfei, Ma, Lang, Scott, Ailing W., Pizzi, Melissa Pool, Li, Yuan, Wang, Ying, Yao, Xiaodan, Song, Shumei, Ajani, Jaffer A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2020
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7018052/ https://www.ncbi.nlm.nih.gov/pubmed/32053639 http://dx.doi.org/10.1371/journal.pone.0228910

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