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MiR-9 promotes the phenotypic switch of vascular smooth muscle cells by targeting KLF5
BACKGROUND/AIM: Diabetic vascular smooth muscle cells (VSMCs) are characterized by increased proliferation and migration. Small noncoding microRNAs (miRNAs) have been considered critical modulators of the VSMC phenotypic switch after an environmental stimulus. However, microRNA in high glucose-induc...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Scientific and Technological Research Council of Turkey
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7018344/ https://www.ncbi.nlm.nih.gov/pubmed/31122000 http://dx.doi.org/10.3906/sag-1710-173 |
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author | LU, Xiaochun MA, Shi-Tang ZHOU, Bo LI, Tieling |
author_facet | LU, Xiaochun MA, Shi-Tang ZHOU, Bo LI, Tieling |
author_sort | LU, Xiaochun |
collection | PubMed |
description | BACKGROUND/AIM: Diabetic vascular smooth muscle cells (VSMCs) are characterized by increased proliferation and migration. Small noncoding microRNAs (miRNAs) have been considered critical modulators of the VSMC phenotypic switch after an environmental stimulus. However, microRNA in high glucose-induced proinflammation and its atherogenic effect is still ambiguous. MATERIALS AND METHODS: The technique of qRT-PCR was used to examine the expression of miR-9 in VSMCs. The downstream signaling protein relative to miR-9 regulation, Krüppel-like factor 5, and some marker genes of contractile VSMCs were analyzed by western blotting and qRT-PCR. Luciferase reporter assay was used to detect the expression of KLF5, which is regulated by miR-9. To examine the function of a miR-9 inhibitor in VSMC proliferation and migration, VSMC proliferation and migration assays were performed. RESULTS: Reduced transcriptional levels of miR-9 and expression of specific genes of contractile VSMCs were observed in the SMC cell line C-12511 treated with high glucose and SMCs, which were isolated from db/db mice. Moreover, the activity of KLF5 3′-UTR was dramatically reduced by a miR-9 mimic and increased by a miR-9 inhibitor. The proliferation and migration of SMCs were reduced by the miR-9 mimic. CONCLUSION: miR-9 inhibits the proliferation and migration of SMC by targeting KLF5 in db/db mice. |
format | Online Article Text |
id | pubmed-7018344 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Scientific and Technological Research Council of Turkey |
record_format | MEDLINE/PubMed |
spelling | pubmed-70183442020-03-23 MiR-9 promotes the phenotypic switch of vascular smooth muscle cells by targeting KLF5 LU, Xiaochun MA, Shi-Tang ZHOU, Bo LI, Tieling Turk J Med Sci Article BACKGROUND/AIM: Diabetic vascular smooth muscle cells (VSMCs) are characterized by increased proliferation and migration. Small noncoding microRNAs (miRNAs) have been considered critical modulators of the VSMC phenotypic switch after an environmental stimulus. However, microRNA in high glucose-induced proinflammation and its atherogenic effect is still ambiguous. MATERIALS AND METHODS: The technique of qRT-PCR was used to examine the expression of miR-9 in VSMCs. The downstream signaling protein relative to miR-9 regulation, Krüppel-like factor 5, and some marker genes of contractile VSMCs were analyzed by western blotting and qRT-PCR. Luciferase reporter assay was used to detect the expression of KLF5, which is regulated by miR-9. To examine the function of a miR-9 inhibitor in VSMC proliferation and migration, VSMC proliferation and migration assays were performed. RESULTS: Reduced transcriptional levels of miR-9 and expression of specific genes of contractile VSMCs were observed in the SMC cell line C-12511 treated with high glucose and SMCs, which were isolated from db/db mice. Moreover, the activity of KLF5 3′-UTR was dramatically reduced by a miR-9 mimic and increased by a miR-9 inhibitor. The proliferation and migration of SMCs were reduced by the miR-9 mimic. CONCLUSION: miR-9 inhibits the proliferation and migration of SMC by targeting KLF5 in db/db mice. The Scientific and Technological Research Council of Turkey 2019-06-18 /pmc/articles/PMC7018344/ /pubmed/31122000 http://dx.doi.org/10.3906/sag-1710-173 Text en Copyright © 2019 The Author(s) This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Article LU, Xiaochun MA, Shi-Tang ZHOU, Bo LI, Tieling MiR-9 promotes the phenotypic switch of vascular smooth muscle cells by targeting KLF5 |
title | MiR-9 promotes the phenotypic switch of vascular smooth muscle cells by targeting KLF5 |
title_full | MiR-9 promotes the phenotypic switch of vascular smooth muscle cells by targeting KLF5 |
title_fullStr | MiR-9 promotes the phenotypic switch of vascular smooth muscle cells by targeting KLF5 |
title_full_unstemmed | MiR-9 promotes the phenotypic switch of vascular smooth muscle cells by targeting KLF5 |
title_short | MiR-9 promotes the phenotypic switch of vascular smooth muscle cells by targeting KLF5 |
title_sort | mir-9 promotes the phenotypic switch of vascular smooth muscle cells by targeting klf5 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7018344/ https://www.ncbi.nlm.nih.gov/pubmed/31122000 http://dx.doi.org/10.3906/sag-1710-173 |
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