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Is there any difference between endometrial hyperplasia and endometrial carcinoma in terms of expression of TRPM2 and TRPM7 ion channels?
BACKGROUND/AIM: This study compared TRPM2 and TRPM7 ion channel gene expression and immunohistochemical staining in endometrial hyperplasia and endometrium adenocarcinoma. MATERIALS AND METHODS: Sections were taken from paraffin blocks of 120 patients who were divided into 6 groups as follows: G1 (n...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Scientific and Technological Research Council of Turkey
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7018370/ https://www.ncbi.nlm.nih.gov/pubmed/30997980 http://dx.doi.org/10.3906/sag-1810-176 |
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author | YALÇIN, Emre PALA, Şehmus ATILGAN*, Remzi KULOĞLU, Tuncay ÖNALAN, Ebru ARTAŞ, Gökhan BURAN, İlay |
author_facet | YALÇIN, Emre PALA, Şehmus ATILGAN*, Remzi KULOĞLU, Tuncay ÖNALAN, Ebru ARTAŞ, Gökhan BURAN, İlay |
author_sort | YALÇIN, Emre |
collection | PubMed |
description | BACKGROUND/AIM: This study compared TRPM2 and TRPM7 ion channel gene expression and immunohistochemical staining in endometrial hyperplasia and endometrium adenocarcinoma. MATERIALS AND METHODS: Sections were taken from paraffin blocks of 120 patients who were divided into 6 groups as follows: G1 (n = 20), proliferative endometrium (PE); G2 (n = 20), EH without atypia; G3 (n = 20), EH with atypia; G4 (n = 20), stage 1A, grade 1 EC; G5 (n = 20), stage 1A, grade 2 EC; and G6 (n = 20), stage 1A, grade 3 EC. TRPM2 and TRPM7 genes were analyzed with qRT-PCR in paraffin-embedded tissue samples. Under light microscopy, TRPM2 and TRPM7 immunostaining scores of the samples taken from polylysine slides were evaluated. RESULTS: Compared to G1, TRPM2 mRNA gene expression was significantly downregulated in G3 and G5. TRPM2 immunoreactivity scores were similar in all groups. TRPM7 mRNA gene expression was significantly downregulated in G2, G3, and G6 when compared to G1. TRPM7 immunoreactivity scores were similar in G1, G2, and G3, but significantly decreased in G4, G5, and G6. CONCLUSION: Reduction in TRPM7 ion channel activity may be a progression marker for endometrial hyperplasia regardless of the atypical criteria. |
format | Online Article Text |
id | pubmed-7018370 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Scientific and Technological Research Council of Turkey |
record_format | MEDLINE/PubMed |
spelling | pubmed-70183702020-03-23 Is there any difference between endometrial hyperplasia and endometrial carcinoma in terms of expression of TRPM2 and TRPM7 ion channels? YALÇIN, Emre PALA, Şehmus ATILGAN*, Remzi KULOĞLU, Tuncay ÖNALAN, Ebru ARTAŞ, Gökhan BURAN, İlay Turk J Med Sci Article BACKGROUND/AIM: This study compared TRPM2 and TRPM7 ion channel gene expression and immunohistochemical staining in endometrial hyperplasia and endometrium adenocarcinoma. MATERIALS AND METHODS: Sections were taken from paraffin blocks of 120 patients who were divided into 6 groups as follows: G1 (n = 20), proliferative endometrium (PE); G2 (n = 20), EH without atypia; G3 (n = 20), EH with atypia; G4 (n = 20), stage 1A, grade 1 EC; G5 (n = 20), stage 1A, grade 2 EC; and G6 (n = 20), stage 1A, grade 3 EC. TRPM2 and TRPM7 genes were analyzed with qRT-PCR in paraffin-embedded tissue samples. Under light microscopy, TRPM2 and TRPM7 immunostaining scores of the samples taken from polylysine slides were evaluated. RESULTS: Compared to G1, TRPM2 mRNA gene expression was significantly downregulated in G3 and G5. TRPM2 immunoreactivity scores were similar in all groups. TRPM7 mRNA gene expression was significantly downregulated in G2, G3, and G6 when compared to G1. TRPM7 immunoreactivity scores were similar in G1, G2, and G3, but significantly decreased in G4, G5, and G6. CONCLUSION: Reduction in TRPM7 ion channel activity may be a progression marker for endometrial hyperplasia regardless of the atypical criteria. The Scientific and Technological Research Council of Turkey 2019-04-18 /pmc/articles/PMC7018370/ /pubmed/30997980 http://dx.doi.org/10.3906/sag-1810-176 Text en Copyright © 2019 The Author(s) This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Article YALÇIN, Emre PALA, Şehmus ATILGAN*, Remzi KULOĞLU, Tuncay ÖNALAN, Ebru ARTAŞ, Gökhan BURAN, İlay Is there any difference between endometrial hyperplasia and endometrial carcinoma in terms of expression of TRPM2 and TRPM7 ion channels? |
title | Is there any difference between endometrial hyperplasia and endometrial carcinoma in terms of expression of TRPM2 and TRPM7 ion channels? |
title_full | Is there any difference between endometrial hyperplasia and endometrial carcinoma in terms of expression of TRPM2 and TRPM7 ion channels? |
title_fullStr | Is there any difference between endometrial hyperplasia and endometrial carcinoma in terms of expression of TRPM2 and TRPM7 ion channels? |
title_full_unstemmed | Is there any difference between endometrial hyperplasia and endometrial carcinoma in terms of expression of TRPM2 and TRPM7 ion channels? |
title_short | Is there any difference between endometrial hyperplasia and endometrial carcinoma in terms of expression of TRPM2 and TRPM7 ion channels? |
title_sort | is there any difference between endometrial hyperplasia and endometrial carcinoma in terms of expression of trpm2 and trpm7 ion channels? |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7018370/ https://www.ncbi.nlm.nih.gov/pubmed/30997980 http://dx.doi.org/10.3906/sag-1810-176 |
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