Isolation and genomic analysis of 11-aminoundecanoic acid-degrading bacterium Pseudomonas sp. JG-B from nylon 11 enrichment culture

Nylon 11 is a polymer synthesized from 11-aminoundecanoic acid, and widely used in commercial manufacturing. In this study, we describe the isolation of the first organism capable of metabolizing 11-aminoundecanoic acid from nylon 11 enrichment culture. The strain shows rapid growth on 11-aminoundec...

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Autores principales: Gatz-Schrupp, Jocelyn, Deckard, Peter, Hufford, Benjamin, Ly, Steven, Tupa, Peter, Masuda, Hisako
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2020
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019077/
https://www.ncbi.nlm.nih.gov/pubmed/32064005
http://dx.doi.org/10.7150/jgen.42095
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author Gatz-Schrupp, Jocelyn
Deckard, Peter
Hufford, Benjamin
Ly, Steven
Tupa, Peter
Masuda, Hisako
author_facet Gatz-Schrupp, Jocelyn
Deckard, Peter
Hufford, Benjamin
Ly, Steven
Tupa, Peter
Masuda, Hisako
author_sort Gatz-Schrupp, Jocelyn
collection PubMed
description Nylon 11 is a polymer synthesized from 11-aminoundecanoic acid, and widely used in commercial manufacturing. In this study, we describe the isolation of the first organism capable of metabolizing 11-aminoundecanoic acid from nylon 11 enrichment culture. The strain shows rapid growth on 11-aminoundecanoic acid as a sole source of carbon, nitrogen, and energy. Furthermore, the genome sequence of strain JG-B was deciphered and shown to belong to genus Pseudomonas. Many genes encoding putative extracellular hydrolases, as well as homologues of nylon 6 hydrolases (NylB and NylA) were identified, suggesting the metabolic versatility and possibility that this organism could also depolymerase nylon 11 polymers.
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spelling pubmed-70190772020-02-14 Isolation and genomic analysis of 11-aminoundecanoic acid-degrading bacterium Pseudomonas sp. JG-B from nylon 11 enrichment culture Gatz-Schrupp, Jocelyn Deckard, Peter Hufford, Benjamin Ly, Steven Tupa, Peter Masuda, Hisako J Genomics Research Paper Nylon 11 is a polymer synthesized from 11-aminoundecanoic acid, and widely used in commercial manufacturing. In this study, we describe the isolation of the first organism capable of metabolizing 11-aminoundecanoic acid from nylon 11 enrichment culture. The strain shows rapid growth on 11-aminoundecanoic acid as a sole source of carbon, nitrogen, and energy. Furthermore, the genome sequence of strain JG-B was deciphered and shown to belong to genus Pseudomonas. Many genes encoding putative extracellular hydrolases, as well as homologues of nylon 6 hydrolases (NylB and NylA) were identified, suggesting the metabolic versatility and possibility that this organism could also depolymerase nylon 11 polymers. Ivyspring International Publisher 2020-01-25 /pmc/articles/PMC7019077/ /pubmed/32064005 http://dx.doi.org/10.7150/jgen.42095 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Gatz-Schrupp, Jocelyn
Deckard, Peter
Hufford, Benjamin
Ly, Steven
Tupa, Peter
Masuda, Hisako
Isolation and genomic analysis of 11-aminoundecanoic acid-degrading bacterium Pseudomonas sp. JG-B from nylon 11 enrichment culture
title Isolation and genomic analysis of 11-aminoundecanoic acid-degrading bacterium Pseudomonas sp. JG-B from nylon 11 enrichment culture
title_full Isolation and genomic analysis of 11-aminoundecanoic acid-degrading bacterium Pseudomonas sp. JG-B from nylon 11 enrichment culture
title_fullStr Isolation and genomic analysis of 11-aminoundecanoic acid-degrading bacterium Pseudomonas sp. JG-B from nylon 11 enrichment culture
title_full_unstemmed Isolation and genomic analysis of 11-aminoundecanoic acid-degrading bacterium Pseudomonas sp. JG-B from nylon 11 enrichment culture
title_short Isolation and genomic analysis of 11-aminoundecanoic acid-degrading bacterium Pseudomonas sp. JG-B from nylon 11 enrichment culture
title_sort isolation and genomic analysis of 11-aminoundecanoic acid-degrading bacterium pseudomonas sp. jg-b from nylon 11 enrichment culture
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019077/
https://www.ncbi.nlm.nih.gov/pubmed/32064005
http://dx.doi.org/10.7150/jgen.42095
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