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PEGylated Bilirubin-coated Iron Oxide Nanoparticles as a Biosensor for Magnetic Relaxation Switching-based ROS Detection in Whole Blood

Rationale: Magnetic relaxation switching (MRSw) induced by target-triggered aggregation or dissociation of superparamagnetic iron oxide nanoparticles (SPIONs) have been utilized for detection of diverse biomarkers. However, an MRSw-based biosensor for reactive oxygen species (ROS) has never been doc...

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Autores principales: Lee, Dong Yun, Kang, Sukmo, Lee, Yonghyun, Kim, Jin Yong, Yoo, Dohyun, Jung, Wonsik, Lee, Soyoung, Jeong, Yong Yeon, Lee, Kwangyeol, Jon, Sangyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019166/
https://www.ncbi.nlm.nih.gov/pubmed/32104497
http://dx.doi.org/10.7150/thno.39662
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author Lee, Dong Yun
Kang, Sukmo
Lee, Yonghyun
Kim, Jin Yong
Yoo, Dohyun
Jung, Wonsik
Lee, Soyoung
Jeong, Yong Yeon
Lee, Kwangyeol
Jon, Sangyong
author_facet Lee, Dong Yun
Kang, Sukmo
Lee, Yonghyun
Kim, Jin Yong
Yoo, Dohyun
Jung, Wonsik
Lee, Soyoung
Jeong, Yong Yeon
Lee, Kwangyeol
Jon, Sangyong
author_sort Lee, Dong Yun
collection PubMed
description Rationale: Magnetic relaxation switching (MRSw) induced by target-triggered aggregation or dissociation of superparamagnetic iron oxide nanoparticles (SPIONs) have been utilized for detection of diverse biomarkers. However, an MRSw-based biosensor for reactive oxygen species (ROS) has never been documented. Methods: To this end, we constructed a biosensor for ROS detection based on PEGylated bilirubin (PEG-BR)-coated SPIONs (PEG-BR@SPIONs) that were prepared by simple sonication via ligand exchange. In addition, near infra-red (NIR) fluorescent dye was loaded onto PEG-BR@SPIONs as a secondary option for fluorescence-based ROS detection. Results: PEG-BR@SPIONs showed high colloidal stability under physiological conditions, but upon exposure to the model ROS, NaOCl, in vitro, they aggregated, causing a decrease in signal intensity in T2-weighted MR images. Furthermore, ROS-responsive PEG-BR@SPIONs were taken up by lipopolysaccharide (LPS)-activated macrophages to a much greater extent than ROS-unresponsive control nanoparticles (PEG-DSPE@SPIONs). In a sepsis-mimetic clinical setting, PEG-BR@SPIONs were able to directly detect the concentrations of ROS in whole blood samples through a clear change in T2 MR signals and a 'turn-on' signal of fluorescence. Conclusions: These findings suggest that PEG-BR@SPIONs have the potential as a new type of dual mode (MRSw-based and fluorescence-based) biosensors for ROS detection and could be used to diagnose many diseases associated with ROS overproduction.
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spelling pubmed-70191662020-02-26 PEGylated Bilirubin-coated Iron Oxide Nanoparticles as a Biosensor for Magnetic Relaxation Switching-based ROS Detection in Whole Blood Lee, Dong Yun Kang, Sukmo Lee, Yonghyun Kim, Jin Yong Yoo, Dohyun Jung, Wonsik Lee, Soyoung Jeong, Yong Yeon Lee, Kwangyeol Jon, Sangyong Theranostics Research Paper Rationale: Magnetic relaxation switching (MRSw) induced by target-triggered aggregation or dissociation of superparamagnetic iron oxide nanoparticles (SPIONs) have been utilized for detection of diverse biomarkers. However, an MRSw-based biosensor for reactive oxygen species (ROS) has never been documented. Methods: To this end, we constructed a biosensor for ROS detection based on PEGylated bilirubin (PEG-BR)-coated SPIONs (PEG-BR@SPIONs) that were prepared by simple sonication via ligand exchange. In addition, near infra-red (NIR) fluorescent dye was loaded onto PEG-BR@SPIONs as a secondary option for fluorescence-based ROS detection. Results: PEG-BR@SPIONs showed high colloidal stability under physiological conditions, but upon exposure to the model ROS, NaOCl, in vitro, they aggregated, causing a decrease in signal intensity in T2-weighted MR images. Furthermore, ROS-responsive PEG-BR@SPIONs were taken up by lipopolysaccharide (LPS)-activated macrophages to a much greater extent than ROS-unresponsive control nanoparticles (PEG-DSPE@SPIONs). In a sepsis-mimetic clinical setting, PEG-BR@SPIONs were able to directly detect the concentrations of ROS in whole blood samples through a clear change in T2 MR signals and a 'turn-on' signal of fluorescence. Conclusions: These findings suggest that PEG-BR@SPIONs have the potential as a new type of dual mode (MRSw-based and fluorescence-based) biosensors for ROS detection and could be used to diagnose many diseases associated with ROS overproduction. Ivyspring International Publisher 2020-01-12 /pmc/articles/PMC7019166/ /pubmed/32104497 http://dx.doi.org/10.7150/thno.39662 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Lee, Dong Yun
Kang, Sukmo
Lee, Yonghyun
Kim, Jin Yong
Yoo, Dohyun
Jung, Wonsik
Lee, Soyoung
Jeong, Yong Yeon
Lee, Kwangyeol
Jon, Sangyong
PEGylated Bilirubin-coated Iron Oxide Nanoparticles as a Biosensor for Magnetic Relaxation Switching-based ROS Detection in Whole Blood
title PEGylated Bilirubin-coated Iron Oxide Nanoparticles as a Biosensor for Magnetic Relaxation Switching-based ROS Detection in Whole Blood
title_full PEGylated Bilirubin-coated Iron Oxide Nanoparticles as a Biosensor for Magnetic Relaxation Switching-based ROS Detection in Whole Blood
title_fullStr PEGylated Bilirubin-coated Iron Oxide Nanoparticles as a Biosensor for Magnetic Relaxation Switching-based ROS Detection in Whole Blood
title_full_unstemmed PEGylated Bilirubin-coated Iron Oxide Nanoparticles as a Biosensor for Magnetic Relaxation Switching-based ROS Detection in Whole Blood
title_short PEGylated Bilirubin-coated Iron Oxide Nanoparticles as a Biosensor for Magnetic Relaxation Switching-based ROS Detection in Whole Blood
title_sort pegylated bilirubin-coated iron oxide nanoparticles as a biosensor for magnetic relaxation switching-based ros detection in whole blood
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019166/
https://www.ncbi.nlm.nih.gov/pubmed/32104497
http://dx.doi.org/10.7150/thno.39662
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