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The Deoptimization of Rabies Virus Matrix Protein Impacts Viral Transcription and Replication
Rabies virus (RABV) matrix (M) protein plays several important roles during RABV infection. Although previous studies have assessed the functions of M through gene rearrangements, this interferes with the position of other viral proteins. In this study, we attenuated M expression through deoptimizin...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019236/ https://www.ncbi.nlm.nih.gov/pubmed/31861477 http://dx.doi.org/10.3390/v12010004 |
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author | Luo, Jun Zhang, Yue Zhang, Qiong Wu, Yuting Zhang, Boyue Mo, Meijun Tian, Qin Zhao, Jing Mei, Mingzhu Guo, Xiaofeng |
author_facet | Luo, Jun Zhang, Yue Zhang, Qiong Wu, Yuting Zhang, Boyue Mo, Meijun Tian, Qin Zhao, Jing Mei, Mingzhu Guo, Xiaofeng |
author_sort | Luo, Jun |
collection | PubMed |
description | Rabies virus (RABV) matrix (M) protein plays several important roles during RABV infection. Although previous studies have assessed the functions of M through gene rearrangements, this interferes with the position of other viral proteins. In this study, we attenuated M expression through deoptimizing its codon usage based on codon pair bias in RABV. This strategy more objectively clarifies the role of M during virus infection. Codon-deoptimized M inhibited RABV replication during the early stages of infection, but enhanced viral titers at later stages. Codon-deoptimized M also inhibited genome synthesis at early stage of infection and increased the RABV transcription rates. Attenuated M through codon deoptimization enhanced RABV glycoprotein expression following RABV infection in neuronal cells, but had no influence on the cell-to-cell spread of RABV. In addition, codon-deoptimized M virus induced higher levels of apoptosis compared to the parental RABV. These results indicate that codon-deoptimized M increases glycoprotein expression, providing a foundation for further investigation of the role of M during RABV infection. |
format | Online Article Text |
id | pubmed-7019236 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70192362020-03-04 The Deoptimization of Rabies Virus Matrix Protein Impacts Viral Transcription and Replication Luo, Jun Zhang, Yue Zhang, Qiong Wu, Yuting Zhang, Boyue Mo, Meijun Tian, Qin Zhao, Jing Mei, Mingzhu Guo, Xiaofeng Viruses Article Rabies virus (RABV) matrix (M) protein plays several important roles during RABV infection. Although previous studies have assessed the functions of M through gene rearrangements, this interferes with the position of other viral proteins. In this study, we attenuated M expression through deoptimizing its codon usage based on codon pair bias in RABV. This strategy more objectively clarifies the role of M during virus infection. Codon-deoptimized M inhibited RABV replication during the early stages of infection, but enhanced viral titers at later stages. Codon-deoptimized M also inhibited genome synthesis at early stage of infection and increased the RABV transcription rates. Attenuated M through codon deoptimization enhanced RABV glycoprotein expression following RABV infection in neuronal cells, but had no influence on the cell-to-cell spread of RABV. In addition, codon-deoptimized M virus induced higher levels of apoptosis compared to the parental RABV. These results indicate that codon-deoptimized M increases glycoprotein expression, providing a foundation for further investigation of the role of M during RABV infection. MDPI 2019-12-18 /pmc/articles/PMC7019236/ /pubmed/31861477 http://dx.doi.org/10.3390/v12010004 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Luo, Jun Zhang, Yue Zhang, Qiong Wu, Yuting Zhang, Boyue Mo, Meijun Tian, Qin Zhao, Jing Mei, Mingzhu Guo, Xiaofeng The Deoptimization of Rabies Virus Matrix Protein Impacts Viral Transcription and Replication |
title | The Deoptimization of Rabies Virus Matrix Protein Impacts Viral Transcription and Replication |
title_full | The Deoptimization of Rabies Virus Matrix Protein Impacts Viral Transcription and Replication |
title_fullStr | The Deoptimization of Rabies Virus Matrix Protein Impacts Viral Transcription and Replication |
title_full_unstemmed | The Deoptimization of Rabies Virus Matrix Protein Impacts Viral Transcription and Replication |
title_short | The Deoptimization of Rabies Virus Matrix Protein Impacts Viral Transcription and Replication |
title_sort | deoptimization of rabies virus matrix protein impacts viral transcription and replication |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019236/ https://www.ncbi.nlm.nih.gov/pubmed/31861477 http://dx.doi.org/10.3390/v12010004 |
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