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Dye-Doped ZnO Microcapsules for High Throughput and Sensitive Optofluidic Micro-Thermometry

The main objective of this work is to show the proof of concept of a new optofluidic method for high throughput fluorescence-based thermometry, which enables the measure of temperature inside optofluidic microsystems at the millisecond (ms) time scale (high throughput). We used droplet microfluidics...

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Autores principales: Ghifari, Najla, Rassouk, Sara, Hayat, Zain, Taleb, Abdelhafed, Chahboun, Adil, El Abed, Abdel I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019242/
https://www.ncbi.nlm.nih.gov/pubmed/31963439
http://dx.doi.org/10.3390/mi11010100
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author Ghifari, Najla
Rassouk, Sara
Hayat, Zain
Taleb, Abdelhafed
Chahboun, Adil
El Abed, Abdel I.
author_facet Ghifari, Najla
Rassouk, Sara
Hayat, Zain
Taleb, Abdelhafed
Chahboun, Adil
El Abed, Abdel I.
author_sort Ghifari, Najla
collection PubMed
description The main objective of this work is to show the proof of concept of a new optofluidic method for high throughput fluorescence-based thermometry, which enables the measure of temperature inside optofluidic microsystems at the millisecond (ms) time scale (high throughput). We used droplet microfluidics to produce highly monodisperse microspheres from dispersed zinc oxide (ZnO) nanocrystals and doped them with rhodamine B (RhB) or/and rhodamine 6G (Rh6G). The fluorescence intensities of these two dyes are known to depend linearly on temperature but in two opposite manner. Their mixture enables for the construction of reference probe whose fluorescence does not depend practically on temperature. The use of zinc oxide microparticles as temperature probes in microfluidic channels has two main advantages: (i) avoid the diffusion and the adsorption of the dyes inside the walls of the microfluidic channels and (ii) enhance dissipation of the heat generated by the focused incident laser beam thanks to the high thermal conductivity of this material. Our results show that the fluorescence intensity of RhB decreases linearly with increasing temperature at a rate of about −2.2%/°C, in a very good agreement with the literature. In contrast, we observed for the first time a nonlinear change of the fluorescence intensity of Rh6G in ZnO microparticles with a minimum intensity at a temperature equal to 40 °C. This behaviour is reproducible and was observed only with ZnO microparticles doped with Rh6G.
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spelling pubmed-70192422020-03-04 Dye-Doped ZnO Microcapsules for High Throughput and Sensitive Optofluidic Micro-Thermometry Ghifari, Najla Rassouk, Sara Hayat, Zain Taleb, Abdelhafed Chahboun, Adil El Abed, Abdel I. Micromachines (Basel) Article The main objective of this work is to show the proof of concept of a new optofluidic method for high throughput fluorescence-based thermometry, which enables the measure of temperature inside optofluidic microsystems at the millisecond (ms) time scale (high throughput). We used droplet microfluidics to produce highly monodisperse microspheres from dispersed zinc oxide (ZnO) nanocrystals and doped them with rhodamine B (RhB) or/and rhodamine 6G (Rh6G). The fluorescence intensities of these two dyes are known to depend linearly on temperature but in two opposite manner. Their mixture enables for the construction of reference probe whose fluorescence does not depend practically on temperature. The use of zinc oxide microparticles as temperature probes in microfluidic channels has two main advantages: (i) avoid the diffusion and the adsorption of the dyes inside the walls of the microfluidic channels and (ii) enhance dissipation of the heat generated by the focused incident laser beam thanks to the high thermal conductivity of this material. Our results show that the fluorescence intensity of RhB decreases linearly with increasing temperature at a rate of about −2.2%/°C, in a very good agreement with the literature. In contrast, we observed for the first time a nonlinear change of the fluorescence intensity of Rh6G in ZnO microparticles with a minimum intensity at a temperature equal to 40 °C. This behaviour is reproducible and was observed only with ZnO microparticles doped with Rh6G. MDPI 2020-01-17 /pmc/articles/PMC7019242/ /pubmed/31963439 http://dx.doi.org/10.3390/mi11010100 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ghifari, Najla
Rassouk, Sara
Hayat, Zain
Taleb, Abdelhafed
Chahboun, Adil
El Abed, Abdel I.
Dye-Doped ZnO Microcapsules for High Throughput and Sensitive Optofluidic Micro-Thermometry
title Dye-Doped ZnO Microcapsules for High Throughput and Sensitive Optofluidic Micro-Thermometry
title_full Dye-Doped ZnO Microcapsules for High Throughput and Sensitive Optofluidic Micro-Thermometry
title_fullStr Dye-Doped ZnO Microcapsules for High Throughput and Sensitive Optofluidic Micro-Thermometry
title_full_unstemmed Dye-Doped ZnO Microcapsules for High Throughput and Sensitive Optofluidic Micro-Thermometry
title_short Dye-Doped ZnO Microcapsules for High Throughput and Sensitive Optofluidic Micro-Thermometry
title_sort dye-doped zno microcapsules for high throughput and sensitive optofluidic micro-thermometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019242/
https://www.ncbi.nlm.nih.gov/pubmed/31963439
http://dx.doi.org/10.3390/mi11010100
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