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Characterization and Analysis of the Skin Microbiota in Acne: Impact of Systemic Antibiotics

Systemic antibiotics are extensively used to control moderate to severe acne. Hence, it is crucial to understand their impact on the skin microbiota, which is supposedly perturbed. The purpose of this study was to compare the makeup and diversity of the skin microbiota in acne patients before and af...

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Autores principales: Park, Seo-Yeon, Kim, Hei Sung, Lee, Se Hoon, Kim, Sungjoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019264/
https://www.ncbi.nlm.nih.gov/pubmed/31936262
http://dx.doi.org/10.3390/jcm9010168
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author Park, Seo-Yeon
Kim, Hei Sung
Lee, Se Hoon
Kim, Sungjoo
author_facet Park, Seo-Yeon
Kim, Hei Sung
Lee, Se Hoon
Kim, Sungjoo
author_sort Park, Seo-Yeon
collection PubMed
description Systemic antibiotics are extensively used to control moderate to severe acne. Hence, it is crucial to understand their impact on the skin microbiota, which is supposedly perturbed. The purpose of this study was to compare the makeup and diversity of the skin microbiota in acne patients before and after taking oral antibiotics. A longitudinal cohort study was performed on 20 participants with moderate to severe facial acne with no recent use of oral and topical antibiotics/retinoids. Patients were prescribed oral doxycycline, 100 mg, twice daily for six weeks. Skin areas on the cheek were sampled for 16S ribosomal RNA gene sequencing at baseline, and after six weeks of doxycycline treatment. Ten males and 10 females aged 11 to 44 years with a median Investigator’s Global Assessment score of 3 (moderate) were enrolled. At baseline, Cutibacterium acnes (formerly Propionibacterium acnes) was the most dominant species followed by Staphylococcus epidermidis. Acne severity showed a positive correlation with the abundance of Cutibacterium acnes. Across all subjects, antibiotic treatment reduced clinical acne grades and was associated with a 1.96-fold reduction in the relative abundance of Cutibacterium acnes (p = 0.01, 95% CI −22% to −3%). Marked changes were also identified in other bacterial species, such as Cutibacterium granulosum (formerly Propionibacterium granulosum), which increased by 4.46-fold (p = 0.02, 95% CI 0.004% to 0.9%) in the treated samples. In general, antibiotics administration was associated with an increase in bacterial diversity (alpha diversity). Principal coordinates analysis showed mild clustering of samples by patient (analysis of similarity, R = 0.135, p = 0.04) whereas there was scant clustering with treatment (ANOSIM, R = 0.005; p = 0.29). In conclusion, we found individuals with acne to have a unique microbial signature. Acne treatment with systemic antibiotics was associated with changes in the composition and diversity of skin microbiota, especially Cutibacterium acnes, which correlates with acne severity. Our study provides insight into the skin microbiota in acne and how it is modulated by systemic antibiotics.
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spelling pubmed-70192642020-03-04 Characterization and Analysis of the Skin Microbiota in Acne: Impact of Systemic Antibiotics Park, Seo-Yeon Kim, Hei Sung Lee, Se Hoon Kim, Sungjoo J Clin Med Article Systemic antibiotics are extensively used to control moderate to severe acne. Hence, it is crucial to understand their impact on the skin microbiota, which is supposedly perturbed. The purpose of this study was to compare the makeup and diversity of the skin microbiota in acne patients before and after taking oral antibiotics. A longitudinal cohort study was performed on 20 participants with moderate to severe facial acne with no recent use of oral and topical antibiotics/retinoids. Patients were prescribed oral doxycycline, 100 mg, twice daily for six weeks. Skin areas on the cheek were sampled for 16S ribosomal RNA gene sequencing at baseline, and after six weeks of doxycycline treatment. Ten males and 10 females aged 11 to 44 years with a median Investigator’s Global Assessment score of 3 (moderate) were enrolled. At baseline, Cutibacterium acnes (formerly Propionibacterium acnes) was the most dominant species followed by Staphylococcus epidermidis. Acne severity showed a positive correlation with the abundance of Cutibacterium acnes. Across all subjects, antibiotic treatment reduced clinical acne grades and was associated with a 1.96-fold reduction in the relative abundance of Cutibacterium acnes (p = 0.01, 95% CI −22% to −3%). Marked changes were also identified in other bacterial species, such as Cutibacterium granulosum (formerly Propionibacterium granulosum), which increased by 4.46-fold (p = 0.02, 95% CI 0.004% to 0.9%) in the treated samples. In general, antibiotics administration was associated with an increase in bacterial diversity (alpha diversity). Principal coordinates analysis showed mild clustering of samples by patient (analysis of similarity, R = 0.135, p = 0.04) whereas there was scant clustering with treatment (ANOSIM, R = 0.005; p = 0.29). In conclusion, we found individuals with acne to have a unique microbial signature. Acne treatment with systemic antibiotics was associated with changes in the composition and diversity of skin microbiota, especially Cutibacterium acnes, which correlates with acne severity. Our study provides insight into the skin microbiota in acne and how it is modulated by systemic antibiotics. MDPI 2020-01-08 /pmc/articles/PMC7019264/ /pubmed/31936262 http://dx.doi.org/10.3390/jcm9010168 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Seo-Yeon
Kim, Hei Sung
Lee, Se Hoon
Kim, Sungjoo
Characterization and Analysis of the Skin Microbiota in Acne: Impact of Systemic Antibiotics
title Characterization and Analysis of the Skin Microbiota in Acne: Impact of Systemic Antibiotics
title_full Characterization and Analysis of the Skin Microbiota in Acne: Impact of Systemic Antibiotics
title_fullStr Characterization and Analysis of the Skin Microbiota in Acne: Impact of Systemic Antibiotics
title_full_unstemmed Characterization and Analysis of the Skin Microbiota in Acne: Impact of Systemic Antibiotics
title_short Characterization and Analysis of the Skin Microbiota in Acne: Impact of Systemic Antibiotics
title_sort characterization and analysis of the skin microbiota in acne: impact of systemic antibiotics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019264/
https://www.ncbi.nlm.nih.gov/pubmed/31936262
http://dx.doi.org/10.3390/jcm9010168
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