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DEF Cell-Derived Exosomal miR-148a-5p Promotes DTMUV Replication by Negative Regulating TLR3 Expression
Duck tembusu virus (DTMUV) is a single-stranded, positive-polarity RNA flavivirus that has caused considerable economic losses in China in recent years. Innate immunity represents the first line of defense against invading pathogens and serves as an important role in resisting viral infections. In t...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019286/ https://www.ncbi.nlm.nih.gov/pubmed/31947624 http://dx.doi.org/10.3390/v12010094 |
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author | Guo, Hongyan Cheng, Anchun Zhang, Xingcui Pan, YuHong Wang, Mingshu Huang, Juan Zhu, Dekang Chen, Shun Liu, Mafeng Zhao, Xinxin Wu, Ying Yang, Qiao Zhang, Shaqiu Yu, Yanling Pan, Leichang Tian, Bin Rehman, Mujeeb Ur Chen, Xiaoyue Liu, Yunya Zhang, Ling Yin, Zhongqiong Jing, Bo Jia, Renyong |
author_facet | Guo, Hongyan Cheng, Anchun Zhang, Xingcui Pan, YuHong Wang, Mingshu Huang, Juan Zhu, Dekang Chen, Shun Liu, Mafeng Zhao, Xinxin Wu, Ying Yang, Qiao Zhang, Shaqiu Yu, Yanling Pan, Leichang Tian, Bin Rehman, Mujeeb Ur Chen, Xiaoyue Liu, Yunya Zhang, Ling Yin, Zhongqiong Jing, Bo Jia, Renyong |
author_sort | Guo, Hongyan |
collection | PubMed |
description | Duck tembusu virus (DTMUV) is a single-stranded, positive-polarity RNA flavivirus that has caused considerable economic losses in China in recent years. Innate immunity represents the first line of defense against invading pathogens and serves as an important role in resisting viral infections. In this study, we found that the infection of ducks by DTMUV triggers Toll-like receptors (TLRs) and (RIG-I)-like receptors (RLRs) signaling pathways and inducing abundant of pro-inflammatory factors and type I interferons (IFNs), in which melanoma differentiation-associated gene 5 (MDA5) and Toll-like receptor 3 (TLR3) play important immunity roles, they can inhibit the replication process of DTMUV via inducing type I IFNs. Moreover, we demonstrated that type I IFNs can inhibit the DTMUV replication process in a time- and dose-dependent manner. Exosomes are small membrane vesicles that have important roles in intercellular communication. MicroRNAs (miRNAs) are small non-coding RNAs that can modulate gene expression and are common substances in exosomes. In our experiment, we successfully isolated DEF cells derived exosome for the first time and explored its function. Firstly, we found the expression of miR-148a-5p is significantly decreased following DTMUV infect. Then we found miR-148a-5p can target TLR3 and down-regulate the expression of TLR3, serving as a negative factor in innate immunity. Unfortunately, we cannot find miRNAs with different expression changes that can target MDA5. Lastly, our experimental results showed that TLR3 was one of the causes of miR-148a-5p reduction, suggesting that the high level of TLR3 after DTMUV infect can both trigger innate immunity and suppress miR-148a-5p to resist DTMUV. |
format | Online Article Text |
id | pubmed-7019286 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70192862020-03-04 DEF Cell-Derived Exosomal miR-148a-5p Promotes DTMUV Replication by Negative Regulating TLR3 Expression Guo, Hongyan Cheng, Anchun Zhang, Xingcui Pan, YuHong Wang, Mingshu Huang, Juan Zhu, Dekang Chen, Shun Liu, Mafeng Zhao, Xinxin Wu, Ying Yang, Qiao Zhang, Shaqiu Yu, Yanling Pan, Leichang Tian, Bin Rehman, Mujeeb Ur Chen, Xiaoyue Liu, Yunya Zhang, Ling Yin, Zhongqiong Jing, Bo Jia, Renyong Viruses Article Duck tembusu virus (DTMUV) is a single-stranded, positive-polarity RNA flavivirus that has caused considerable economic losses in China in recent years. Innate immunity represents the first line of defense against invading pathogens and serves as an important role in resisting viral infections. In this study, we found that the infection of ducks by DTMUV triggers Toll-like receptors (TLRs) and (RIG-I)-like receptors (RLRs) signaling pathways and inducing abundant of pro-inflammatory factors and type I interferons (IFNs), in which melanoma differentiation-associated gene 5 (MDA5) and Toll-like receptor 3 (TLR3) play important immunity roles, they can inhibit the replication process of DTMUV via inducing type I IFNs. Moreover, we demonstrated that type I IFNs can inhibit the DTMUV replication process in a time- and dose-dependent manner. Exosomes are small membrane vesicles that have important roles in intercellular communication. MicroRNAs (miRNAs) are small non-coding RNAs that can modulate gene expression and are common substances in exosomes. In our experiment, we successfully isolated DEF cells derived exosome for the first time and explored its function. Firstly, we found the expression of miR-148a-5p is significantly decreased following DTMUV infect. Then we found miR-148a-5p can target TLR3 and down-regulate the expression of TLR3, serving as a negative factor in innate immunity. Unfortunately, we cannot find miRNAs with different expression changes that can target MDA5. Lastly, our experimental results showed that TLR3 was one of the causes of miR-148a-5p reduction, suggesting that the high level of TLR3 after DTMUV infect can both trigger innate immunity and suppress miR-148a-5p to resist DTMUV. MDPI 2020-01-14 /pmc/articles/PMC7019286/ /pubmed/31947624 http://dx.doi.org/10.3390/v12010094 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Guo, Hongyan Cheng, Anchun Zhang, Xingcui Pan, YuHong Wang, Mingshu Huang, Juan Zhu, Dekang Chen, Shun Liu, Mafeng Zhao, Xinxin Wu, Ying Yang, Qiao Zhang, Shaqiu Yu, Yanling Pan, Leichang Tian, Bin Rehman, Mujeeb Ur Chen, Xiaoyue Liu, Yunya Zhang, Ling Yin, Zhongqiong Jing, Bo Jia, Renyong DEF Cell-Derived Exosomal miR-148a-5p Promotes DTMUV Replication by Negative Regulating TLR3 Expression |
title | DEF Cell-Derived Exosomal miR-148a-5p Promotes DTMUV Replication by Negative Regulating TLR3 Expression |
title_full | DEF Cell-Derived Exosomal miR-148a-5p Promotes DTMUV Replication by Negative Regulating TLR3 Expression |
title_fullStr | DEF Cell-Derived Exosomal miR-148a-5p Promotes DTMUV Replication by Negative Regulating TLR3 Expression |
title_full_unstemmed | DEF Cell-Derived Exosomal miR-148a-5p Promotes DTMUV Replication by Negative Regulating TLR3 Expression |
title_short | DEF Cell-Derived Exosomal miR-148a-5p Promotes DTMUV Replication by Negative Regulating TLR3 Expression |
title_sort | def cell-derived exosomal mir-148a-5p promotes dtmuv replication by negative regulating tlr3 expression |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019286/ https://www.ncbi.nlm.nih.gov/pubmed/31947624 http://dx.doi.org/10.3390/v12010094 |
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