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Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils
Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are the two most important pathogens of hand, foot, and mouth disease (HFMD). However, the neuropathogenesis of EV71 and CVA16 has not been elucidated. In our previous study, we established gerbils as a useful model for both EV71 and CVA16 infecti...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019476/ https://www.ncbi.nlm.nih.gov/pubmed/31906004 http://dx.doi.org/10.3390/v12010046 |
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author | Sun, Yi-Sheng Yang, Zhang-Nv Xu, Fang Chen, Chen Lu, Hang-Jing Jiang, Jian-Min Zhang, Yan-Jun Zhu, Han-Ping Yao, Ping-Ping |
author_facet | Sun, Yi-Sheng Yang, Zhang-Nv Xu, Fang Chen, Chen Lu, Hang-Jing Jiang, Jian-Min Zhang, Yan-Jun Zhu, Han-Ping Yao, Ping-Ping |
author_sort | Sun, Yi-Sheng |
collection | PubMed |
description | Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are the two most important pathogens of hand, foot, and mouth disease (HFMD). However, the neuropathogenesis of EV71 and CVA16 has not been elucidated. In our previous study, we established gerbils as a useful model for both EV71 and CVA16 infection. In this work, we used RNA-seq technology to analyze the global gene expression of the brainstem of EV71- and CVA16-infected gerbils. We found that 3434 genes were upregulated while 916 genes were downregulated in EV71-infected gerbils. In CVA16-infected gerbils, 1039 genes were upregulated, and 299 genes were downregulated. We also found significant dysregulation of cytokines, such as IP-10 and CXCL9, in the brainstem of gerbils. The expression levels of 10 of the most upregulated genes were confirmed by real-time RT-PCR, and the upregulated tendency of most genes was in accordance with the differential gene expression (DGE) results. Our work provided global gene expression analysis of virus-infected gerbils and laid a solid foundation for elucidating the neuropathogenesis mechanisms of EV71 and CVA16. |
format | Online Article Text |
id | pubmed-7019476 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70194762020-03-09 Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils Sun, Yi-Sheng Yang, Zhang-Nv Xu, Fang Chen, Chen Lu, Hang-Jing Jiang, Jian-Min Zhang, Yan-Jun Zhu, Han-Ping Yao, Ping-Ping Viruses Article Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are the two most important pathogens of hand, foot, and mouth disease (HFMD). However, the neuropathogenesis of EV71 and CVA16 has not been elucidated. In our previous study, we established gerbils as a useful model for both EV71 and CVA16 infection. In this work, we used RNA-seq technology to analyze the global gene expression of the brainstem of EV71- and CVA16-infected gerbils. We found that 3434 genes were upregulated while 916 genes were downregulated in EV71-infected gerbils. In CVA16-infected gerbils, 1039 genes were upregulated, and 299 genes were downregulated. We also found significant dysregulation of cytokines, such as IP-10 and CXCL9, in the brainstem of gerbils. The expression levels of 10 of the most upregulated genes were confirmed by real-time RT-PCR, and the upregulated tendency of most genes was in accordance with the differential gene expression (DGE) results. Our work provided global gene expression analysis of virus-infected gerbils and laid a solid foundation for elucidating the neuropathogenesis mechanisms of EV71 and CVA16. MDPI 2019-12-30 /pmc/articles/PMC7019476/ /pubmed/31906004 http://dx.doi.org/10.3390/v12010046 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sun, Yi-Sheng Yang, Zhang-Nv Xu, Fang Chen, Chen Lu, Hang-Jing Jiang, Jian-Min Zhang, Yan-Jun Zhu, Han-Ping Yao, Ping-Ping Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils |
title | Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils |
title_full | Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils |
title_fullStr | Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils |
title_full_unstemmed | Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils |
title_short | Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils |
title_sort | global gene expression analysis of the brainstem in ev71- and cva16-infected gerbils |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019476/ https://www.ncbi.nlm.nih.gov/pubmed/31906004 http://dx.doi.org/10.3390/v12010046 |
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