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Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils

Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are the two most important pathogens of hand, foot, and mouth disease (HFMD). However, the neuropathogenesis of EV71 and CVA16 has not been elucidated. In our previous study, we established gerbils as a useful model for both EV71 and CVA16 infecti...

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Autores principales: Sun, Yi-Sheng, Yang, Zhang-Nv, Xu, Fang, Chen, Chen, Lu, Hang-Jing, Jiang, Jian-Min, Zhang, Yan-Jun, Zhu, Han-Ping, Yao, Ping-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019476/
https://www.ncbi.nlm.nih.gov/pubmed/31906004
http://dx.doi.org/10.3390/v12010046
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author Sun, Yi-Sheng
Yang, Zhang-Nv
Xu, Fang
Chen, Chen
Lu, Hang-Jing
Jiang, Jian-Min
Zhang, Yan-Jun
Zhu, Han-Ping
Yao, Ping-Ping
author_facet Sun, Yi-Sheng
Yang, Zhang-Nv
Xu, Fang
Chen, Chen
Lu, Hang-Jing
Jiang, Jian-Min
Zhang, Yan-Jun
Zhu, Han-Ping
Yao, Ping-Ping
author_sort Sun, Yi-Sheng
collection PubMed
description Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are the two most important pathogens of hand, foot, and mouth disease (HFMD). However, the neuropathogenesis of EV71 and CVA16 has not been elucidated. In our previous study, we established gerbils as a useful model for both EV71 and CVA16 infection. In this work, we used RNA-seq technology to analyze the global gene expression of the brainstem of EV71- and CVA16-infected gerbils. We found that 3434 genes were upregulated while 916 genes were downregulated in EV71-infected gerbils. In CVA16-infected gerbils, 1039 genes were upregulated, and 299 genes were downregulated. We also found significant dysregulation of cytokines, such as IP-10 and CXCL9, in the brainstem of gerbils. The expression levels of 10 of the most upregulated genes were confirmed by real-time RT-PCR, and the upregulated tendency of most genes was in accordance with the differential gene expression (DGE) results. Our work provided global gene expression analysis of virus-infected gerbils and laid a solid foundation for elucidating the neuropathogenesis mechanisms of EV71 and CVA16.
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spelling pubmed-70194762020-03-09 Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils Sun, Yi-Sheng Yang, Zhang-Nv Xu, Fang Chen, Chen Lu, Hang-Jing Jiang, Jian-Min Zhang, Yan-Jun Zhu, Han-Ping Yao, Ping-Ping Viruses Article Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are the two most important pathogens of hand, foot, and mouth disease (HFMD). However, the neuropathogenesis of EV71 and CVA16 has not been elucidated. In our previous study, we established gerbils as a useful model for both EV71 and CVA16 infection. In this work, we used RNA-seq technology to analyze the global gene expression of the brainstem of EV71- and CVA16-infected gerbils. We found that 3434 genes were upregulated while 916 genes were downregulated in EV71-infected gerbils. In CVA16-infected gerbils, 1039 genes were upregulated, and 299 genes were downregulated. We also found significant dysregulation of cytokines, such as IP-10 and CXCL9, in the brainstem of gerbils. The expression levels of 10 of the most upregulated genes were confirmed by real-time RT-PCR, and the upregulated tendency of most genes was in accordance with the differential gene expression (DGE) results. Our work provided global gene expression analysis of virus-infected gerbils and laid a solid foundation for elucidating the neuropathogenesis mechanisms of EV71 and CVA16. MDPI 2019-12-30 /pmc/articles/PMC7019476/ /pubmed/31906004 http://dx.doi.org/10.3390/v12010046 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sun, Yi-Sheng
Yang, Zhang-Nv
Xu, Fang
Chen, Chen
Lu, Hang-Jing
Jiang, Jian-Min
Zhang, Yan-Jun
Zhu, Han-Ping
Yao, Ping-Ping
Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils
title Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils
title_full Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils
title_fullStr Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils
title_full_unstemmed Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils
title_short Global Gene Expression Analysis of the Brainstem in EV71- and CVA16-Infected Gerbils
title_sort global gene expression analysis of the brainstem in ev71- and cva16-infected gerbils
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019476/
https://www.ncbi.nlm.nih.gov/pubmed/31906004
http://dx.doi.org/10.3390/v12010046
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