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Infection with Foamy Virus in Wild Ruminants—Evidence for a New Virus Reservoir?

Foamy viruses (FVs) are widely distributed and infect many animal species including non-human primates, horses, cattle, and cats. Several reports also suggest that other species can be FV hosts. Since most of such studies involved livestock or companion animals, we aimed to test blood samples from w...

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Autores principales: Materniak-Kornas, Magdalena, Löchelt, Martin, Rola, Jerzy, Kuźmak, Jacek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019589/
https://www.ncbi.nlm.nih.gov/pubmed/31947727
http://dx.doi.org/10.3390/v12010058
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author Materniak-Kornas, Magdalena
Löchelt, Martin
Rola, Jerzy
Kuźmak, Jacek
author_facet Materniak-Kornas, Magdalena
Löchelt, Martin
Rola, Jerzy
Kuźmak, Jacek
author_sort Materniak-Kornas, Magdalena
collection PubMed
description Foamy viruses (FVs) are widely distributed and infect many animal species including non-human primates, horses, cattle, and cats. Several reports also suggest that other species can be FV hosts. Since most of such studies involved livestock or companion animals, we aimed to test blood samples from wild ruminants for the presence of FV-specific antibodies and, subsequently, genetic material. Out of 269 serum samples tested by ELISA with the bovine foamy virus (BFV) Gag and Bet antigens, 23 sera showed increased reactivity to at least one of them. High reactive sera represented 30% of bison samples and 7.5% of deer specimens. Eleven of the ELISA-positives were also strongly positive in immunoblot analyses. The peripheral blood DNA of seroreactive animals was tested by semi-nested PCR. The specific 275 bp fragment of the pol gene was amplified only in one sample collected from a red deer and the analysis of its sequence showed the highest homology for European BFV isolates. Such results may suggest the existence of a new FV reservoir in bison as well as in deer populations. Whether the origin of such infections stems from a new FV or is the result of BFV inter-species transmission remains to be clarified.
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spelling pubmed-70195892020-03-09 Infection with Foamy Virus in Wild Ruminants—Evidence for a New Virus Reservoir? Materniak-Kornas, Magdalena Löchelt, Martin Rola, Jerzy Kuźmak, Jacek Viruses Article Foamy viruses (FVs) are widely distributed and infect many animal species including non-human primates, horses, cattle, and cats. Several reports also suggest that other species can be FV hosts. Since most of such studies involved livestock or companion animals, we aimed to test blood samples from wild ruminants for the presence of FV-specific antibodies and, subsequently, genetic material. Out of 269 serum samples tested by ELISA with the bovine foamy virus (BFV) Gag and Bet antigens, 23 sera showed increased reactivity to at least one of them. High reactive sera represented 30% of bison samples and 7.5% of deer specimens. Eleven of the ELISA-positives were also strongly positive in immunoblot analyses. The peripheral blood DNA of seroreactive animals was tested by semi-nested PCR. The specific 275 bp fragment of the pol gene was amplified only in one sample collected from a red deer and the analysis of its sequence showed the highest homology for European BFV isolates. Such results may suggest the existence of a new FV reservoir in bison as well as in deer populations. Whether the origin of such infections stems from a new FV or is the result of BFV inter-species transmission remains to be clarified. MDPI 2020-01-03 /pmc/articles/PMC7019589/ /pubmed/31947727 http://dx.doi.org/10.3390/v12010058 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Materniak-Kornas, Magdalena
Löchelt, Martin
Rola, Jerzy
Kuźmak, Jacek
Infection with Foamy Virus in Wild Ruminants—Evidence for a New Virus Reservoir?
title Infection with Foamy Virus in Wild Ruminants—Evidence for a New Virus Reservoir?
title_full Infection with Foamy Virus in Wild Ruminants—Evidence for a New Virus Reservoir?
title_fullStr Infection with Foamy Virus in Wild Ruminants—Evidence for a New Virus Reservoir?
title_full_unstemmed Infection with Foamy Virus in Wild Ruminants—Evidence for a New Virus Reservoir?
title_short Infection with Foamy Virus in Wild Ruminants—Evidence for a New Virus Reservoir?
title_sort infection with foamy virus in wild ruminants—evidence for a new virus reservoir?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019589/
https://www.ncbi.nlm.nih.gov/pubmed/31947727
http://dx.doi.org/10.3390/v12010058
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