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Infection with Foamy Virus in Wild Ruminants—Evidence for a New Virus Reservoir?
Foamy viruses (FVs) are widely distributed and infect many animal species including non-human primates, horses, cattle, and cats. Several reports also suggest that other species can be FV hosts. Since most of such studies involved livestock or companion animals, we aimed to test blood samples from w...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019589/ https://www.ncbi.nlm.nih.gov/pubmed/31947727 http://dx.doi.org/10.3390/v12010058 |
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author | Materniak-Kornas, Magdalena Löchelt, Martin Rola, Jerzy Kuźmak, Jacek |
author_facet | Materniak-Kornas, Magdalena Löchelt, Martin Rola, Jerzy Kuźmak, Jacek |
author_sort | Materniak-Kornas, Magdalena |
collection | PubMed |
description | Foamy viruses (FVs) are widely distributed and infect many animal species including non-human primates, horses, cattle, and cats. Several reports also suggest that other species can be FV hosts. Since most of such studies involved livestock or companion animals, we aimed to test blood samples from wild ruminants for the presence of FV-specific antibodies and, subsequently, genetic material. Out of 269 serum samples tested by ELISA with the bovine foamy virus (BFV) Gag and Bet antigens, 23 sera showed increased reactivity to at least one of them. High reactive sera represented 30% of bison samples and 7.5% of deer specimens. Eleven of the ELISA-positives were also strongly positive in immunoblot analyses. The peripheral blood DNA of seroreactive animals was tested by semi-nested PCR. The specific 275 bp fragment of the pol gene was amplified only in one sample collected from a red deer and the analysis of its sequence showed the highest homology for European BFV isolates. Such results may suggest the existence of a new FV reservoir in bison as well as in deer populations. Whether the origin of such infections stems from a new FV or is the result of BFV inter-species transmission remains to be clarified. |
format | Online Article Text |
id | pubmed-7019589 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70195892020-03-09 Infection with Foamy Virus in Wild Ruminants—Evidence for a New Virus Reservoir? Materniak-Kornas, Magdalena Löchelt, Martin Rola, Jerzy Kuźmak, Jacek Viruses Article Foamy viruses (FVs) are widely distributed and infect many animal species including non-human primates, horses, cattle, and cats. Several reports also suggest that other species can be FV hosts. Since most of such studies involved livestock or companion animals, we aimed to test blood samples from wild ruminants for the presence of FV-specific antibodies and, subsequently, genetic material. Out of 269 serum samples tested by ELISA with the bovine foamy virus (BFV) Gag and Bet antigens, 23 sera showed increased reactivity to at least one of them. High reactive sera represented 30% of bison samples and 7.5% of deer specimens. Eleven of the ELISA-positives were also strongly positive in immunoblot analyses. The peripheral blood DNA of seroreactive animals was tested by semi-nested PCR. The specific 275 bp fragment of the pol gene was amplified only in one sample collected from a red deer and the analysis of its sequence showed the highest homology for European BFV isolates. Such results may suggest the existence of a new FV reservoir in bison as well as in deer populations. Whether the origin of such infections stems from a new FV or is the result of BFV inter-species transmission remains to be clarified. MDPI 2020-01-03 /pmc/articles/PMC7019589/ /pubmed/31947727 http://dx.doi.org/10.3390/v12010058 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Materniak-Kornas, Magdalena Löchelt, Martin Rola, Jerzy Kuźmak, Jacek Infection with Foamy Virus in Wild Ruminants—Evidence for a New Virus Reservoir? |
title | Infection with Foamy Virus in Wild Ruminants—Evidence for a New Virus Reservoir? |
title_full | Infection with Foamy Virus in Wild Ruminants—Evidence for a New Virus Reservoir? |
title_fullStr | Infection with Foamy Virus in Wild Ruminants—Evidence for a New Virus Reservoir? |
title_full_unstemmed | Infection with Foamy Virus in Wild Ruminants—Evidence for a New Virus Reservoir? |
title_short | Infection with Foamy Virus in Wild Ruminants—Evidence for a New Virus Reservoir? |
title_sort | infection with foamy virus in wild ruminants—evidence for a new virus reservoir? |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019589/ https://www.ncbi.nlm.nih.gov/pubmed/31947727 http://dx.doi.org/10.3390/v12010058 |
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