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Discovery and Prevalence of Divergent RNA Viruses in European Field Voles and Rabbits

The advent of unbiased metagenomic virus discovery has revolutionized studies of virus biodiversity and evolution. Despite this, our knowledge of the virosphere, including in mammalian species, remains limited. We used unbiased metagenomic sequencing to identify RNA viruses in European field voles a...

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Autores principales: Tsoleridis, Theocharis, Chappell, Joseph G., Monchatre-Leroy, Elodie, Umhang, Gérald, Shi, Mang, Bennett, Malcolm, Tarlinton, Rachael E., McClure, C. Patrick, Holmes, Edward C., Ball, Jonathan K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019641/
https://www.ncbi.nlm.nih.gov/pubmed/31906044
http://dx.doi.org/10.3390/v12010047
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author Tsoleridis, Theocharis
Chappell, Joseph G.
Monchatre-Leroy, Elodie
Umhang, Gérald
Shi, Mang
Bennett, Malcolm
Tarlinton, Rachael E.
McClure, C. Patrick
Holmes, Edward C.
Ball, Jonathan K.
author_facet Tsoleridis, Theocharis
Chappell, Joseph G.
Monchatre-Leroy, Elodie
Umhang, Gérald
Shi, Mang
Bennett, Malcolm
Tarlinton, Rachael E.
McClure, C. Patrick
Holmes, Edward C.
Ball, Jonathan K.
author_sort Tsoleridis, Theocharis
collection PubMed
description The advent of unbiased metagenomic virus discovery has revolutionized studies of virus biodiversity and evolution. Despite this, our knowledge of the virosphere, including in mammalian species, remains limited. We used unbiased metagenomic sequencing to identify RNA viruses in European field voles and rabbits. Accordingly, we identified a number of novel RNA viruses including astrovirus, rotavirus A, picorna-like virus and a narmovirus (paramyxovirus). In addition, we identified a sobemovirus and a novel luteovirus that likely originated from the rabbit diet. These newly discovered viruses were often divergent from those previously described. The novel astrovirus was most closely related to a virus sampled from the rodent-eating European roller bird (Coracias garrulous). PCR screening revealed that the novel narmovirus in the UK field vole had a prevalence of approximately 4%, and shared common ancestry with other rodent narmoviruses sampled globally. Two novel rotavirus A sequences were detected in a UK field vole and a French rabbit, the latter with a prevalence of 5%. Finally, a highly divergent picorna-like virus found in the gut of the French rabbit virus was only ~35% similar to an arilivirus at the amino acid level, suggesting the presence of a novel viral genus within the Picornaviridae.
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spelling pubmed-70196412020-03-09 Discovery and Prevalence of Divergent RNA Viruses in European Field Voles and Rabbits Tsoleridis, Theocharis Chappell, Joseph G. Monchatre-Leroy, Elodie Umhang, Gérald Shi, Mang Bennett, Malcolm Tarlinton, Rachael E. McClure, C. Patrick Holmes, Edward C. Ball, Jonathan K. Viruses Article The advent of unbiased metagenomic virus discovery has revolutionized studies of virus biodiversity and evolution. Despite this, our knowledge of the virosphere, including in mammalian species, remains limited. We used unbiased metagenomic sequencing to identify RNA viruses in European field voles and rabbits. Accordingly, we identified a number of novel RNA viruses including astrovirus, rotavirus A, picorna-like virus and a narmovirus (paramyxovirus). In addition, we identified a sobemovirus and a novel luteovirus that likely originated from the rabbit diet. These newly discovered viruses were often divergent from those previously described. The novel astrovirus was most closely related to a virus sampled from the rodent-eating European roller bird (Coracias garrulous). PCR screening revealed that the novel narmovirus in the UK field vole had a prevalence of approximately 4%, and shared common ancestry with other rodent narmoviruses sampled globally. Two novel rotavirus A sequences were detected in a UK field vole and a French rabbit, the latter with a prevalence of 5%. Finally, a highly divergent picorna-like virus found in the gut of the French rabbit virus was only ~35% similar to an arilivirus at the amino acid level, suggesting the presence of a novel viral genus within the Picornaviridae. MDPI 2019-12-31 /pmc/articles/PMC7019641/ /pubmed/31906044 http://dx.doi.org/10.3390/v12010047 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tsoleridis, Theocharis
Chappell, Joseph G.
Monchatre-Leroy, Elodie
Umhang, Gérald
Shi, Mang
Bennett, Malcolm
Tarlinton, Rachael E.
McClure, C. Patrick
Holmes, Edward C.
Ball, Jonathan K.
Discovery and Prevalence of Divergent RNA Viruses in European Field Voles and Rabbits
title Discovery and Prevalence of Divergent RNA Viruses in European Field Voles and Rabbits
title_full Discovery and Prevalence of Divergent RNA Viruses in European Field Voles and Rabbits
title_fullStr Discovery and Prevalence of Divergent RNA Viruses in European Field Voles and Rabbits
title_full_unstemmed Discovery and Prevalence of Divergent RNA Viruses in European Field Voles and Rabbits
title_short Discovery and Prevalence of Divergent RNA Viruses in European Field Voles and Rabbits
title_sort discovery and prevalence of divergent rna viruses in european field voles and rabbits
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019641/
https://www.ncbi.nlm.nih.gov/pubmed/31906044
http://dx.doi.org/10.3390/v12010047
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