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Discovery and Prevalence of Divergent RNA Viruses in European Field Voles and Rabbits
The advent of unbiased metagenomic virus discovery has revolutionized studies of virus biodiversity and evolution. Despite this, our knowledge of the virosphere, including in mammalian species, remains limited. We used unbiased metagenomic sequencing to identify RNA viruses in European field voles a...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019641/ https://www.ncbi.nlm.nih.gov/pubmed/31906044 http://dx.doi.org/10.3390/v12010047 |
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author | Tsoleridis, Theocharis Chappell, Joseph G. Monchatre-Leroy, Elodie Umhang, Gérald Shi, Mang Bennett, Malcolm Tarlinton, Rachael E. McClure, C. Patrick Holmes, Edward C. Ball, Jonathan K. |
author_facet | Tsoleridis, Theocharis Chappell, Joseph G. Monchatre-Leroy, Elodie Umhang, Gérald Shi, Mang Bennett, Malcolm Tarlinton, Rachael E. McClure, C. Patrick Holmes, Edward C. Ball, Jonathan K. |
author_sort | Tsoleridis, Theocharis |
collection | PubMed |
description | The advent of unbiased metagenomic virus discovery has revolutionized studies of virus biodiversity and evolution. Despite this, our knowledge of the virosphere, including in mammalian species, remains limited. We used unbiased metagenomic sequencing to identify RNA viruses in European field voles and rabbits. Accordingly, we identified a number of novel RNA viruses including astrovirus, rotavirus A, picorna-like virus and a narmovirus (paramyxovirus). In addition, we identified a sobemovirus and a novel luteovirus that likely originated from the rabbit diet. These newly discovered viruses were often divergent from those previously described. The novel astrovirus was most closely related to a virus sampled from the rodent-eating European roller bird (Coracias garrulous). PCR screening revealed that the novel narmovirus in the UK field vole had a prevalence of approximately 4%, and shared common ancestry with other rodent narmoviruses sampled globally. Two novel rotavirus A sequences were detected in a UK field vole and a French rabbit, the latter with a prevalence of 5%. Finally, a highly divergent picorna-like virus found in the gut of the French rabbit virus was only ~35% similar to an arilivirus at the amino acid level, suggesting the presence of a novel viral genus within the Picornaviridae. |
format | Online Article Text |
id | pubmed-7019641 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70196412020-03-09 Discovery and Prevalence of Divergent RNA Viruses in European Field Voles and Rabbits Tsoleridis, Theocharis Chappell, Joseph G. Monchatre-Leroy, Elodie Umhang, Gérald Shi, Mang Bennett, Malcolm Tarlinton, Rachael E. McClure, C. Patrick Holmes, Edward C. Ball, Jonathan K. Viruses Article The advent of unbiased metagenomic virus discovery has revolutionized studies of virus biodiversity and evolution. Despite this, our knowledge of the virosphere, including in mammalian species, remains limited. We used unbiased metagenomic sequencing to identify RNA viruses in European field voles and rabbits. Accordingly, we identified a number of novel RNA viruses including astrovirus, rotavirus A, picorna-like virus and a narmovirus (paramyxovirus). In addition, we identified a sobemovirus and a novel luteovirus that likely originated from the rabbit diet. These newly discovered viruses were often divergent from those previously described. The novel astrovirus was most closely related to a virus sampled from the rodent-eating European roller bird (Coracias garrulous). PCR screening revealed that the novel narmovirus in the UK field vole had a prevalence of approximately 4%, and shared common ancestry with other rodent narmoviruses sampled globally. Two novel rotavirus A sequences were detected in a UK field vole and a French rabbit, the latter with a prevalence of 5%. Finally, a highly divergent picorna-like virus found in the gut of the French rabbit virus was only ~35% similar to an arilivirus at the amino acid level, suggesting the presence of a novel viral genus within the Picornaviridae. MDPI 2019-12-31 /pmc/articles/PMC7019641/ /pubmed/31906044 http://dx.doi.org/10.3390/v12010047 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tsoleridis, Theocharis Chappell, Joseph G. Monchatre-Leroy, Elodie Umhang, Gérald Shi, Mang Bennett, Malcolm Tarlinton, Rachael E. McClure, C. Patrick Holmes, Edward C. Ball, Jonathan K. Discovery and Prevalence of Divergent RNA Viruses in European Field Voles and Rabbits |
title | Discovery and Prevalence of Divergent RNA Viruses in European Field Voles and Rabbits |
title_full | Discovery and Prevalence of Divergent RNA Viruses in European Field Voles and Rabbits |
title_fullStr | Discovery and Prevalence of Divergent RNA Viruses in European Field Voles and Rabbits |
title_full_unstemmed | Discovery and Prevalence of Divergent RNA Viruses in European Field Voles and Rabbits |
title_short | Discovery and Prevalence of Divergent RNA Viruses in European Field Voles and Rabbits |
title_sort | discovery and prevalence of divergent rna viruses in european field voles and rabbits |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019641/ https://www.ncbi.nlm.nih.gov/pubmed/31906044 http://dx.doi.org/10.3390/v12010047 |
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