Cargando…

The Systemic Redox Status Is Maintained in Non-Smoking Type 2 Diabetic Subjects Without Cardiovascular Disease: Association with Elevated Triglycerides and Large VLDL

Decreased circulating levels of free thiols (R-SH, sulfhydryl groups) reflect enhanced oxidative stress, which plays an important role in the pathogenesis of cardiometabolic diseases. Since hyperglycemia causes oxidative stress, we questioned whether plasma free thiols are altered in patients with t...

Descripción completa

Detalles Bibliográficos
Autores principales: van Dijk, Peter R., Abdulle, Amaal Eman, Bulthuis, Marian L.C., Perton, Frank G., Connelly, Margery A., van Goor, Harry, Dullaart, Robin P.F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019670/
https://www.ncbi.nlm.nih.gov/pubmed/31878321
http://dx.doi.org/10.3390/jcm9010049
_version_ 1783497573006311424
author van Dijk, Peter R.
Abdulle, Amaal Eman
Bulthuis, Marian L.C.
Perton, Frank G.
Connelly, Margery A.
van Goor, Harry
Dullaart, Robin P.F.
author_facet van Dijk, Peter R.
Abdulle, Amaal Eman
Bulthuis, Marian L.C.
Perton, Frank G.
Connelly, Margery A.
van Goor, Harry
Dullaart, Robin P.F.
author_sort van Dijk, Peter R.
collection PubMed
description Decreased circulating levels of free thiols (R-SH, sulfhydryl groups) reflect enhanced oxidative stress, which plays an important role in the pathogenesis of cardiometabolic diseases. Since hyperglycemia causes oxidative stress, we questioned whether plasma free thiols are altered in patients with type 2 diabetes mellitus (T2DM) without cardiovascular disease or renal function impairment. We also determined their relationship with elevated triglycerides and very low density lipoproteins (VLDL), a central feature of diabetic dyslipidemia. Fasting plasma free thiols (colorimetric method), lipoproteins, VLDL (nuclear magnetic resonance spectrometry), free fatty acids (FFA), phospholipid transfer protein (PLTP) activity and adiponectin were measured in 79 adult non-smoking T2DM subjects (HbA1c 51 ± 8 mmol/mol, no use of insulin or lipid lowering drugs), and in 89 non-smoking subjects without T2DM. Plasma free thiols were univariately correlated with glucose (r = 0.196, p < 0.05), but were not decreased in T2DM subjects versus non-diabetic subjects (p = 0.31). Free thiols were higher in subjects with (663 ± 84 µmol/L) versus subjects without elevated triglycerides (619 ± 91 µmol/L; p = 0.002). Age- and sex-adjusted multivariable linear regression analysis demonstrated that plasma triglycerides were positively and independently associated with free thiols (β = 0.215, p = 0.004), FFA (β = 0.168, p = 0.029) and PLTP activity (β = 0.228, p = 0.002), inversely with adiponectin (β = −0.308, p < 0.001) but not with glucose (β = 0.052, p = 0.51). Notably, the positive association of free thiols with (elevated) triglycerides appeared to be particularly evident in men. Additionally, large VLDL were independently associated with free thiols (β = 0.188, p = 0.029). In conclusion, circulating free thiols are not decreased in this cohort of non-smoking and generally well-controlled T2DM subjects. Paradoxically, higher triglycerides and more large VLDL particles are likely associated with higher plasma levels of thiols, reflecting lower systemic oxidative stress.
format Online
Article
Text
id pubmed-7019670
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70196702020-03-09 The Systemic Redox Status Is Maintained in Non-Smoking Type 2 Diabetic Subjects Without Cardiovascular Disease: Association with Elevated Triglycerides and Large VLDL van Dijk, Peter R. Abdulle, Amaal Eman Bulthuis, Marian L.C. Perton, Frank G. Connelly, Margery A. van Goor, Harry Dullaart, Robin P.F. J Clin Med Article Decreased circulating levels of free thiols (R-SH, sulfhydryl groups) reflect enhanced oxidative stress, which plays an important role in the pathogenesis of cardiometabolic diseases. Since hyperglycemia causes oxidative stress, we questioned whether plasma free thiols are altered in patients with type 2 diabetes mellitus (T2DM) without cardiovascular disease or renal function impairment. We also determined their relationship with elevated triglycerides and very low density lipoproteins (VLDL), a central feature of diabetic dyslipidemia. Fasting plasma free thiols (colorimetric method), lipoproteins, VLDL (nuclear magnetic resonance spectrometry), free fatty acids (FFA), phospholipid transfer protein (PLTP) activity and adiponectin were measured in 79 adult non-smoking T2DM subjects (HbA1c 51 ± 8 mmol/mol, no use of insulin or lipid lowering drugs), and in 89 non-smoking subjects without T2DM. Plasma free thiols were univariately correlated with glucose (r = 0.196, p < 0.05), but were not decreased in T2DM subjects versus non-diabetic subjects (p = 0.31). Free thiols were higher in subjects with (663 ± 84 µmol/L) versus subjects without elevated triglycerides (619 ± 91 µmol/L; p = 0.002). Age- and sex-adjusted multivariable linear regression analysis demonstrated that plasma triglycerides were positively and independently associated with free thiols (β = 0.215, p = 0.004), FFA (β = 0.168, p = 0.029) and PLTP activity (β = 0.228, p = 0.002), inversely with adiponectin (β = −0.308, p < 0.001) but not with glucose (β = 0.052, p = 0.51). Notably, the positive association of free thiols with (elevated) triglycerides appeared to be particularly evident in men. Additionally, large VLDL were independently associated with free thiols (β = 0.188, p = 0.029). In conclusion, circulating free thiols are not decreased in this cohort of non-smoking and generally well-controlled T2DM subjects. Paradoxically, higher triglycerides and more large VLDL particles are likely associated with higher plasma levels of thiols, reflecting lower systemic oxidative stress. MDPI 2019-12-24 /pmc/articles/PMC7019670/ /pubmed/31878321 http://dx.doi.org/10.3390/jcm9010049 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
van Dijk, Peter R.
Abdulle, Amaal Eman
Bulthuis, Marian L.C.
Perton, Frank G.
Connelly, Margery A.
van Goor, Harry
Dullaart, Robin P.F.
The Systemic Redox Status Is Maintained in Non-Smoking Type 2 Diabetic Subjects Without Cardiovascular Disease: Association with Elevated Triglycerides and Large VLDL
title The Systemic Redox Status Is Maintained in Non-Smoking Type 2 Diabetic Subjects Without Cardiovascular Disease: Association with Elevated Triglycerides and Large VLDL
title_full The Systemic Redox Status Is Maintained in Non-Smoking Type 2 Diabetic Subjects Without Cardiovascular Disease: Association with Elevated Triglycerides and Large VLDL
title_fullStr The Systemic Redox Status Is Maintained in Non-Smoking Type 2 Diabetic Subjects Without Cardiovascular Disease: Association with Elevated Triglycerides and Large VLDL
title_full_unstemmed The Systemic Redox Status Is Maintained in Non-Smoking Type 2 Diabetic Subjects Without Cardiovascular Disease: Association with Elevated Triglycerides and Large VLDL
title_short The Systemic Redox Status Is Maintained in Non-Smoking Type 2 Diabetic Subjects Without Cardiovascular Disease: Association with Elevated Triglycerides and Large VLDL
title_sort systemic redox status is maintained in non-smoking type 2 diabetic subjects without cardiovascular disease: association with elevated triglycerides and large vldl
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019670/
https://www.ncbi.nlm.nih.gov/pubmed/31878321
http://dx.doi.org/10.3390/jcm9010049
work_keys_str_mv AT vandijkpeterr thesystemicredoxstatusismaintainedinnonsmokingtype2diabeticsubjectswithoutcardiovasculardiseaseassociationwithelevatedtriglyceridesandlargevldl
AT abdulleamaaleman thesystemicredoxstatusismaintainedinnonsmokingtype2diabeticsubjectswithoutcardiovasculardiseaseassociationwithelevatedtriglyceridesandlargevldl
AT bulthuismarianlc thesystemicredoxstatusismaintainedinnonsmokingtype2diabeticsubjectswithoutcardiovasculardiseaseassociationwithelevatedtriglyceridesandlargevldl
AT pertonfrankg thesystemicredoxstatusismaintainedinnonsmokingtype2diabeticsubjectswithoutcardiovasculardiseaseassociationwithelevatedtriglyceridesandlargevldl
AT connellymargerya thesystemicredoxstatusismaintainedinnonsmokingtype2diabeticsubjectswithoutcardiovasculardiseaseassociationwithelevatedtriglyceridesandlargevldl
AT vangoorharry thesystemicredoxstatusismaintainedinnonsmokingtype2diabeticsubjectswithoutcardiovasculardiseaseassociationwithelevatedtriglyceridesandlargevldl
AT dullaartrobinpf thesystemicredoxstatusismaintainedinnonsmokingtype2diabeticsubjectswithoutcardiovasculardiseaseassociationwithelevatedtriglyceridesandlargevldl
AT vandijkpeterr systemicredoxstatusismaintainedinnonsmokingtype2diabeticsubjectswithoutcardiovasculardiseaseassociationwithelevatedtriglyceridesandlargevldl
AT abdulleamaaleman systemicredoxstatusismaintainedinnonsmokingtype2diabeticsubjectswithoutcardiovasculardiseaseassociationwithelevatedtriglyceridesandlargevldl
AT bulthuismarianlc systemicredoxstatusismaintainedinnonsmokingtype2diabeticsubjectswithoutcardiovasculardiseaseassociationwithelevatedtriglyceridesandlargevldl
AT pertonfrankg systemicredoxstatusismaintainedinnonsmokingtype2diabeticsubjectswithoutcardiovasculardiseaseassociationwithelevatedtriglyceridesandlargevldl
AT connellymargerya systemicredoxstatusismaintainedinnonsmokingtype2diabeticsubjectswithoutcardiovasculardiseaseassociationwithelevatedtriglyceridesandlargevldl
AT vangoorharry systemicredoxstatusismaintainedinnonsmokingtype2diabeticsubjectswithoutcardiovasculardiseaseassociationwithelevatedtriglyceridesandlargevldl
AT dullaartrobinpf systemicredoxstatusismaintainedinnonsmokingtype2diabeticsubjectswithoutcardiovasculardiseaseassociationwithelevatedtriglyceridesandlargevldl