Cargando…

Cellular Oxidative Stress in Pediatric Leukemia and Lymphoma Patients Undergoing Treatment Is Associated with Protein Consumption

Over and under nutrition are associated with worse outcomes for children with leukemia and lymphoma; however, the molecular basis for this clinical observation is not well understood. Many chemotherapeutics used for leukemia treatment are known to generate oxidative stress in vitro; therefore, we ev...

Descripción completa

Detalles Bibliográficos
Autores principales: Raber, Margaret, Wu, Jimin, Donnella, Hayley, Knouse, Phillip, Pise, Mayurika, Munsell, Mark, Liu, Diane, Chandra, Joya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019785/
https://www.ncbi.nlm.nih.gov/pubmed/31892127
http://dx.doi.org/10.3390/nu12010075
_version_ 1783497599738707968
author Raber, Margaret
Wu, Jimin
Donnella, Hayley
Knouse, Phillip
Pise, Mayurika
Munsell, Mark
Liu, Diane
Chandra, Joya
author_facet Raber, Margaret
Wu, Jimin
Donnella, Hayley
Knouse, Phillip
Pise, Mayurika
Munsell, Mark
Liu, Diane
Chandra, Joya
author_sort Raber, Margaret
collection PubMed
description Over and under nutrition are associated with worse outcomes for children with leukemia and lymphoma; however, the molecular basis for this clinical observation is not well understood. Many chemotherapeutics used for leukemia treatment are known to generate oxidative stress in vitro; therefore, we evaluated redox status and diet in pediatric leukemia patients during therapy in order to ascertain relationships between nutrition and oxidative stress. Dietary intake and redox measures in peripheral blood mononuclear cells from 32 pediatric leukemia and lymphoma patients were collected over six months during treatment. Baseline measures when patients were off chemotherapy and subsequent assessments were collected after one, two and six months. Oxidative stress increased over time in all patients, consistent with chemotherapy-induced redox effects. Older and younger children showed significantly different baseline levels of reactive oxygen species, which increased over time in all age ranges. Diet was assessed at points proximal to oxidative stress measurements and revealed a novel association with consumption of animal protein, vegetable protein, and total protein intake. Our findings demonstrate that chemotherapy increases oxidative stress in pediatric leukemia patients, and raises the possibility that dietary protein or altered protein metabolism could contribute to clinical outcomes.
format Online
Article
Text
id pubmed-7019785
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70197852020-03-09 Cellular Oxidative Stress in Pediatric Leukemia and Lymphoma Patients Undergoing Treatment Is Associated with Protein Consumption Raber, Margaret Wu, Jimin Donnella, Hayley Knouse, Phillip Pise, Mayurika Munsell, Mark Liu, Diane Chandra, Joya Nutrients Article Over and under nutrition are associated with worse outcomes for children with leukemia and lymphoma; however, the molecular basis for this clinical observation is not well understood. Many chemotherapeutics used for leukemia treatment are known to generate oxidative stress in vitro; therefore, we evaluated redox status and diet in pediatric leukemia patients during therapy in order to ascertain relationships between nutrition and oxidative stress. Dietary intake and redox measures in peripheral blood mononuclear cells from 32 pediatric leukemia and lymphoma patients were collected over six months during treatment. Baseline measures when patients were off chemotherapy and subsequent assessments were collected after one, two and six months. Oxidative stress increased over time in all patients, consistent with chemotherapy-induced redox effects. Older and younger children showed significantly different baseline levels of reactive oxygen species, which increased over time in all age ranges. Diet was assessed at points proximal to oxidative stress measurements and revealed a novel association with consumption of animal protein, vegetable protein, and total protein intake. Our findings demonstrate that chemotherapy increases oxidative stress in pediatric leukemia patients, and raises the possibility that dietary protein or altered protein metabolism could contribute to clinical outcomes. MDPI 2019-12-27 /pmc/articles/PMC7019785/ /pubmed/31892127 http://dx.doi.org/10.3390/nu12010075 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Raber, Margaret
Wu, Jimin
Donnella, Hayley
Knouse, Phillip
Pise, Mayurika
Munsell, Mark
Liu, Diane
Chandra, Joya
Cellular Oxidative Stress in Pediatric Leukemia and Lymphoma Patients Undergoing Treatment Is Associated with Protein Consumption
title Cellular Oxidative Stress in Pediatric Leukemia and Lymphoma Patients Undergoing Treatment Is Associated with Protein Consumption
title_full Cellular Oxidative Stress in Pediatric Leukemia and Lymphoma Patients Undergoing Treatment Is Associated with Protein Consumption
title_fullStr Cellular Oxidative Stress in Pediatric Leukemia and Lymphoma Patients Undergoing Treatment Is Associated with Protein Consumption
title_full_unstemmed Cellular Oxidative Stress in Pediatric Leukemia and Lymphoma Patients Undergoing Treatment Is Associated with Protein Consumption
title_short Cellular Oxidative Stress in Pediatric Leukemia and Lymphoma Patients Undergoing Treatment Is Associated with Protein Consumption
title_sort cellular oxidative stress in pediatric leukemia and lymphoma patients undergoing treatment is associated with protein consumption
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019785/
https://www.ncbi.nlm.nih.gov/pubmed/31892127
http://dx.doi.org/10.3390/nu12010075
work_keys_str_mv AT rabermargaret cellularoxidativestressinpediatricleukemiaandlymphomapatientsundergoingtreatmentisassociatedwithproteinconsumption
AT wujimin cellularoxidativestressinpediatricleukemiaandlymphomapatientsundergoingtreatmentisassociatedwithproteinconsumption
AT donnellahayley cellularoxidativestressinpediatricleukemiaandlymphomapatientsundergoingtreatmentisassociatedwithproteinconsumption
AT knousephillip cellularoxidativestressinpediatricleukemiaandlymphomapatientsundergoingtreatmentisassociatedwithproteinconsumption
AT pisemayurika cellularoxidativestressinpediatricleukemiaandlymphomapatientsundergoingtreatmentisassociatedwithproteinconsumption
AT munsellmark cellularoxidativestressinpediatricleukemiaandlymphomapatientsundergoingtreatmentisassociatedwithproteinconsumption
AT liudiane cellularoxidativestressinpediatricleukemiaandlymphomapatientsundergoingtreatmentisassociatedwithproteinconsumption
AT chandrajoya cellularoxidativestressinpediatricleukemiaandlymphomapatientsundergoingtreatmentisassociatedwithproteinconsumption