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Presence of ROS in Inflammatory Environment of Peri-Implantitis Tissue: In Vitro and In Vivo Human Evidence

Analyses of composition, distribution of cellular and extracellular matrix components, and molecular analysis of mitochondria related genes of bone loss in the presence of inflammatory environment in humans was the aim of the present project. As a human model we chose peri-implantitis. Morphological...

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Autores principales: Mijiritsky, Eitan, Ferroni, Letizia, Gardin, Chiara, Peleg, Oren, Gultekin, Alper, Saglanmak, Alper, Delogu, Lucia Gemma, Mitrecic, Dinko, Piattelli, Adriano, Tatullo, Marco, Zavan, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019824/
https://www.ncbi.nlm.nih.gov/pubmed/31878038
http://dx.doi.org/10.3390/jcm9010038
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author Mijiritsky, Eitan
Ferroni, Letizia
Gardin, Chiara
Peleg, Oren
Gultekin, Alper
Saglanmak, Alper
Delogu, Lucia Gemma
Mitrecic, Dinko
Piattelli, Adriano
Tatullo, Marco
Zavan, Barbara
author_facet Mijiritsky, Eitan
Ferroni, Letizia
Gardin, Chiara
Peleg, Oren
Gultekin, Alper
Saglanmak, Alper
Delogu, Lucia Gemma
Mitrecic, Dinko
Piattelli, Adriano
Tatullo, Marco
Zavan, Barbara
author_sort Mijiritsky, Eitan
collection PubMed
description Analyses of composition, distribution of cellular and extracellular matrix components, and molecular analysis of mitochondria related genes of bone loss in the presence of inflammatory environment in humans was the aim of the present project. As a human model we chose peri-implantitis. Morphological analyses were performed by means classical histological, immunohistochemical, and SEM (scanning electron miscroscopy) test. Gene expression analysis was performed to evaluate epithelium maturation, collagen fiber production, and genes related to mitochondrial activity. It was found that a well-defined keratinocyte epithelium was present on the top of all specimens; a distinct basal lamina was present, as well as desmosomes and autophagic processes related to the maturation of keratinocytes. Under this epithelium, a full inflammatory cell infiltrate was present for about 60% of the represented by plasma cells. Collagen type I fibers were present mainly in the form of fragmented cord tissue without cells. A different distribution of blood vessels was also present from the apical to the most coronal portion of the specimens. High levels of genes related to oxidative stress were present, as well as the activation of genes related to the loss of ability of osteogenic commitment of Mesenchymal stem cells into osteoblasts. Our study suggests that peri-implantitis lesions exhibit a well defined biological organization not only in terms of inflammatory cells but also on vessel and extracellular matrix components even if no difference in the epithelium is evident, and that the presence of reactive oxygen species (ROS) related to the inflammatory environment influences the correct commitment of Mesenchymal stem cells.
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spelling pubmed-70198242020-03-09 Presence of ROS in Inflammatory Environment of Peri-Implantitis Tissue: In Vitro and In Vivo Human Evidence Mijiritsky, Eitan Ferroni, Letizia Gardin, Chiara Peleg, Oren Gultekin, Alper Saglanmak, Alper Delogu, Lucia Gemma Mitrecic, Dinko Piattelli, Adriano Tatullo, Marco Zavan, Barbara J Clin Med Article Analyses of composition, distribution of cellular and extracellular matrix components, and molecular analysis of mitochondria related genes of bone loss in the presence of inflammatory environment in humans was the aim of the present project. As a human model we chose peri-implantitis. Morphological analyses were performed by means classical histological, immunohistochemical, and SEM (scanning electron miscroscopy) test. Gene expression analysis was performed to evaluate epithelium maturation, collagen fiber production, and genes related to mitochondrial activity. It was found that a well-defined keratinocyte epithelium was present on the top of all specimens; a distinct basal lamina was present, as well as desmosomes and autophagic processes related to the maturation of keratinocytes. Under this epithelium, a full inflammatory cell infiltrate was present for about 60% of the represented by plasma cells. Collagen type I fibers were present mainly in the form of fragmented cord tissue without cells. A different distribution of blood vessels was also present from the apical to the most coronal portion of the specimens. High levels of genes related to oxidative stress were present, as well as the activation of genes related to the loss of ability of osteogenic commitment of Mesenchymal stem cells into osteoblasts. Our study suggests that peri-implantitis lesions exhibit a well defined biological organization not only in terms of inflammatory cells but also on vessel and extracellular matrix components even if no difference in the epithelium is evident, and that the presence of reactive oxygen species (ROS) related to the inflammatory environment influences the correct commitment of Mesenchymal stem cells. MDPI 2019-12-23 /pmc/articles/PMC7019824/ /pubmed/31878038 http://dx.doi.org/10.3390/jcm9010038 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mijiritsky, Eitan
Ferroni, Letizia
Gardin, Chiara
Peleg, Oren
Gultekin, Alper
Saglanmak, Alper
Delogu, Lucia Gemma
Mitrecic, Dinko
Piattelli, Adriano
Tatullo, Marco
Zavan, Barbara
Presence of ROS in Inflammatory Environment of Peri-Implantitis Tissue: In Vitro and In Vivo Human Evidence
title Presence of ROS in Inflammatory Environment of Peri-Implantitis Tissue: In Vitro and In Vivo Human Evidence
title_full Presence of ROS in Inflammatory Environment of Peri-Implantitis Tissue: In Vitro and In Vivo Human Evidence
title_fullStr Presence of ROS in Inflammatory Environment of Peri-Implantitis Tissue: In Vitro and In Vivo Human Evidence
title_full_unstemmed Presence of ROS in Inflammatory Environment of Peri-Implantitis Tissue: In Vitro and In Vivo Human Evidence
title_short Presence of ROS in Inflammatory Environment of Peri-Implantitis Tissue: In Vitro and In Vivo Human Evidence
title_sort presence of ros in inflammatory environment of peri-implantitis tissue: in vitro and in vivo human evidence
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019824/
https://www.ncbi.nlm.nih.gov/pubmed/31878038
http://dx.doi.org/10.3390/jcm9010038
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