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Split-Liver Ex Situ Machine Perfusion: A Novel Technique for Studying Organ Preservation and Therapeutic Interventions
Ex situ machine perfusion is a promising technology to help improve organ viability prior to transplantation. However, preclinical studies using discarded human livers to evaluate therapeutic interventions and optimize perfusion conditions are limited by significant graft heterogeneity. In order to...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019984/ https://www.ncbi.nlm.nih.gov/pubmed/31963739 http://dx.doi.org/10.3390/jcm9010269 |
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author | Huang, Viola Karimian, Negin Detelich, Danielle Raigani, Siavash Geerts, Sharon Beijert, Irene Fontan, Fermin M. Aburawi, Mohamed M. Ozer, Sinan Banik, Peony Lin, Florence Karabacak, Murat Hafiz, Ehab O.A. Porte, Robert J. Uygun, Korkut Markmann, James F. Yeh, Heidi |
author_facet | Huang, Viola Karimian, Negin Detelich, Danielle Raigani, Siavash Geerts, Sharon Beijert, Irene Fontan, Fermin M. Aburawi, Mohamed M. Ozer, Sinan Banik, Peony Lin, Florence Karabacak, Murat Hafiz, Ehab O.A. Porte, Robert J. Uygun, Korkut Markmann, James F. Yeh, Heidi |
author_sort | Huang, Viola |
collection | PubMed |
description | Ex situ machine perfusion is a promising technology to help improve organ viability prior to transplantation. However, preclinical studies using discarded human livers to evaluate therapeutic interventions and optimize perfusion conditions are limited by significant graft heterogeneity. In order to improve the efficacy and reproducibility of future studies, a split-liver perfusion model was developed to allow simultaneous perfusion of left and right lobes, allowing one lobe to serve as a control for the other. Eleven discarded livers were surgically split, and both lobes perfused simultaneously on separate perfusion devices for 3 h at subnormothermic temperatures. Lobar perfusion parameters were also compared with whole livers undergoing perfusion. Similar to whole-liver perfusions, each lobe in the split-liver model exhibited a progressive decrease in arterial resistance and lactate levels throughout perfusion, which were not significantly different between right and left lobes. Split liver lobes also demonstrated comparable energy charge ratios. Ex situ split-liver perfusion is a novel experimental model that allows each graft to act as its own control. This model is particularly well suited for preclinical studies by avoiding the need for large numbers of enrolled livers necessary due to the heterogenous nature of discarded human liver research. |
format | Online Article Text |
id | pubmed-7019984 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70199842020-03-09 Split-Liver Ex Situ Machine Perfusion: A Novel Technique for Studying Organ Preservation and Therapeutic Interventions Huang, Viola Karimian, Negin Detelich, Danielle Raigani, Siavash Geerts, Sharon Beijert, Irene Fontan, Fermin M. Aburawi, Mohamed M. Ozer, Sinan Banik, Peony Lin, Florence Karabacak, Murat Hafiz, Ehab O.A. Porte, Robert J. Uygun, Korkut Markmann, James F. Yeh, Heidi J Clin Med Article Ex situ machine perfusion is a promising technology to help improve organ viability prior to transplantation. However, preclinical studies using discarded human livers to evaluate therapeutic interventions and optimize perfusion conditions are limited by significant graft heterogeneity. In order to improve the efficacy and reproducibility of future studies, a split-liver perfusion model was developed to allow simultaneous perfusion of left and right lobes, allowing one lobe to serve as a control for the other. Eleven discarded livers were surgically split, and both lobes perfused simultaneously on separate perfusion devices for 3 h at subnormothermic temperatures. Lobar perfusion parameters were also compared with whole livers undergoing perfusion. Similar to whole-liver perfusions, each lobe in the split-liver model exhibited a progressive decrease in arterial resistance and lactate levels throughout perfusion, which were not significantly different between right and left lobes. Split liver lobes also demonstrated comparable energy charge ratios. Ex situ split-liver perfusion is a novel experimental model that allows each graft to act as its own control. This model is particularly well suited for preclinical studies by avoiding the need for large numbers of enrolled livers necessary due to the heterogenous nature of discarded human liver research. MDPI 2020-01-18 /pmc/articles/PMC7019984/ /pubmed/31963739 http://dx.doi.org/10.3390/jcm9010269 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Huang, Viola Karimian, Negin Detelich, Danielle Raigani, Siavash Geerts, Sharon Beijert, Irene Fontan, Fermin M. Aburawi, Mohamed M. Ozer, Sinan Banik, Peony Lin, Florence Karabacak, Murat Hafiz, Ehab O.A. Porte, Robert J. Uygun, Korkut Markmann, James F. Yeh, Heidi Split-Liver Ex Situ Machine Perfusion: A Novel Technique for Studying Organ Preservation and Therapeutic Interventions |
title | Split-Liver Ex Situ Machine Perfusion: A Novel Technique for Studying Organ Preservation and Therapeutic Interventions |
title_full | Split-Liver Ex Situ Machine Perfusion: A Novel Technique for Studying Organ Preservation and Therapeutic Interventions |
title_fullStr | Split-Liver Ex Situ Machine Perfusion: A Novel Technique for Studying Organ Preservation and Therapeutic Interventions |
title_full_unstemmed | Split-Liver Ex Situ Machine Perfusion: A Novel Technique for Studying Organ Preservation and Therapeutic Interventions |
title_short | Split-Liver Ex Situ Machine Perfusion: A Novel Technique for Studying Organ Preservation and Therapeutic Interventions |
title_sort | split-liver ex situ machine perfusion: a novel technique for studying organ preservation and therapeutic interventions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019984/ https://www.ncbi.nlm.nih.gov/pubmed/31963739 http://dx.doi.org/10.3390/jcm9010269 |
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