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Split-Liver Ex Situ Machine Perfusion: A Novel Technique for Studying Organ Preservation and Therapeutic Interventions

Ex situ machine perfusion is a promising technology to help improve organ viability prior to transplantation. However, preclinical studies using discarded human livers to evaluate therapeutic interventions and optimize perfusion conditions are limited by significant graft heterogeneity. In order to...

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Autores principales: Huang, Viola, Karimian, Negin, Detelich, Danielle, Raigani, Siavash, Geerts, Sharon, Beijert, Irene, Fontan, Fermin M., Aburawi, Mohamed M., Ozer, Sinan, Banik, Peony, Lin, Florence, Karabacak, Murat, Hafiz, Ehab O.A., Porte, Robert J., Uygun, Korkut, Markmann, James F., Yeh, Heidi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019984/
https://www.ncbi.nlm.nih.gov/pubmed/31963739
http://dx.doi.org/10.3390/jcm9010269
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author Huang, Viola
Karimian, Negin
Detelich, Danielle
Raigani, Siavash
Geerts, Sharon
Beijert, Irene
Fontan, Fermin M.
Aburawi, Mohamed M.
Ozer, Sinan
Banik, Peony
Lin, Florence
Karabacak, Murat
Hafiz, Ehab O.A.
Porte, Robert J.
Uygun, Korkut
Markmann, James F.
Yeh, Heidi
author_facet Huang, Viola
Karimian, Negin
Detelich, Danielle
Raigani, Siavash
Geerts, Sharon
Beijert, Irene
Fontan, Fermin M.
Aburawi, Mohamed M.
Ozer, Sinan
Banik, Peony
Lin, Florence
Karabacak, Murat
Hafiz, Ehab O.A.
Porte, Robert J.
Uygun, Korkut
Markmann, James F.
Yeh, Heidi
author_sort Huang, Viola
collection PubMed
description Ex situ machine perfusion is a promising technology to help improve organ viability prior to transplantation. However, preclinical studies using discarded human livers to evaluate therapeutic interventions and optimize perfusion conditions are limited by significant graft heterogeneity. In order to improve the efficacy and reproducibility of future studies, a split-liver perfusion model was developed to allow simultaneous perfusion of left and right lobes, allowing one lobe to serve as a control for the other. Eleven discarded livers were surgically split, and both lobes perfused simultaneously on separate perfusion devices for 3 h at subnormothermic temperatures. Lobar perfusion parameters were also compared with whole livers undergoing perfusion. Similar to whole-liver perfusions, each lobe in the split-liver model exhibited a progressive decrease in arterial resistance and lactate levels throughout perfusion, which were not significantly different between right and left lobes. Split liver lobes also demonstrated comparable energy charge ratios. Ex situ split-liver perfusion is a novel experimental model that allows each graft to act as its own control. This model is particularly well suited for preclinical studies by avoiding the need for large numbers of enrolled livers necessary due to the heterogenous nature of discarded human liver research.
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spelling pubmed-70199842020-03-09 Split-Liver Ex Situ Machine Perfusion: A Novel Technique for Studying Organ Preservation and Therapeutic Interventions Huang, Viola Karimian, Negin Detelich, Danielle Raigani, Siavash Geerts, Sharon Beijert, Irene Fontan, Fermin M. Aburawi, Mohamed M. Ozer, Sinan Banik, Peony Lin, Florence Karabacak, Murat Hafiz, Ehab O.A. Porte, Robert J. Uygun, Korkut Markmann, James F. Yeh, Heidi J Clin Med Article Ex situ machine perfusion is a promising technology to help improve organ viability prior to transplantation. However, preclinical studies using discarded human livers to evaluate therapeutic interventions and optimize perfusion conditions are limited by significant graft heterogeneity. In order to improve the efficacy and reproducibility of future studies, a split-liver perfusion model was developed to allow simultaneous perfusion of left and right lobes, allowing one lobe to serve as a control for the other. Eleven discarded livers were surgically split, and both lobes perfused simultaneously on separate perfusion devices for 3 h at subnormothermic temperatures. Lobar perfusion parameters were also compared with whole livers undergoing perfusion. Similar to whole-liver perfusions, each lobe in the split-liver model exhibited a progressive decrease in arterial resistance and lactate levels throughout perfusion, which were not significantly different between right and left lobes. Split liver lobes also demonstrated comparable energy charge ratios. Ex situ split-liver perfusion is a novel experimental model that allows each graft to act as its own control. This model is particularly well suited for preclinical studies by avoiding the need for large numbers of enrolled livers necessary due to the heterogenous nature of discarded human liver research. MDPI 2020-01-18 /pmc/articles/PMC7019984/ /pubmed/31963739 http://dx.doi.org/10.3390/jcm9010269 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Huang, Viola
Karimian, Negin
Detelich, Danielle
Raigani, Siavash
Geerts, Sharon
Beijert, Irene
Fontan, Fermin M.
Aburawi, Mohamed M.
Ozer, Sinan
Banik, Peony
Lin, Florence
Karabacak, Murat
Hafiz, Ehab O.A.
Porte, Robert J.
Uygun, Korkut
Markmann, James F.
Yeh, Heidi
Split-Liver Ex Situ Machine Perfusion: A Novel Technique for Studying Organ Preservation and Therapeutic Interventions
title Split-Liver Ex Situ Machine Perfusion: A Novel Technique for Studying Organ Preservation and Therapeutic Interventions
title_full Split-Liver Ex Situ Machine Perfusion: A Novel Technique for Studying Organ Preservation and Therapeutic Interventions
title_fullStr Split-Liver Ex Situ Machine Perfusion: A Novel Technique for Studying Organ Preservation and Therapeutic Interventions
title_full_unstemmed Split-Liver Ex Situ Machine Perfusion: A Novel Technique for Studying Organ Preservation and Therapeutic Interventions
title_short Split-Liver Ex Situ Machine Perfusion: A Novel Technique for Studying Organ Preservation and Therapeutic Interventions
title_sort split-liver ex situ machine perfusion: a novel technique for studying organ preservation and therapeutic interventions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019984/
https://www.ncbi.nlm.nih.gov/pubmed/31963739
http://dx.doi.org/10.3390/jcm9010269
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