Cargando…

A Modular, Reconfigurable Microfabricated Assembly Platform for Microfluidic Transport and Multitype Cell Culture and Drug Testing

Modular microfluidics offer the opportunity to combine the precise fluid control, rapid sample processing, low sample and reagent volumes, and relatively lower cost of conventional microfluidics with the flexible reconfigurability needed to accommodate the requirements of target applications such as...

Descripción completa

Detalles Bibliográficos
Autores principales: Xie, Xin, Maharjan, Sushila, Liu, Sanwei, Zhang, Yu Shrike, Livermore, Carol
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020019/
https://www.ncbi.nlm.nih.gov/pubmed/31861298
http://dx.doi.org/10.3390/mi11010002
_version_ 1783497654192308224
author Xie, Xin
Maharjan, Sushila
Liu, Sanwei
Zhang, Yu Shrike
Livermore, Carol
author_facet Xie, Xin
Maharjan, Sushila
Liu, Sanwei
Zhang, Yu Shrike
Livermore, Carol
author_sort Xie, Xin
collection PubMed
description Modular microfluidics offer the opportunity to combine the precise fluid control, rapid sample processing, low sample and reagent volumes, and relatively lower cost of conventional microfluidics with the flexible reconfigurability needed to accommodate the requirements of target applications such as drug toxicity studies. However, combining the capabilities of fully adaptable modular microelectromechanical systems (MEMS) assembly with the simplicity of conventional microfluidic fabrication remains a challenge. A hybrid polydimethylsiloxane (PDMS)-molding/photolithographic process is demonstrated to rapidly fabricate LEGO(®)-like modular blocks. The blocks are created with different sizes that interlock via tongue-and-groove joints in the plane and stack via interference fits out of the plane. These miniature strong but reversible connections have a measured resistance to in-plane and out-of-plane forces of up to >6000× and >1000× the weight of the block itself, respectively. The LEGO(®)-like interference fits enable O-ring-free microfluidic connections that withstand internal fluid pressures of >120 kPa. A single layer of blocks is assembled into LEGO(®)-like cell culture plates, where the in vitro biocompatibility and drug toxicity to lung epithelial adenocarcinoma cells and hepatocellular carcinoma cells cultured in the modular microwells are measured. A double-layer block structure is then assembled so that a microchannel formed at the interface between layers connects two microwells. Breast tumor cells and hepatocytes cultured in the coupled wells demonstrate interwell migration as well as the simultaneous effects of a single drug on the two cell types.
format Online
Article
Text
id pubmed-7020019
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70200192020-03-09 A Modular, Reconfigurable Microfabricated Assembly Platform for Microfluidic Transport and Multitype Cell Culture and Drug Testing Xie, Xin Maharjan, Sushila Liu, Sanwei Zhang, Yu Shrike Livermore, Carol Micromachines (Basel) Article Modular microfluidics offer the opportunity to combine the precise fluid control, rapid sample processing, low sample and reagent volumes, and relatively lower cost of conventional microfluidics with the flexible reconfigurability needed to accommodate the requirements of target applications such as drug toxicity studies. However, combining the capabilities of fully adaptable modular microelectromechanical systems (MEMS) assembly with the simplicity of conventional microfluidic fabrication remains a challenge. A hybrid polydimethylsiloxane (PDMS)-molding/photolithographic process is demonstrated to rapidly fabricate LEGO(®)-like modular blocks. The blocks are created with different sizes that interlock via tongue-and-groove joints in the plane and stack via interference fits out of the plane. These miniature strong but reversible connections have a measured resistance to in-plane and out-of-plane forces of up to >6000× and >1000× the weight of the block itself, respectively. The LEGO(®)-like interference fits enable O-ring-free microfluidic connections that withstand internal fluid pressures of >120 kPa. A single layer of blocks is assembled into LEGO(®)-like cell culture plates, where the in vitro biocompatibility and drug toxicity to lung epithelial adenocarcinoma cells and hepatocellular carcinoma cells cultured in the modular microwells are measured. A double-layer block structure is then assembled so that a microchannel formed at the interface between layers connects two microwells. Breast tumor cells and hepatocytes cultured in the coupled wells demonstrate interwell migration as well as the simultaneous effects of a single drug on the two cell types. MDPI 2019-12-18 /pmc/articles/PMC7020019/ /pubmed/31861298 http://dx.doi.org/10.3390/mi11010002 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xie, Xin
Maharjan, Sushila
Liu, Sanwei
Zhang, Yu Shrike
Livermore, Carol
A Modular, Reconfigurable Microfabricated Assembly Platform for Microfluidic Transport and Multitype Cell Culture and Drug Testing
title A Modular, Reconfigurable Microfabricated Assembly Platform for Microfluidic Transport and Multitype Cell Culture and Drug Testing
title_full A Modular, Reconfigurable Microfabricated Assembly Platform for Microfluidic Transport and Multitype Cell Culture and Drug Testing
title_fullStr A Modular, Reconfigurable Microfabricated Assembly Platform for Microfluidic Transport and Multitype Cell Culture and Drug Testing
title_full_unstemmed A Modular, Reconfigurable Microfabricated Assembly Platform for Microfluidic Transport and Multitype Cell Culture and Drug Testing
title_short A Modular, Reconfigurable Microfabricated Assembly Platform for Microfluidic Transport and Multitype Cell Culture and Drug Testing
title_sort modular, reconfigurable microfabricated assembly platform for microfluidic transport and multitype cell culture and drug testing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020019/
https://www.ncbi.nlm.nih.gov/pubmed/31861298
http://dx.doi.org/10.3390/mi11010002
work_keys_str_mv AT xiexin amodularreconfigurablemicrofabricatedassemblyplatformformicrofluidictransportandmultitypecellcultureanddrugtesting
AT maharjansushila amodularreconfigurablemicrofabricatedassemblyplatformformicrofluidictransportandmultitypecellcultureanddrugtesting
AT liusanwei amodularreconfigurablemicrofabricatedassemblyplatformformicrofluidictransportandmultitypecellcultureanddrugtesting
AT zhangyushrike amodularreconfigurablemicrofabricatedassemblyplatformformicrofluidictransportandmultitypecellcultureanddrugtesting
AT livermorecarol amodularreconfigurablemicrofabricatedassemblyplatformformicrofluidictransportandmultitypecellcultureanddrugtesting
AT xiexin modularreconfigurablemicrofabricatedassemblyplatformformicrofluidictransportandmultitypecellcultureanddrugtesting
AT maharjansushila modularreconfigurablemicrofabricatedassemblyplatformformicrofluidictransportandmultitypecellcultureanddrugtesting
AT liusanwei modularreconfigurablemicrofabricatedassemblyplatformformicrofluidictransportandmultitypecellcultureanddrugtesting
AT zhangyushrike modularreconfigurablemicrofabricatedassemblyplatformformicrofluidictransportandmultitypecellcultureanddrugtesting
AT livermorecarol modularreconfigurablemicrofabricatedassemblyplatformformicrofluidictransportandmultitypecellcultureanddrugtesting