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Expression Patterns of Circulating MicroRNAs in the Risk Stratification of Bicuspid Aortopathy

Objective: Aortic size-based criteria are of limited value in the prediction of aortic events, while most aortic events occur in patients with proximal aortic diameters < 50 mm. Serological biomarkers and especially circulating microRNAs (miRNAs) have been proposed as an elegant tool to improve r...

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Autores principales: Girdauskas, Evaldas, Neumann, Niklas, Petersen, Johannes, Sequeira-Gross, Tatiana, Naito, Shiho, von Stumm, Maria, von Kodolitsch, Yskert, Reichenspurner, Hermann, Zeller, Tanja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020030/
https://www.ncbi.nlm.nih.gov/pubmed/31963884
http://dx.doi.org/10.3390/jcm9010276
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author Girdauskas, Evaldas
Neumann, Niklas
Petersen, Johannes
Sequeira-Gross, Tatiana
Naito, Shiho
von Stumm, Maria
von Kodolitsch, Yskert
Reichenspurner, Hermann
Zeller, Tanja
author_facet Girdauskas, Evaldas
Neumann, Niklas
Petersen, Johannes
Sequeira-Gross, Tatiana
Naito, Shiho
von Stumm, Maria
von Kodolitsch, Yskert
Reichenspurner, Hermann
Zeller, Tanja
author_sort Girdauskas, Evaldas
collection PubMed
description Objective: Aortic size-based criteria are of limited value in the prediction of aortic events, while most aortic events occur in patients with proximal aortic diameters < 50 mm. Serological biomarkers and especially circulating microRNAs (miRNAs) have been proposed as an elegant tool to improve risk stratification in patients with different aortopathies. Therefore, we aimed to evaluate the levels of circulating miRNAs in a surgical cohort of patients presenting with bicuspid aortic valve disease and distinct valvulo-aortic phenotypes. Methods: We prospectively examined a consecutive cohort of 145 patients referred for aortic valve surgery: (1) Sixty three patients (mean age 47 ± 11 years, 92% male) with bicuspid aortic valve regurgitation and root dilatation (BAV-AR), (2) thirty two patients (mean age 59 ± 11 years, 73% male) with bicuspid aortic valve stenosis (BAV-AS), and (3) fifty patients (mean age 56 ± 14 years, 55% male) with tricuspid aortic valve stenosis and normal aortic root diameters (TAV-AS) who underwent aortic valve+/-proximal aortic surgery at a single institution. MicroRNAs analysis included 11 miRNAs, all published previously in association with aortopathies. Endpoints of our study were (1) correlation between circulating miRNAs and aortic diameter and (2) comparison of circulating miRNAs in distinct valvulo-aortic phenotypes. Results: We found a significant inverse linear correlation between circulating miRNAs levels and proximal aortic diameter in the whole study cohort. The strongest correlation was found for miR-17 (r = −0.42, p < 0.001), miR-20a (r = −0.37, p < 0.001), and miR-106a (r = −0.32, p < 0.001). All miRNAs were significantly downregulated in BAV vs. TAV with normal aortic root dimensions Conclusions: Our data demonstrate a significant inverse correlation between circulating miRNAs levels and the maximal aortic diameter in BAV aortopathy. When comparing miRNAs expression patterns in BAV vs. TAV patients with normal aortic root dimensions, BAV patients showed significant downregulation of analyzed miRNAs as compared to their TAV counterparts. Further multicenter studies in larger cohorts are needed to further validate these results.
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spelling pubmed-70200302020-03-09 Expression Patterns of Circulating MicroRNAs in the Risk Stratification of Bicuspid Aortopathy Girdauskas, Evaldas Neumann, Niklas Petersen, Johannes Sequeira-Gross, Tatiana Naito, Shiho von Stumm, Maria von Kodolitsch, Yskert Reichenspurner, Hermann Zeller, Tanja J Clin Med Article Objective: Aortic size-based criteria are of limited value in the prediction of aortic events, while most aortic events occur in patients with proximal aortic diameters < 50 mm. Serological biomarkers and especially circulating microRNAs (miRNAs) have been proposed as an elegant tool to improve risk stratification in patients with different aortopathies. Therefore, we aimed to evaluate the levels of circulating miRNAs in a surgical cohort of patients presenting with bicuspid aortic valve disease and distinct valvulo-aortic phenotypes. Methods: We prospectively examined a consecutive cohort of 145 patients referred for aortic valve surgery: (1) Sixty three patients (mean age 47 ± 11 years, 92% male) with bicuspid aortic valve regurgitation and root dilatation (BAV-AR), (2) thirty two patients (mean age 59 ± 11 years, 73% male) with bicuspid aortic valve stenosis (BAV-AS), and (3) fifty patients (mean age 56 ± 14 years, 55% male) with tricuspid aortic valve stenosis and normal aortic root diameters (TAV-AS) who underwent aortic valve+/-proximal aortic surgery at a single institution. MicroRNAs analysis included 11 miRNAs, all published previously in association with aortopathies. Endpoints of our study were (1) correlation between circulating miRNAs and aortic diameter and (2) comparison of circulating miRNAs in distinct valvulo-aortic phenotypes. Results: We found a significant inverse linear correlation between circulating miRNAs levels and proximal aortic diameter in the whole study cohort. The strongest correlation was found for miR-17 (r = −0.42, p < 0.001), miR-20a (r = −0.37, p < 0.001), and miR-106a (r = −0.32, p < 0.001). All miRNAs were significantly downregulated in BAV vs. TAV with normal aortic root dimensions Conclusions: Our data demonstrate a significant inverse correlation between circulating miRNAs levels and the maximal aortic diameter in BAV aortopathy. When comparing miRNAs expression patterns in BAV vs. TAV patients with normal aortic root dimensions, BAV patients showed significant downregulation of analyzed miRNAs as compared to their TAV counterparts. Further multicenter studies in larger cohorts are needed to further validate these results. MDPI 2020-01-19 /pmc/articles/PMC7020030/ /pubmed/31963884 http://dx.doi.org/10.3390/jcm9010276 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Girdauskas, Evaldas
Neumann, Niklas
Petersen, Johannes
Sequeira-Gross, Tatiana
Naito, Shiho
von Stumm, Maria
von Kodolitsch, Yskert
Reichenspurner, Hermann
Zeller, Tanja
Expression Patterns of Circulating MicroRNAs in the Risk Stratification of Bicuspid Aortopathy
title Expression Patterns of Circulating MicroRNAs in the Risk Stratification of Bicuspid Aortopathy
title_full Expression Patterns of Circulating MicroRNAs in the Risk Stratification of Bicuspid Aortopathy
title_fullStr Expression Patterns of Circulating MicroRNAs in the Risk Stratification of Bicuspid Aortopathy
title_full_unstemmed Expression Patterns of Circulating MicroRNAs in the Risk Stratification of Bicuspid Aortopathy
title_short Expression Patterns of Circulating MicroRNAs in the Risk Stratification of Bicuspid Aortopathy
title_sort expression patterns of circulating micrornas in the risk stratification of bicuspid aortopathy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020030/
https://www.ncbi.nlm.nih.gov/pubmed/31963884
http://dx.doi.org/10.3390/jcm9010276
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