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Morphological and adhesive properties of Klebsiella pneumoniae biofilms

BACKGROUND AND AIM: The study of biofilm-forming ability of Gram-negative microflora has great practical importance for assessing the effectiveness of antibiotic therapy and finding new ways to diagnose and inhibit the growth of biofilms. This is because poor penetration of antibacterial drugs into...

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Autores principales: Lenchenko, Ekaterina, Blumenkrants, Dmitry, Sachivkina, Nadezhda, Shadrova, Nadezhda, Ibragimova, Alfia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020135/
https://www.ncbi.nlm.nih.gov/pubmed/32158172
http://dx.doi.org/10.14202/vetworld.2020.197-200
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author Lenchenko, Ekaterina
Blumenkrants, Dmitry
Sachivkina, Nadezhda
Shadrova, Nadezhda
Ibragimova, Alfia
author_facet Lenchenko, Ekaterina
Blumenkrants, Dmitry
Sachivkina, Nadezhda
Shadrova, Nadezhda
Ibragimova, Alfia
author_sort Lenchenko, Ekaterina
collection PubMed
description BACKGROUND AND AIM: The study of biofilm-forming ability of Gram-negative microflora has great practical importance for assessing the effectiveness of antibiotic therapy and finding new ways to diagnose and inhibit the growth of biofilms. This is because poor penetration of antibacterial drugs into the biofilm can lead to the selection of resistant strains and has a consequence evident by the occurrence of relapse of infection in animals. This study aimed to evaluate morphological and densitometric indicators of biofilm formation as well as adhesive properties of Klebsiella pneumoniae. MATERIALS AND METHODS: K. pneumoniae was cultured at 37°C for 2-144 h in vitro. The specimens for optical microscopy were prepared by fixation with a 1:1 alcohol-ether mixture for 10 min and stained with a 0.5% solution of gentian violet for 2 min, and the optical density index was evaluated at a wavelength of 490 nm. Further, the adhesive properties of the microorganisms were determined at a concentration of 1 billion/ml and a suspension of ram erythrocytes at a concentration of 100 million/ml when cultured at 37°C for 24 h. Blood smears were prepared and stained with 0.5% gentian violet. RESULTS: K. pneumonia cultured at 37°C after 24 h on the meat peptone agar formed large, convex, mucous, and white colonies (d=3.0-6.0 mm). With the growth in the meat and peptone broth, uniform turbidity of the medium was observed. Analyzing the optical density indices (density, D), it was found that K. pneumoniae were good producers of biofilms (D=0.528±0.31). Data for indicators of adhesive properties of K. pneumoniae were as follows: Average adhesion index, 4.56±0.14; adhesion coefficient, 1.07±0.52; and adhesion index, 4.26±0.07. The studied bacteria had high adhesive activity. A direct correlation dependence (R=0.94) of the optical density of biofilms (D≥0.514-0.551) and AAI (4.15±0.28-4.76±0.75) was established. CONCLUSION: This study has demonstrated that K. pneumoniae had high adhesive activity, was strong producer of biofilms, and the optical density of the sample exceeded the optical density of the control by more than 4 times.
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spelling pubmed-70201352020-03-10 Morphological and adhesive properties of Klebsiella pneumoniae biofilms Lenchenko, Ekaterina Blumenkrants, Dmitry Sachivkina, Nadezhda Shadrova, Nadezhda Ibragimova, Alfia Vet World Research Article BACKGROUND AND AIM: The study of biofilm-forming ability of Gram-negative microflora has great practical importance for assessing the effectiveness of antibiotic therapy and finding new ways to diagnose and inhibit the growth of biofilms. This is because poor penetration of antibacterial drugs into the biofilm can lead to the selection of resistant strains and has a consequence evident by the occurrence of relapse of infection in animals. This study aimed to evaluate morphological and densitometric indicators of biofilm formation as well as adhesive properties of Klebsiella pneumoniae. MATERIALS AND METHODS: K. pneumoniae was cultured at 37°C for 2-144 h in vitro. The specimens for optical microscopy were prepared by fixation with a 1:1 alcohol-ether mixture for 10 min and stained with a 0.5% solution of gentian violet for 2 min, and the optical density index was evaluated at a wavelength of 490 nm. Further, the adhesive properties of the microorganisms were determined at a concentration of 1 billion/ml and a suspension of ram erythrocytes at a concentration of 100 million/ml when cultured at 37°C for 24 h. Blood smears were prepared and stained with 0.5% gentian violet. RESULTS: K. pneumonia cultured at 37°C after 24 h on the meat peptone agar formed large, convex, mucous, and white colonies (d=3.0-6.0 mm). With the growth in the meat and peptone broth, uniform turbidity of the medium was observed. Analyzing the optical density indices (density, D), it was found that K. pneumoniae were good producers of biofilms (D=0.528±0.31). Data for indicators of adhesive properties of K. pneumoniae were as follows: Average adhesion index, 4.56±0.14; adhesion coefficient, 1.07±0.52; and adhesion index, 4.26±0.07. The studied bacteria had high adhesive activity. A direct correlation dependence (R=0.94) of the optical density of biofilms (D≥0.514-0.551) and AAI (4.15±0.28-4.76±0.75) was established. CONCLUSION: This study has demonstrated that K. pneumoniae had high adhesive activity, was strong producer of biofilms, and the optical density of the sample exceeded the optical density of the control by more than 4 times. Veterinary World 2020-01 2020-01-28 /pmc/articles/PMC7020135/ /pubmed/32158172 http://dx.doi.org/10.14202/vetworld.2020.197-200 Text en Copyright: © Lenchenko, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Lenchenko, Ekaterina
Blumenkrants, Dmitry
Sachivkina, Nadezhda
Shadrova, Nadezhda
Ibragimova, Alfia
Morphological and adhesive properties of Klebsiella pneumoniae biofilms
title Morphological and adhesive properties of Klebsiella pneumoniae biofilms
title_full Morphological and adhesive properties of Klebsiella pneumoniae biofilms
title_fullStr Morphological and adhesive properties of Klebsiella pneumoniae biofilms
title_full_unstemmed Morphological and adhesive properties of Klebsiella pneumoniae biofilms
title_short Morphological and adhesive properties of Klebsiella pneumoniae biofilms
title_sort morphological and adhesive properties of klebsiella pneumoniae biofilms
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020135/
https://www.ncbi.nlm.nih.gov/pubmed/32158172
http://dx.doi.org/10.14202/vetworld.2020.197-200
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