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Comparison of the Androgenic Response of Spring and Winter Wheat (Triticum aestivum L.)

Androgenesis is potentially the most effective technique for doubled haploid production of wheat. It is not however widely used in breeding programmes due to its main limitation: the genotype dependence. Due to genetic differences between spring and winter wheat, it was assumed that both phenotypes...

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Autores principales: Weigt, Dorota, Kiel, Angelika, Siatkowski, Idzi, Zyprych-Walczak, Joanna, Tomkowiak, Agnieszka, Kwiatek, Michał
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020181/
https://www.ncbi.nlm.nih.gov/pubmed/31906148
http://dx.doi.org/10.3390/plants9010049
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author Weigt, Dorota
Kiel, Angelika
Siatkowski, Idzi
Zyprych-Walczak, Joanna
Tomkowiak, Agnieszka
Kwiatek, Michał
author_facet Weigt, Dorota
Kiel, Angelika
Siatkowski, Idzi
Zyprych-Walczak, Joanna
Tomkowiak, Agnieszka
Kwiatek, Michał
author_sort Weigt, Dorota
collection PubMed
description Androgenesis is potentially the most effective technique for doubled haploid production of wheat. It is not however widely used in breeding programmes due to its main limitation: the genotype dependence. Due to genetic differences between spring and winter wheat, it was assumed that both phenotypes are different in their capacity to conduct androgenesis. And so, the aim of this investigation was to verify the effectiveness of androgenesis induction and plant regeneration of spring and winter wheat genotypes while considering varying amounts of growth hormones in the induction medium. Fifteen genotypes of spring wheat and fifteen of winter wheat were used in the experiment. Six hundred anthers of each of the 30 genotypes were plated and analysed. Previous studies have allowed selection of the best medium for wheat androgenesis and a combination of growth hormones that are the most effective in stimulating microspore proliferation. Therefore, C17 induction media with two combinations of growth hormones were used: I—supplemented only by auxins (2,4-D and dicamba), and II—supplemented by auxin and cytokinin (2,4-D and kinetin). Data was recorded according to the efficiency of androgenic structure formation (ASF), green plant regeneration (GPR), and albino plant regeneration (APR). The results showed that the induction and regeneration of androgenesis in the spring wheat were more efficient than in the winter ones. The spring genotypes formed more androgenic structures and green plants on anthers plated on the medium supplemented only by auxins, in contrast to the winter genotypes which were better induced and regenerated on the medium supplemented by auxin and cytokinin. The study showed that to increase the efficiency of androgenesis, it is necessary to select appropriate factors such as concentration and type of hormones in medium composition, affecting the course of the culturing procedure according to the winter or spring phenotype of donor plants.
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spelling pubmed-70201812020-03-09 Comparison of the Androgenic Response of Spring and Winter Wheat (Triticum aestivum L.) Weigt, Dorota Kiel, Angelika Siatkowski, Idzi Zyprych-Walczak, Joanna Tomkowiak, Agnieszka Kwiatek, Michał Plants (Basel) Article Androgenesis is potentially the most effective technique for doubled haploid production of wheat. It is not however widely used in breeding programmes due to its main limitation: the genotype dependence. Due to genetic differences between spring and winter wheat, it was assumed that both phenotypes are different in their capacity to conduct androgenesis. And so, the aim of this investigation was to verify the effectiveness of androgenesis induction and plant regeneration of spring and winter wheat genotypes while considering varying amounts of growth hormones in the induction medium. Fifteen genotypes of spring wheat and fifteen of winter wheat were used in the experiment. Six hundred anthers of each of the 30 genotypes were plated and analysed. Previous studies have allowed selection of the best medium for wheat androgenesis and a combination of growth hormones that are the most effective in stimulating microspore proliferation. Therefore, C17 induction media with two combinations of growth hormones were used: I—supplemented only by auxins (2,4-D and dicamba), and II—supplemented by auxin and cytokinin (2,4-D and kinetin). Data was recorded according to the efficiency of androgenic structure formation (ASF), green plant regeneration (GPR), and albino plant regeneration (APR). The results showed that the induction and regeneration of androgenesis in the spring wheat were more efficient than in the winter ones. The spring genotypes formed more androgenic structures and green plants on anthers plated on the medium supplemented only by auxins, in contrast to the winter genotypes which were better induced and regenerated on the medium supplemented by auxin and cytokinin. The study showed that to increase the efficiency of androgenesis, it is necessary to select appropriate factors such as concentration and type of hormones in medium composition, affecting the course of the culturing procedure according to the winter or spring phenotype of donor plants. MDPI 2019-12-31 /pmc/articles/PMC7020181/ /pubmed/31906148 http://dx.doi.org/10.3390/plants9010049 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Weigt, Dorota
Kiel, Angelika
Siatkowski, Idzi
Zyprych-Walczak, Joanna
Tomkowiak, Agnieszka
Kwiatek, Michał
Comparison of the Androgenic Response of Spring and Winter Wheat (Triticum aestivum L.)
title Comparison of the Androgenic Response of Spring and Winter Wheat (Triticum aestivum L.)
title_full Comparison of the Androgenic Response of Spring and Winter Wheat (Triticum aestivum L.)
title_fullStr Comparison of the Androgenic Response of Spring and Winter Wheat (Triticum aestivum L.)
title_full_unstemmed Comparison of the Androgenic Response of Spring and Winter Wheat (Triticum aestivum L.)
title_short Comparison of the Androgenic Response of Spring and Winter Wheat (Triticum aestivum L.)
title_sort comparison of the androgenic response of spring and winter wheat (triticum aestivum l.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020181/
https://www.ncbi.nlm.nih.gov/pubmed/31906148
http://dx.doi.org/10.3390/plants9010049
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