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New EST-SSR Markers for Individual Genotyping of Opium Poppy Cultivars (Papaver somniferum L.)

High-quality simple sequence repeat (SSR) markers are invaluable tools for revealing genetic variability which could be utilized for many purposes, such as breeding new varieties or the identifying current ones, among other applications. Based on the analysis of 3.7 million EST sequences and 15 geno...

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Autores principales: Vašek, Jakub, Čílová, Daniela, Melounová, Martina, Svoboda, Pavel, Vejl, Pavel, Štikarová, Radka, Vostrý, Luboš, Kuchtová, Perla, Ovesná, Jaroslava
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020189/
https://www.ncbi.nlm.nih.gov/pubmed/31861643
http://dx.doi.org/10.3390/plants9010010
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author Vašek, Jakub
Čílová, Daniela
Melounová, Martina
Svoboda, Pavel
Vejl, Pavel
Štikarová, Radka
Vostrý, Luboš
Kuchtová, Perla
Ovesná, Jaroslava
author_facet Vašek, Jakub
Čílová, Daniela
Melounová, Martina
Svoboda, Pavel
Vejl, Pavel
Štikarová, Radka
Vostrý, Luboš
Kuchtová, Perla
Ovesná, Jaroslava
author_sort Vašek, Jakub
collection PubMed
description High-quality simple sequence repeat (SSR) markers are invaluable tools for revealing genetic variability which could be utilized for many purposes, such as breeding new varieties or the identifying current ones, among other applications. Based on the analysis of 3.7 million EST sequences and 15 genomic sequences from bacterial artificial chromosome (BAC) libraries, 200 trinucleotide genic (EST)-SSR and three genomic (gSSR) markers were tested, where 17 of them fulfilled all criteria for quality markers. Moreover, the reproducibility of these new markers was verified by two genetics laboratories, with a mean error rate per allele and per locus equal to 0.17%. These markers were tested on 38 accessions of Papaver somniferum and nine accessions of another five species of the Papaver and Argemone genera. In total, 118 alleles were detected for all accessions (median = 7; three to ten alleles per locus) and 88 alleles (median = 5; three to nine alleles per locus) within P. somniferum alone. Multivariate methods and identity analysis revealed high resolution capabilities of the new markers, where all but three pair accessions (41 out of 47) had a unique profile and opium poppy was distinguished from other species.
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spelling pubmed-70201892020-03-09 New EST-SSR Markers for Individual Genotyping of Opium Poppy Cultivars (Papaver somniferum L.) Vašek, Jakub Čílová, Daniela Melounová, Martina Svoboda, Pavel Vejl, Pavel Štikarová, Radka Vostrý, Luboš Kuchtová, Perla Ovesná, Jaroslava Plants (Basel) Article High-quality simple sequence repeat (SSR) markers are invaluable tools for revealing genetic variability which could be utilized for many purposes, such as breeding new varieties or the identifying current ones, among other applications. Based on the analysis of 3.7 million EST sequences and 15 genomic sequences from bacterial artificial chromosome (BAC) libraries, 200 trinucleotide genic (EST)-SSR and three genomic (gSSR) markers were tested, where 17 of them fulfilled all criteria for quality markers. Moreover, the reproducibility of these new markers was verified by two genetics laboratories, with a mean error rate per allele and per locus equal to 0.17%. These markers were tested on 38 accessions of Papaver somniferum and nine accessions of another five species of the Papaver and Argemone genera. In total, 118 alleles were detected for all accessions (median = 7; three to ten alleles per locus) and 88 alleles (median = 5; three to nine alleles per locus) within P. somniferum alone. Multivariate methods and identity analysis revealed high resolution capabilities of the new markers, where all but three pair accessions (41 out of 47) had a unique profile and opium poppy was distinguished from other species. MDPI 2019-12-19 /pmc/articles/PMC7020189/ /pubmed/31861643 http://dx.doi.org/10.3390/plants9010010 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vašek, Jakub
Čílová, Daniela
Melounová, Martina
Svoboda, Pavel
Vejl, Pavel
Štikarová, Radka
Vostrý, Luboš
Kuchtová, Perla
Ovesná, Jaroslava
New EST-SSR Markers for Individual Genotyping of Opium Poppy Cultivars (Papaver somniferum L.)
title New EST-SSR Markers for Individual Genotyping of Opium Poppy Cultivars (Papaver somniferum L.)
title_full New EST-SSR Markers for Individual Genotyping of Opium Poppy Cultivars (Papaver somniferum L.)
title_fullStr New EST-SSR Markers for Individual Genotyping of Opium Poppy Cultivars (Papaver somniferum L.)
title_full_unstemmed New EST-SSR Markers for Individual Genotyping of Opium Poppy Cultivars (Papaver somniferum L.)
title_short New EST-SSR Markers for Individual Genotyping of Opium Poppy Cultivars (Papaver somniferum L.)
title_sort new est-ssr markers for individual genotyping of opium poppy cultivars (papaver somniferum l.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020189/
https://www.ncbi.nlm.nih.gov/pubmed/31861643
http://dx.doi.org/10.3390/plants9010010
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