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Extraction, purification, characterization, anticoagulant activity, and anticoagulant mechanism of polysaccharides from the heads of Hypomesus olidus

The aim of this work was to extract, isolate, and purify polysaccharides from the heads of Hypomesus olidus and to evaluate their anticoagulant activities and anticoagulant mechanisms. Response surface methodology was used to optimize the extraction conditions for ultrasonic‐assisted extraction of p...

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Autores principales: Zhang, Xuan, Ma, Xin‐Tong, Xu, Yu, Jiang, Guo‐Chuan, Zhang, Jia‐Lin, Liu, Xue‐Jun, Yan, Xiao‐Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020297/
https://www.ncbi.nlm.nih.gov/pubmed/32148794
http://dx.doi.org/10.1002/fsn3.1360
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author Zhang, Xuan
Ma, Xin‐Tong
Xu, Yu
Jiang, Guo‐Chuan
Zhang, Jia‐Lin
Liu, Xue‐Jun
Yan, Xiao‐Hui
author_facet Zhang, Xuan
Ma, Xin‐Tong
Xu, Yu
Jiang, Guo‐Chuan
Zhang, Jia‐Lin
Liu, Xue‐Jun
Yan, Xiao‐Hui
author_sort Zhang, Xuan
collection PubMed
description The aim of this work was to extract, isolate, and purify polysaccharides from the heads of Hypomesus olidus and to evaluate their anticoagulant activities and anticoagulant mechanisms. Response surface methodology was used to optimize the extraction conditions for ultrasonic‐assisted extraction of polysaccharides from the heads of Hypomesus olidus. The optimal extraction conditions consisted of ultrasonic power of 275 W, ultrasonic time of 50 min, and solid–liquid ratio of 5 ml/g, giving the yield of crude polysaccharides (GYT) of 7.73 ± 0.042%. Three polysaccharide fractions, GYT‐1, GYT‐2, and GYT‐3 were purified from GYT by using DEAE‐cellulose‐52 column and Sephadex G‐100 column for anticoagulant activities. The results showed that two doses (2 and 4 mg/ml) of GYT‐1 and GYT‐3 could significantly prolong (p < .01) in partial thromboplastin time (APTT) (2.19 and 2.37 times, 2.22 and 2.44 times, respectively) and thrombin time (TT) (2.39 and 2.46 times, 2.44 and 2.80 times, respectively) compared with normal control. In particular, GYT‐3 had stronger anticoagulant activity than GYT‐1, and it was composed of arabinose, fructose, glucose, and lactose with molar ratios of 0.595:1: 2.026:0.273. However, GYT‐2 had no anticoagulant activity (p > .05). In addition, anticoagulation mechanism of polysaccharides from the heads of Hypomesus olidus (GYT‐3) was evaluated. The results showed that the anticoagulant activity of GYT‐3 was based on their binding with antithrombin AT‐III. And the inhibitory effects of GYT‐3 on factor IIa and Xa were related to the concentration of AT‐III in plasma. This study may provide a new and promising anticoagulant drug.
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spelling pubmed-70202972020-03-06 Extraction, purification, characterization, anticoagulant activity, and anticoagulant mechanism of polysaccharides from the heads of Hypomesus olidus Zhang, Xuan Ma, Xin‐Tong Xu, Yu Jiang, Guo‐Chuan Zhang, Jia‐Lin Liu, Xue‐Jun Yan, Xiao‐Hui Food Sci Nutr Original Research The aim of this work was to extract, isolate, and purify polysaccharides from the heads of Hypomesus olidus and to evaluate their anticoagulant activities and anticoagulant mechanisms. Response surface methodology was used to optimize the extraction conditions for ultrasonic‐assisted extraction of polysaccharides from the heads of Hypomesus olidus. The optimal extraction conditions consisted of ultrasonic power of 275 W, ultrasonic time of 50 min, and solid–liquid ratio of 5 ml/g, giving the yield of crude polysaccharides (GYT) of 7.73 ± 0.042%. Three polysaccharide fractions, GYT‐1, GYT‐2, and GYT‐3 were purified from GYT by using DEAE‐cellulose‐52 column and Sephadex G‐100 column for anticoagulant activities. The results showed that two doses (2 and 4 mg/ml) of GYT‐1 and GYT‐3 could significantly prolong (p < .01) in partial thromboplastin time (APTT) (2.19 and 2.37 times, 2.22 and 2.44 times, respectively) and thrombin time (TT) (2.39 and 2.46 times, 2.44 and 2.80 times, respectively) compared with normal control. In particular, GYT‐3 had stronger anticoagulant activity than GYT‐1, and it was composed of arabinose, fructose, glucose, and lactose with molar ratios of 0.595:1: 2.026:0.273. However, GYT‐2 had no anticoagulant activity (p > .05). In addition, anticoagulation mechanism of polysaccharides from the heads of Hypomesus olidus (GYT‐3) was evaluated. The results showed that the anticoagulant activity of GYT‐3 was based on their binding with antithrombin AT‐III. And the inhibitory effects of GYT‐3 on factor IIa and Xa were related to the concentration of AT‐III in plasma. This study may provide a new and promising anticoagulant drug. John Wiley and Sons Inc. 2019-12-28 /pmc/articles/PMC7020297/ /pubmed/32148794 http://dx.doi.org/10.1002/fsn3.1360 Text en © 2019 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Zhang, Xuan
Ma, Xin‐Tong
Xu, Yu
Jiang, Guo‐Chuan
Zhang, Jia‐Lin
Liu, Xue‐Jun
Yan, Xiao‐Hui
Extraction, purification, characterization, anticoagulant activity, and anticoagulant mechanism of polysaccharides from the heads of Hypomesus olidus
title Extraction, purification, characterization, anticoagulant activity, and anticoagulant mechanism of polysaccharides from the heads of Hypomesus olidus
title_full Extraction, purification, characterization, anticoagulant activity, and anticoagulant mechanism of polysaccharides from the heads of Hypomesus olidus
title_fullStr Extraction, purification, characterization, anticoagulant activity, and anticoagulant mechanism of polysaccharides from the heads of Hypomesus olidus
title_full_unstemmed Extraction, purification, characterization, anticoagulant activity, and anticoagulant mechanism of polysaccharides from the heads of Hypomesus olidus
title_short Extraction, purification, characterization, anticoagulant activity, and anticoagulant mechanism of polysaccharides from the heads of Hypomesus olidus
title_sort extraction, purification, characterization, anticoagulant activity, and anticoagulant mechanism of polysaccharides from the heads of hypomesus olidus
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020297/
https://www.ncbi.nlm.nih.gov/pubmed/32148794
http://dx.doi.org/10.1002/fsn3.1360
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