Cargando…
Honokiol, an active compound of Magnolia officinalis, is involved in restoring normal baroreflex sensitivity in hypercholesterolemic rabbits
This study investigated the effects of methanol extract Magnolia officinalis (MEMO) on baroreceptor reflex sensitivity (BRS) in the hypercholesterolemic rabbits and the involved molecular mechanisms. Male New Zealand white rabbits were randomly divided into Control (normal diet), Cholesterol (0.5% w...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020318/ https://www.ncbi.nlm.nih.gov/pubmed/32148818 http://dx.doi.org/10.1002/fsn3.1395 |
_version_ | 1783497719668539392 |
---|---|
author | Chou, Pei‐Yu Chang, Weng‐Cheng Liu, Fon‐Chang Lan, Shou‐Jen Sheu, Ming‐Jyh Chen, Jwo‐Sheng |
author_facet | Chou, Pei‐Yu Chang, Weng‐Cheng Liu, Fon‐Chang Lan, Shou‐Jen Sheu, Ming‐Jyh Chen, Jwo‐Sheng |
author_sort | Chou, Pei‐Yu |
collection | PubMed |
description | This study investigated the effects of methanol extract Magnolia officinalis (MEMO) on baroreceptor reflex sensitivity (BRS) in the hypercholesterolemic rabbits and the involved molecular mechanisms. Male New Zealand white rabbits were randomly divided into Control (normal diet), Cholesterol (0.5% w/w cholesterol diet), and Magnolia groups (0.5% w/w cholesterol diet plus 1% w/w MEMO). The animals were treated with the designated diet for 4 or 8 weeks. BRS in the control of heart rate was assessed by linear regression method. After 8 weeks of treatments, plasma total cholesterol (TC) was significantly elevated in the Cholesterol/Magnolia groups. The arterial blood pressure (aBP) was increased in the Cholesterol and Magnolia groups. The depression of BRS observed in the Cholesterol group was significantly ameliorated in the Magnolia group. After L‐NAME (Nω‐nitro‐Larginine methyl ester, 20 mg/kg, iv), the BRS of the Cholesterol group was significantly improved. Results from our in vitro study further indicated that honokiol, the principle component of MEMO, would protect human umbilical vein endothelial cells (HUVECs) from H(2)O(2)‐induced damages and inhibit H(2)O(2)‐induced vascular smooth muscles cells (VSMCs) proliferation, which was evident by the decreased expression of pFAK, and p‐Erk1/2. The results of the present study suggested that the improvement of BRS by MEMO in the hypercholesterolemic rabbits might be mediated by the antioxidant property of MEMO as indicated by the results from the L‐NAME and in vitro honokiol studies. |
format | Online Article Text |
id | pubmed-7020318 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70203182020-03-06 Honokiol, an active compound of Magnolia officinalis, is involved in restoring normal baroreflex sensitivity in hypercholesterolemic rabbits Chou, Pei‐Yu Chang, Weng‐Cheng Liu, Fon‐Chang Lan, Shou‐Jen Sheu, Ming‐Jyh Chen, Jwo‐Sheng Food Sci Nutr Original Research This study investigated the effects of methanol extract Magnolia officinalis (MEMO) on baroreceptor reflex sensitivity (BRS) in the hypercholesterolemic rabbits and the involved molecular mechanisms. Male New Zealand white rabbits were randomly divided into Control (normal diet), Cholesterol (0.5% w/w cholesterol diet), and Magnolia groups (0.5% w/w cholesterol diet plus 1% w/w MEMO). The animals were treated with the designated diet for 4 or 8 weeks. BRS in the control of heart rate was assessed by linear regression method. After 8 weeks of treatments, plasma total cholesterol (TC) was significantly elevated in the Cholesterol/Magnolia groups. The arterial blood pressure (aBP) was increased in the Cholesterol and Magnolia groups. The depression of BRS observed in the Cholesterol group was significantly ameliorated in the Magnolia group. After L‐NAME (Nω‐nitro‐Larginine methyl ester, 20 mg/kg, iv), the BRS of the Cholesterol group was significantly improved. Results from our in vitro study further indicated that honokiol, the principle component of MEMO, would protect human umbilical vein endothelial cells (HUVECs) from H(2)O(2)‐induced damages and inhibit H(2)O(2)‐induced vascular smooth muscles cells (VSMCs) proliferation, which was evident by the decreased expression of pFAK, and p‐Erk1/2. The results of the present study suggested that the improvement of BRS by MEMO in the hypercholesterolemic rabbits might be mediated by the antioxidant property of MEMO as indicated by the results from the L‐NAME and in vitro honokiol studies. John Wiley and Sons Inc. 2020-01-14 /pmc/articles/PMC7020318/ /pubmed/32148818 http://dx.doi.org/10.1002/fsn3.1395 Text en © 2020 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Chou, Pei‐Yu Chang, Weng‐Cheng Liu, Fon‐Chang Lan, Shou‐Jen Sheu, Ming‐Jyh Chen, Jwo‐Sheng Honokiol, an active compound of Magnolia officinalis, is involved in restoring normal baroreflex sensitivity in hypercholesterolemic rabbits |
title | Honokiol, an active compound of Magnolia officinalis, is involved in restoring normal baroreflex sensitivity in hypercholesterolemic rabbits |
title_full | Honokiol, an active compound of Magnolia officinalis, is involved in restoring normal baroreflex sensitivity in hypercholesterolemic rabbits |
title_fullStr | Honokiol, an active compound of Magnolia officinalis, is involved in restoring normal baroreflex sensitivity in hypercholesterolemic rabbits |
title_full_unstemmed | Honokiol, an active compound of Magnolia officinalis, is involved in restoring normal baroreflex sensitivity in hypercholesterolemic rabbits |
title_short | Honokiol, an active compound of Magnolia officinalis, is involved in restoring normal baroreflex sensitivity in hypercholesterolemic rabbits |
title_sort | honokiol, an active compound of magnolia officinalis, is involved in restoring normal baroreflex sensitivity in hypercholesterolemic rabbits |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020318/ https://www.ncbi.nlm.nih.gov/pubmed/32148818 http://dx.doi.org/10.1002/fsn3.1395 |
work_keys_str_mv | AT choupeiyu honokiolanactivecompoundofmagnoliaofficinalisisinvolvedinrestoringnormalbaroreflexsensitivityinhypercholesterolemicrabbits AT changwengcheng honokiolanactivecompoundofmagnoliaofficinalisisinvolvedinrestoringnormalbaroreflexsensitivityinhypercholesterolemicrabbits AT liufonchang honokiolanactivecompoundofmagnoliaofficinalisisinvolvedinrestoringnormalbaroreflexsensitivityinhypercholesterolemicrabbits AT lanshoujen honokiolanactivecompoundofmagnoliaofficinalisisinvolvedinrestoringnormalbaroreflexsensitivityinhypercholesterolemicrabbits AT sheumingjyh honokiolanactivecompoundofmagnoliaofficinalisisinvolvedinrestoringnormalbaroreflexsensitivityinhypercholesterolemicrabbits AT chenjwosheng honokiolanactivecompoundofmagnoliaofficinalisisinvolvedinrestoringnormalbaroreflexsensitivityinhypercholesterolemicrabbits |